Iron determination in osteoblast‐like cell culture medium by adsorptive cathodic stripping voltammetry with a mercury microelectrode

Morais, Simone; Carvalho, Graça Simões de; Sousa, João Paulo Barreto de

doi:10.1002/elan.1140091012

Cited by 15 publications

(14 citation statements)

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“…In the presented studies, adsorptive stripping voltammetry with catechol as a complexing agent was used for iron determination, whereas reference measurements were carried out by ET-AAS. The method employing catechol was used to determine iron in a wide range of samples: seawater [6], wine [7], osteoblast-like cell culture medium [8], and simulated PWR coolant [9]. Among other complexing agents used for iron determinations which utilize AdSV methods, the following ones should be listed: 1-nitroso-2-naphthol [10, 11], solochrome violet RS [12].…”

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Application of Adsorptive Stripping Voltammetry for Iron Determination with Catechol in Quartz and Silica Glass Samples

Gawryś¹,

Golimowski

1999

Electroanalysis

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A procedure for iron determination in quartz and silica glass materials based on adsorptive stripping voltammetry (AdSV) in the presence of catechol is described. Hanging mercury drop electrode as a working electrode was used. The optimized conditions include pH 7.1, accumulation potential À0.1 V, accumulation time 60 s, scan rate 10 mV s À1, pulse amplitude 25 mV. In case of 60 s accumulation time the obtained detection limit was 2. 2 mg L À1 Fe. The possibility of simultaneous determination of iron and copper in the same solution is shown. ET-AAS technique was applied as a reference method to AdSV measurements.Keywords: Adsorptive stripping voltammetry, Iron, Catechol, Quartz, Silica glass Silica glass is a high purity product containing 99.9 % of silica and even the content of impurities at ppm level can change its optical properties remarkably. Therefore the quality of the raw materials used in its production is of great importance. Within a group of natural materials quartz crystals ®nd the most frequent use. Typical iron content in natural quartz ranges from fractions of ppm to several ppm.Synthetic silica glass practically does not contain any impurities, and for this reason it is transparent for UV radiation up from 180 nm. In case of the silica glass obtained from natural materials this threshold is shifted towards longer waves. This shift as well as the decrease in UV radiation transmittance are generally attributed to iron ions [1]. The absorption of UV radiation by iron ions is probably caused by the absorption bands speci®c for this element (d±d electron transitions). The higher the iron content, the more evident these effects become [2].There are two kinds of analytical methods used for iron determination in quartz and silica glass samples, the ones without a decomposition step and the others requiring sample decomposition. In the ®rst group of methods XRF technique is widely used. Yet, the high detection limit of this method restricts its applications. In the second group, iron content can be measured by means of AAS or spectrophotometric methods. The variation of AAS method which involves sample feeding in form of à slurry' has gained much attention among analytical chemists in the recent years [3,4]. Application of voltammetric stripping techniques for iron determination in quartz or silica glass samples has not been found in the literature. However, AdSV method was applied to determination of titanium in quartz samples [5]. In the presented studies, adsorptive stripping voltammetry with catechol as a complexing agent was used for iron determination, whereas reference measurements were carried out by ET-AAS. The method employing catechol was used to determine iron in a wide range of samples: seawater [6], wine [7], osteoblast-like cell culture medium [8], and simulated PWR coolant [9]. Among other complexing agents used for iron determinations which utilize AdSV methods, the following ones should be listed: 1-nitroso-2-naphthol [10, 11], solochrome violet RS [12].The aim of this article w...

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confidence: 99%

Application of Adsorptive Stripping Voltammetry for Iron Determination with Catechol in Quartz and Silica Glass Samples

Gawryś¹,

Golimowski

1999

Electroanalysis

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“…The application of a square-wave waveform to ultramicro-electrodes presents very special features, namely: (1) the ohmic drops are greatly diminished, so sample treatment is minimized and in situ assays become possible; (2) the capacitive charging currents are highly reduced, allowing a better discrimination of the faradaic current; (3) the reduced values of resistance and capacitance of ultramicroelectrodes lower the cell-time constant, and therefore allow the working electrode to apply the potential on very short time scales; (4) the small size of the ultramicroelectrodes enables analysis of very small sample volumes. These points have been documented in connection with trace analyses of: several metals in in vitro cell cultures [32][33][34][35][36][37]; the anti-depressant venlafaxine in urine [38]; pesticides in soils [39,40].…”

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confidence: 99%

Determination of ametryn in soils via microwave-assisted solvent extraction coupled to anodic stripping voltammetry with a gold ultramicroelectrode

et al. 2005

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An extraction-anodic adsorptive stripping vol-tammetric procedure using microwave-assisted solvent extraction and a gold ultramicroelectrode was developed for determining the pesticide ametryn in soil samples. The method is based on the use of acetonitrile as extraction solvent and on controlled adsorptive accumulation of the herbicide at the potential of 0.50 V (vs. Ag/AgCl) in the presence of Britton-Robinson buffer (pH 3.3). Soil sample extracts were analysed directly after drying and redis-solution with the supporting electrolyte but without other pre-treatment. The limit of detection obtained for a 10 s collection time was 0.021 lg g -1. Recovery experi-ments for the global procedure, at the 0.500 lg g -1 level, gave satisfactory mean and standard deviation results which were comparable to those obtained by HPLC with UV detection.

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“…Adsorptive voltammetry, which has been proven suitable for the quanti-fication of a large variety of biologically significant organic molecules, [7][8] when coupled with the inherent properties of microelectrodes [9][10] has shown to be a reliable and promising technique. [11][12][13] …”

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Anodic Adsorptive Stripping Voltammetric Determination of Atrazine in Spiked Soil Samples with a Gold Microelectrode

et al. 2004

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An adsorptive stripping voltammetric procedure for the determina-tion of the antidepressant venlafaxine in urine using a mercury film microelectrode was developed. The method is based on controlled adsorptive accumulation of the drug at the potential of -1.00 V (vs. Ag/AgCl) in the presence of 1.25 x 10 -2 mol L -1 borate buffer (pH 8.7). Urine samples were analyzed directly after performing a ten-fold dilution with the supporting electrolyte but without other pretreatment. The limit of detection obtained for a 30 s collection time was 0.693 x 10 -6 mol L -1 . Recovery experiments gave good results at the 10 -6 mol L -1 level (bias less 5% were obtained).

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Iron determination in osteoblast‐like cell culture medium by adsorptive cathodic stripping voltammetry with a mercury microelectrode

Cited by 15 publications

References 33 publications

Application of Adsorptive Stripping Voltammetry for Iron Determination with Catechol in Quartz and Silica Glass Samples

Application of Adsorptive Stripping Voltammetry for Iron Determination with Catechol in Quartz and Silica Glass Samples

Determination of ametryn in soils via microwave-assisted solvent extraction coupled to anodic stripping voltammetry with a gold ultramicroelectrode

Anodic Adsorptive Stripping Voltammetric Determination of Atrazine in Spiked Soil Samples with a Gold Microelectrode

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