Iron(iii) citrate speciation in aqueous solution

Silva, André M. N.; Kong, Xiaole; Parkin, Mark C.; Cammack, Richard; Hider, Robert C.

doi:10.1039/b910970f

Cited by 216 publications

(222 citation statements)

References 30 publications

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“…S6B). Because there is no consensus in the literature on the affinity constants for the different Fe(III) citrate complexes, we did not attempt to calculate the speciation of Fe(III) citrate precisely (43).…”

Section: Methodsmentioning

confidence: 99%

Central role for ferritin in the day/night regulation of iron homeostasis in marine phytoplankton

Botebol

Lesuisse

Šuták

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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In large regions of the open ocean, iron is a limiting resource for phytoplankton. The reduction of iron quota and the recycling of internal iron pools are among the diverse strategies that phytoplankton have evolved to allow them to grow under chronically low ambient iron levels. Phytoplankton species also have evolved strategies to cope with sporadic iron supply such as long-term storage of iron in ferritin. In the picophytoplanktonic species Ostreococcus we report evidence from observations both in the field and in laboratory cultures that ferritin and the main ironbinding proteins involved in photosynthesis and nitrate assimilation pathways show opposite diurnal expression patterns, with ferritin being maximally expressed during the night. Biochemical and physiological experiments using a ferritin knock-out line subsequently revealed that this protein plays a central role in the diel regulation of iron uptake and recycling and that this regulation of iron homeostasis is essential for cell survival under iron limitation.

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Section: Methodsmentioning

confidence: 99%

Central role for ferritin in the day/night regulation of iron homeostasis in marine phytoplankton

Botebol

Lesuisse

Šuták

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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“…This discrepancy might result from the fact that thermodynamic equilibrium cannot be reached within this short period of time (30 min). Also, the chemistry of ferric citrate speciation in aqueous solution is complicated and not fully understood yet, and the estimated affinity constants for the monoiron dicitrate complex ranges from (log) 19.1 to 38.7 in the literature (Silva et al, 2009). As we worked with concentrated cell suspensions, predictions on iron speciation should also account for the charges and potential ligands of metals introduced in the system by the cells themselves.…”

Section: Iron Uptake Assays: Speciation Of Ironmentioning

confidence: 99%

Nonreductive Iron Uptake Mechanism in the Marine Alveolate Chromera velia

et al. 2010

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Chromera velia is a newly cultured photosynthetic marine alveolate. This microalga has a high iron requirement for respiration and photosynthesis, although its natural environment contains less than 1 nM of this metal. We found that this organism uses a novel mechanism of iron uptake, differing from the classic reductive and siderophore-mediated iron uptake systems characterized in the model yeast Saccharomyces cerevisiae and present in most yeasts and terrestrial plants. C. velia has no transplasma membrane electron transfer system, and thus cannot reduce extracellular ferric chelates. It is also unable to use hydroxamate siderophores as iron sources. Iron uptake from ferric citrate by C. velia is not inhibited by a ferrous chelator, but the rate of uptake is strongly decreased by increasing the ferric ligand (citrate) concentration. The cell wall contains a large number of iron binding sites, allowing the cells to concentrate iron in the vicinity of the transport sites. We describe a model of iron uptake in which aqueous ferric ions are first concentrated in the cell wall before being taken up by the cells without prior reduction. We discuss our results in relation to the strategies used by the phytoplankton to take up iron in the oceans.

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“…monomeric and oligomeric Fe(III) complexes. 8,16 The fraction of plasma NTBI that is redox active and can be chelated is designated labile plasma iron (LPI). 12,13 Iron complexes assumed to represent NTBI have been shown experimentally to be taken up by susceptible cell types, including hepatocytes, cardiomyocytes and pancreatic islet cells, with consequent oxidant injury.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4][5] Plasma NTBI apparently comprises several subspecies, which may be classified by their chemical composition, chemical reactivity or susceptibility to chelation. [6][7][8][9][10][11][12][13][14][15] The chemical composition of NTBI is heterogeneous and it is thought that there are several circulating isoforms, that is Fe(III) bound to albumin and citrate and potentially to acetate, malate and phosphate. [6][7][8]14 Of these, citrate has the highest affinity for Fe(III), and under physiological conditions two isoforms dominate, i.e.…”

Section: Introductionmentioning

confidence: 99%

Second international round robin for the quantification of serum non-transferrin-bound iron and labile plasma iron in patients with iron-overload disorders

Swart¹,

Hendriks²,

Vorm³

et al. 2015

Haematologica

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N on-transferrin-bound iron and its labile (redox active) plasma iron component are thought to be potentially toxic forms of iron originally identified in the serum of patients with iron overload. We compared ten worldwide leading assays (6 for non-transferrinbound iron and 4 for labile plasma iron) as part of an international interlaboratory study. Serum samples from 60 patients with four different iron-overload disorders in various treatment phases were coded and sent in duplicate for analysis to five different laboratories worldwide. Some laboratories provided multiple assays. Overall, highest assay levels were observed for patients with untreated hereditary hemochromatosis and β-thalassemia intermedia, patients with transfusion-dependent myelodysplastic syndromes and patients with transfusion-dependent and chelated β-thalassemia major. Absolute levels differed considerably between assays and were lower for labile plasma iron than for non-transferrin-bound iron. Four assays also reported negative values. Assays were reproducible with high between-sample and low withinsample variation. Assays correlated and correlations were highest within the group of non-transferrin-bound iron assays and within that of labile plasma iron assays. Increased transferrin saturation, but not ferritin, was a good indicator of the presence of forms of circulating nontransferrin-bound iron. The possibility of using non-transferrin-bound iron and labile plasma iron measures as clinical indicators of overt iron overload and/or of treatment efficacy would largely depend on the rigorous validation and standardization of assays. Second international round robin for the quantification of serum non-transferrin-bound iron and labile plasma iron in patients with iron-overload disorders

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Iron(iii) citrate speciation in aqueous solution

Cited by 216 publications

References 30 publications

Central role for ferritin in the day/night regulation of iron homeostasis in marine phytoplankton

Central role for ferritin in the day/night regulation of iron homeostasis in marine phytoplankton

Nonreductive Iron Uptake Mechanism in the Marine Alveolate Chromera velia

Second international round robin for the quantification of serum non-transferrin-bound iron and labile plasma iron in patients with iron-overload disorders

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