Iron complex-based fluorescent probes for intracellular hydrogen peroxide detection

Hitomi, Yutaka; Takeyasu, Toshiyuki; Kodera, Masahito

doi:10.1039/c3cc44471f

Cited by 36 publications

(23 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared with the fluorescence in the cells before stimulation, fluorescence imaging showed a fluorescence decrease in the blue channel and a remarkable emission increase in the green channel (Figure ). The result is consistent with that previously reported in the literature . These data indicate that CNP is capable of imaging endogenous H 2 O 2 in living A431 cells.…”

Section: Resultssupporting

confidence: 93%

Dual Mechanism of an Intramolecular Charge Transfer (ICT)–FRET‐Based Fluorescent Probe for the Selective Detection of Hydrogen Peroxide

Shang

Qiao

et al. 2017

Chemistry An Asian Journal

View full text Add to dashboard Cite

A dual-mechanism intramolecular charge transfer (ICT)-FRET fluorescent probe for the selective detection of H O in living cells has been designed and synthesized. This probe used a coumarin-naphthalimide hybrid as the FRET platform and a boronate moiety as the recognition group. Upon the addition of H O , the probe exhibited a redshifted (73 nm) fluorescence emission, and the ratio of fluorescence intensities at λ=558 and 485 nm (F /F ) shifted notably (up to 100-fold). Moreover, there was a good linearity (R =0.9911) between the ratio and concentration of H O in the range of 0 to 60 μm, with a limit of detection of 0.28 μm (signal to noise ratio (S/N)=3). This probe could also detect enzymatically generated H O . Importantly, it could be used to visualize endogenous H O produced by stimulation from epidermal growth factor.

show abstract

Section: Resultssupporting

confidence: 93%

Dual Mechanism of an Intramolecular Charge Transfer (ICT)–FRET‐Based Fluorescent Probe for the Selective Detection of Hydrogen Peroxide

Shang

Qiao

et al. 2017

Chemistry An Asian Journal

View full text Add to dashboard Cite

show abstract

“…As one of the important fluorescent reporters, resorufin, a highly fluorescent molecule at pH 8 ( ϕ F =0.75), has excitation/emission maxima of ∼570/585 nm, and there is much less interference from autofluorescence in most biological samples. N‐Protected 3,7‐dihydroxyphenoxazine and O‐alkyl resorufin derivatives have been described as chemical probes for enzymes in the literature. As a precursor of a fluorescent probe, the former has an advantage of lower fluorescence background, which is less than that of the latter because of an interrupted conjugated system.…”

Section: Methodsmentioning

confidence: 99%

A 3,7‐Dihydroxyphenoxazine‐based Fluorescent Probe for Selective Detection of Intracellular Hydrogen Peroxide

Han

Liang

Ren

et al. 2016

Chemistry An Asian Journal

View full text Add to dashboard Cite

A novel N-borylbenzyloxycarbonyl-3,7-dihydroxyphenoxazine fluorescent probe (NBCD) for detecting H2O2 in living cells is described. The probe could achieve high selectivity for detecting H2O2 over other biological reactive oxygen species (ROS). In addition, upon addition of H2O2, NBCD exhibited color change from colorless to pink, which makes it a "naked-eye" probe for H2O2 detection. NBCD could not only be used to detect enzymatically generated H2O2 but also to detect H2O2 in living systems by using fluorescence spectroscopy, with a detection limit of 2 μm. Importantly, NBCD enabled the visualization of epidermal growth factor (EGF)-stimulated H2O2 generation inside the cells.

show abstract

“…A number of pioneering researchers have realized the imaging of ROS in living cells such as macrophages, cervical cancer cells, gastric cancer cells, and liver cancer cells, as well as in mouse and angelfish models, based on the design and synthesis of fluorescent small-molecular probes. [41,[111][112][113][114][115][116] In addition, molecular probes enabling the imaging of both H 2 O 2 and thiols in cells and tissues, [117,118] as well as AIE-based fluorescence nanoparticles capable of in vivo H 2 O 2 imaging, have been developed. [119] Li and co-workers [120] have developed a rhodamine-derived probe for H 2 O 2 imaging in HeLa cells.…”

Section: Fluorescent Imaging Agents For Hydrogen Peroxidementioning

confidence: 99%

Sensors, Imaging Agents, and Theranostics to Help Understand and Treat Reactive Oxygen Species Related Diseases

et al. 2019

View full text Add to dashboard Cite

Reactive oxygen species (ROS) consist of a diverse range of oxidative small molecular ions and free radicals that are produced throughout the body during certain biological processes. Due to their high reactivity, these molecules result in the damage of tissues and cells. Therefore, ROS have been implicated in an array of diseases including cancer, inflammation, and neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Owing to the simplicity, sensitivity, and selectivity of fluorescence‐based strategies, many small‐molecular sensors or imaging agents have been developed to sense and visualize ROS both in vitro and in vivo. Likewise, activatable drug delivery and prodrug systems that can be triggered by ROS for disease theranostics (diagnostic and therapeutic combined) have been developed. Herein, recently developed fluorescence‐based sensing and imaging agents for the detection of ROS both intracellularly and in vivo are summarized. In addition, drug delivery, which require activation by ROS to achieve disease theranostics is also discussed.

show abstract

Iron complex-based fluorescent probes for intracellular hydrogen peroxide detection

Abstract: A metal-based fluorescent probe for H2O2, named MBFh2, releases a highly fluorescent resorufin in the seconds time scale even in the presence of 5 μM H2O2. The use of MBFh2 enabled the visualization of intracellular H2O2 that was generated after stimulation of the epidermal growth factor.

Cited by 36 publications

References 27 publications

Dual Mechanism of an Intramolecular Charge Transfer (ICT)–FRET‐Based Fluorescent Probe for the Selective Detection of Hydrogen Peroxide

Dual Mechanism of an Intramolecular Charge Transfer (ICT)–FRET‐Based Fluorescent Probe for the Selective Detection of Hydrogen Peroxide

A 3,7‐Dihydroxyphenoxazine‐based Fluorescent Probe for Selective Detection of Intracellular Hydrogen Peroxide

Sensors, Imaging Agents, and Theranostics to Help Understand and Treat Reactive Oxygen Species Related Diseases

Contact Info

Product

Resources

About