Iron oxide nanoparticles and induced autophagy in human monocytes

Wu, Qi; Jin, Rong; Feng, Ting; Liu, Li; Yang, Li; Tao, Yu Hong; Anderson, James M.; Ai, Hua; Li, Hong

doi:10.2147/ijn.s135189

Cited by 51 publications

(37 citation statements)

References 53 publications

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“…In this study, AgNP synthesized by chemical reduction of silver nitrate (AgNO 3 ) using sodium borohydride (NaBH 4 ) was encapsulated in a DPPC/cholesterol liposome to both stabilize and improve the uptake of the AgNP in vitro for enhanced cytotoxicity. Two simple encapsulation methods were trialed AgNP, followed by nanoparticle characterization and evaluation of cytotoxicity on a THP1 cell line, a monocytic cell line which acts as first line of Defense against nanoparticle during exposure [10] .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of silver nanoparticle encapsulation in DPPC-based liposome by different methods for enhanced cytotoxicity

Yusef¹,

Casey²

2019

International Journal of Polymeric Materials and Polymeric Biom

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Here we carried out a comparative study on two cost and time effective methods of encapsulating silver nanoparticles (AgNP) in dipalmitoyl-phosphatidyl choline (DPPC)/cholesterol-based liposome to enhance its cytotoxicity and reduce cytotoxic concentrations and evaluated the effect of this on a blood cell line (THP1 monocytes) often involved in uptake of nanoparticles during human exposure. DLS and Zeta potential analyses over a 6-months period showed the extruded Lipo-AgNP (ExLipo-AgNP) exhibited more stable characteristics when compared with the probe-sonicated Lipo-AgNP (PB-Lipo-AgNP). SEM microscopy indicated agglomeration of the PB-Lipo-AgNP which was not observed in Ex-Lipo-AgNP. Ex-Lipo-AgNP also exhibited higher temperaturedependent stability with 35.3% reduction in size from 20 C to 37 C while PB-Lipo-AgNP was less stable exhibiting 55% size reduction over same temperature range. Load release study over 24 h showed a controlled load release from Ex-Lipo-AgNP while the PB-Lipo-AgNP exhibited burst release at pH 4 and 6.5. Interestingly, it was found that Ex-Lipo-AgNP induced significantly higher toxicity on THP1 cell line after 24 h exposure compared with control unexposed cells; uncoated AgNP and PB-Lipo-AgNP exposed cells at the same concentration. Thus, for the first time, we report that liposomal encapsulation of AgNP by extrusion produces a stable nanocapsule with enhanced cytotoxicity, thus preventing overreliance on high AgNP concentration to achieve desired toxicity.

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Section: Introductionmentioning

confidence: 99%

Evaluation of silver nanoparticle encapsulation in DPPC-based liposome by different methods for enhanced cytotoxicity

Yusef¹,

Casey²

2019

International Journal of Polymeric Materials and Polymeric Biom

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“…54 Autophagy was induced in human monocytes by iron oxide superparamagnetic NPs. 55 In contrast, silver NPs have been shown to impede autophagy in monocytes. 56 Of note, majority of studies use standard and popular cancer cell lines to test Abbreviation: FDA, US Food and Drug Administration.…”

Section: Cytotoxicitymentioning

confidence: 99%

Design Considerations and Assays for Hemocompatibility of FDA-Approved Nanoparticles

Saha

Zhen

Erogbogbo

et al. 2019

Semin Thromb Hemost

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Nanoparticles have numerous biomedical applications including, but not limited to, targeted drug delivery, diagnostic imaging, sensors, and implants for a wide range of diseases including cancer, diabetes, heart disease, and tuberculosis. Although the mode of delivery of the nanoparticles depends on the application and the disease, the nanoparticles are often in immediate contact with the systemic circulation either because of intravenous administration or their ability to enter the bloodstream with relative ease or their longer survival time in circulation. Once in circulation, the nanoparticles may elicit unintended hemostatic and inflammatory responses, and hence the design of nanoparticles for therapeutic applications should take broad hemocompatibility concerns into consideration. In this review, we present the principles underlying the structural and functional design of various classes of nanoparticles that are currently approved by the US Food and Drug Administration, categorize these particles based on their interactions with cardiovascular tissues and ensuing adverse events, and also describe various in vitro assays that may be used evaluate their hemocompatibility.

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“…Under normal conditions, cells transfected with a GFP-LC3-encoding construct exhibit a diffuse fluorescence spread throughout the cytoplasm. However, when the number of autophagosomes increases, they can be identified as distinct puncta [175,181,188]. Alternatively, colocalization assays of NPls with autophagy markers such as LC3 are frequently used to evaluate the role of autophagy in NPl-mediated gene delivery [17].…”

Section: Autophagymentioning

confidence: 99%

Methodologies to investigate intracellular barriers for nucleic acid delivery in non-viral gene therapy

et al. 2018

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A plethora of biological barriers, intended to defend tissues and cells against external influences, stand in the way of efficient nucleic acid delivery by non-viral nanoplexes. Even when nanoplexes successfully evade extracellular barriers and reach their target cell, many intracellular barriers remain to be conquered. These include overcoming the plasma membrane, evading endosomal compartmentalization, and in some cases crossing the nuclear envelope. At the same time, exocytosis, autophagy and cytoplasmic degradation of the cargo should be avoided. Currently, there is a growing appreciation that the interaction of nanoplexes with these barriers should be understood in detail in order to rationally design a second generation of non-viral nanoplexes, capable of overcoming these many hurdles. Studying intracellular biobarriers is, however, quite challenging and specialized methods are constantly being developed. In this review, we present an overview of established as well as emerging techniques and assays that are currently available to the experimentalist to study nanoplexbarrier interaction, with a focus on quantitative methods.

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Iron oxide nanoparticles and induced autophagy in human monocytes

Cited by 51 publications

References 53 publications

Evaluation of silver nanoparticle encapsulation in DPPC-based liposome by different methods for enhanced cytotoxicity

Evaluation of silver nanoparticle encapsulation in DPPC-based liposome by different methods for enhanced cytotoxicity

Design Considerations and Assays for Hemocompatibility of FDA-Approved Nanoparticles

Methodologies to investigate intracellular barriers for nucleic acid delivery in non-viral gene therapy

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