Iron(III)‐Selective Chelation‐Enhanced Fluorescence Sensing for In Vivo Imaging Applications

Guo, Lin; Liu, Xin Yi; Wang, Huan; Chen, Qiu Ling; Wang, Guang Ke; Luo, Kongjia; Mi, Qi Li; Zhou, Ying; Zhang, Jun Feng

doi:10.1002/asia.201500415

Cited by 17 publications

(5 citation statements)

References 35 publications

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“…In contrast, iron supplementation significantly upregulated protein levels of ferritin in worms grown on either E. coli OP50 or S. Typhimurium (Fig 4D and 4H). Next, we used RDN3, a ferric (Fe 3+ )-selective fluorescent sensor [34], to label the ferric form. We found that the ferric foci stained by this dye were merged into ferritin, as detected by immunofluorescence (S6 Fig) . Thus, the ferric foci mainly represent iron in ferritin.…”

Section: Plos Geneticsmentioning

confidence: 99%

“…For detecting fluorescent signals in worms, ftn-1p::pes-10::gfp-his and ftn-2p::pes-10::gfp-his strains were mounted in M9 onto microscope slides. Ferric (Fe 3+ )-selective fluorescent sensor RDN3 were used to stain label the ferric form stored in worms according to the method described previously [34]. The worms were analyzed using a Zeiss Axioskop 2 Plus fluorescence microscope (Carl Zeiss, Jena, Germany).…”

Section: Microscopymentioning

confidence: 99%

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TOR functions as a molecular switch connecting an iron cue with host innate defense against bacterial infection

Dai

Qiu

et al. 2021

PLoS Genet

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As both host and pathogen require iron for survival, iron is an important regulator of host-pathogen interactions. However, the molecular mechanism by which how the availability of iron modulates host innate immunity against bacterial infections remains largely unknown. Using the metazoan Caenorhabditis elegans as a model, we demonstrate that infection with a pathogenic bacterium Salmonella enterica serovar Typhimurium induces autophagy by inactivating the target of rapamycin (TOR). Although the transcripts of ftn-1 and ftn-2 encoding two H-ferritin subunits are upregulated upon S. Typhimurium infection, the ferritin protein is kept at a low level due to its degradation mediated by autophagy. Autophagy, but not ferritin, is required for defense against S. Typhimurium infection under normal circumstances. Increased abundance of iron suppresses autophagy by activating TOR, leading to an increase in the ferritin protein level. Iron sequestration, but not autophagy, becomes pivotal to protect the host from S. Typhimurium infection in the presence of exogenous iron. Our results show that TOR acts as a regulator linking iron availability with host defense against bacterial infection.

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Section: Plos Geneticsmentioning

confidence: 99%

Section: Microscopymentioning

confidence: 99%

TOR functions as a molecular switch connecting an iron cue with host innate defense against bacterial infection

Dai

Qiu

et al. 2021

PLoS Genet

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“…Hence, detection of Zn II from drinking water is essential. Among various detection procedures, chelation enhanced fluorescence (CHEF) is highly effective, as it provides fluorescence enhancement with or without any spectral shifts after chelation with specific metal ions. − Hence the presence of metal ion could be easily detected by naked eye.…”

Section: Introductionmentioning

confidence: 99%

Coordination Polymer Gels with Modular Nanomorphologies, Tunable Emissions, and Stimuli-Responsive Behavior Based on an Amphiphilic Tripodal Gelator

Sutar

Maji

2017

Inorg. Chem.

