Iron as a stabilizer of vitamin B12 activity in liver extracts and the nature of so-called alkali-stable factor

Studies on zinc toxicity in the larvae of the rice moth, Corcyra cephalonica St

“…The alkali-stable fraction of liver extract was prepared by adjusting the extract to pH 11-12 and autoclaving it for 30 min. at 1200 (Shenoy & Ramasarma, 1955).…”

Relative Amounts of Vitamin B "12" in Detritus from Oceanic and Estuarine Environments Near Sapelo Island, Georgia

“…Macek and Feller ( 1955) found only a slight loss on heating at 120° C for 30 min. Shenoy and Ramasarma ( 1955) reported that the stability of the vitamin to prolonged heating depends on the inorganic milieu. They showed that iron gave excellent protection to the B12 activity of liver extracts when heated at pH 10.0 at 120° C for 30 min.…”

Effects of Treatment with Food Additives on Nutrients

“…Natural materials such as liver contain sufficient iron to stabilize vitamin B12 as shown by Shenoy and Ramasarma (1955). The stability of Bll in foods during processing will depend on the content of iron and chelates.…”