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The recent upsurge in research on coordination polymer gels (CPGs) stems from their synthetic modularity, nanoscale processability, and versatile functionalities. Here we report self-assembly of an amphiphilic, tripodal low-molecular weight gelator (L) that consists of 4,4',4-[1,3,5-phenyl-tri(methoxy)]-tris-benzene core and 2,2':6',2″-terpyridyl termini, with different metal ions toward the formation of CPGs that show controllable nanomorphologies, tunable emission, and stimuli-responsive behaviors. L can also act as a selective chemosensor for Zn with very low limit of detection (0.18 ppm) in aqueous medium. Coordination-driven self-assembly of L with Zn in HO/MeOH solvent mixture results in a coordination polymer hydrogel (ZnL) that exhibits sheet like morphology and charge-transfer emission. On the other hand, coordination of L with Tb and Eu in CHCl/tetrahydrofuran solvent mixture results in green- and red-emissive CPGs, respectively, with nanotubular morphology. Moreover, precise stoichiometric control of L/Eu/Tb ratio leads to the formation of bimetallic CPGs that show emissions over a broad spectral range, including white-light-emission. We also explore the multistimuli responsive properties of the white-light-emitting CPG by exploiting the dynamics of Ln-tpy coordination.

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“…N‐terminal‐elongated (Nte) residues of Inx2 and Inx3 can be made by using Bac‐to‐Bac system containing NT‐6His‐TEV to produce reBac‐Nte‐Inxs. At 24‐h post‐infection (PI), hemichannel closes, endogenous phosphate ion accumulates, and Fe 3+ is enriched in reBac‐Nte‐Inx3 (Guo et al., , ; Zhang et al., ).…”

Section: Introductionmentioning

confidence: 99%

N‐TERMINALLY ELONGATED SpliInx2 AND SpliInx3 REDUCE BACULOVIRUS‐TRIGGERED APOPTOSIS VIA HEMICHANNEL CLOSURE

Chen

Xiao

et al. 2016

Arch Insect Biochem Physiol

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The hemichannel and gap junction channel are major portals for the release of factors responsible for the effects of apoptotic cells on the spread of apoptosis to neighboring cells and apoptotic corpse clearance, typically by phagocytes. The N-terminal cytoplasmic domain in the connexins, gap junction proteins in vertebrate, has been implicated in regulating channel closure. However, little is known about how the hemichannel close responds to apoptotic signaling transduction leading to the reduction of neighboring cellular apoptosis in an invertebrate. An insect Bac-to-Bac expression system, pFastBac(TM) HT A, allows us to construct an N-terminally elongated SpliInx2 (Nte-Inx2) and SpliInx3 (Nte-Inx3). Here, we demonstrated that recombinant baculovirus Bac-Nte-Inx2 (reBac-Net-Inx2) and Bac-Nte-Inx3 (reBac-Nte-Inx3) closed the endogenous hemichannel on the Sf9 cell surface. Importantly, primary baculovirus infections significantly caused early apoptosis, and this apoptosis was reduced by hemichannel-closed Sf9 cells at 24-h post-infection (PI). Although N-terminal-elongated residue led to the increase in the phosphorylated sites in both Nte-Inx2 and Nte-Inx3 and an additional transmembrane domain in Nte-Inx3, both the proteins localized on the cell surface, suggesting Nte-Inxs proteins could mediate hemichannel closure. Further supporting evidence showed that hemichannel closure was dependent on N-Inxs expressed by baculovirus polyhedrin promoter, which began to express at 18-24 h PI. These results identify an unconventional function of N-terminal-elongated innexins that could act as a plug to manipulate hemichannel closure and provide a mechanism connecting the effect of hemichannel closure directly to apoptotic signaling transduction from intracellular to extracellular compartment.

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Iron(III)‐Selective Chelation‐Enhanced Fluorescence Sensing for In Vivo Imaging Applications

Cited by 17 publications

References 35 publications

TOR functions as a molecular switch connecting an iron cue with host innate defense against bacterial infection

TOR functions as a molecular switch connecting an iron cue with host innate defense against bacterial infection

Coordination Polymer Gels with Modular Nanomorphologies, Tunable Emissions, and Stimuli-Responsive Behavior Based on an Amphiphilic Tripodal Gelator

N‐TERMINALLY ELONGATED SpliInx2 AND SpliInx3 REDUCE BACULOVIRUS‐TRIGGERED APOPTOSIS VIA HEMICHANNEL CLOSURE

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