Iron Oxide Nanoparticles as an Alternative to Antibiotics Additive on Extended Boar Semen

Tsakmakidis, Ιoannis; Samaras, Theodoros; Anastasiadou, Sofia; Basioura, Athina; Ntemka, Aikaterini; Michos, Ilias; Simeonidis, K.; Karagiannis, I.; Tsousis, Georgios; Angelakeris, M.; Boscos, C.

doi:10.3390/nano10081568

Cited by 19 publications

(17 citation statements)

References 49 publications

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“…Their activity appeared to be preventive for Staphylococcus spp., Enterococcus spp., and Enterobacteriaceae, and in some samples the microbial reduction was intense; however, the bacterial counts ranged among samples, indicating a non-repetitive result. These findings are not aligned with the reduction of the bacterial load previously described by our research team [ 9 ], regarding the antibacterial action of iron NPs on boar semen. Further investigation is necessary to clarify the factors that suppressed the antimicrobial properties of the NPs in the present study.…”

Section: Discussioncontrasting

confidence: 94%

“…Taking into consideration that in sheep practice AI with fresh cooled semen should be performed within 6–8 h after semen extension [ 25 ], the use of NPs could be justified. In our previous study, no toxic effect was observed for Fe 3 O 4 NPs on boar spermatozoa even after 48 h of storage [ 9 ]. Among the adverse effects of NPs, differences regarding cells’ susceptibility are acknowledged, thus in vitro tests for Nano safety show particular attention to cell type selection [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…Sperm morphology was evaluated by applying the SpermBlue staining protocol (SpermBlue ® 08029, Microptic S.L., Barcelona, Spain) following the manufacturer’s instructions [ 9 ]. Spermatozoa were assessed microscopically (×400) and classified as normal or with morphological abnormalities [head (including integrity of acrosome membrane), tail, cytoplasmic droplets].…”

Section: Methodsmentioning

confidence: 99%

“…This is a before used valid scientific approach regarding the interaction and the consequences of increasing doses of NPs on major sperm kinetic variables of ram sperm [ 18 ]. Considering that collection of ram semen has a higher contamination risk compared to boar semen collection, as well as the previously used doses of Fe 3 O 4 (0.192 mg per mL) and Ag/Fe NPs (0.128 mg per mL) on boar semen [ 9 , 24 ], multiplied (×2, ×4, ×8, ×16, ×32) doses of NPs were tested. Eight ejaculates ( n = 8; 1 ejaculate/ram) were used in pretrial.…”

Section: Methodsmentioning

confidence: 99%

“…In this context, the use of colloidal solutions to naturally remove bacteria by centrifugation [ 5 ], the supplementation of peptides of natural or synthetic origin in semen extenders [ 6 , 7 ], and the use of natural products with antibacterial activity, such as honey [ 8 ], have been reported. Promising results have been published by our scientific team, regarding the use of iron oxide NPs as alternatives to antibiotic additives in boar semen [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Toxic and Microbiological Effects of Iron Oxide and Silver Nanoparticles as Additives on Extended Ram Semen

Tsakmakidis

Samaras

Anastasiadou

et al. 2021

Animals

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The aim of the study was to investigate the effect of iron oxide (Fe) and silver (Ag) nanoparticles (NPs) on ram semen. A skim milk extender without antibiotics was used as a diluent of 21 ejaculates (8 rams; 2–3 ejaculates/ram). The groups of control (C; semen without NPs), Fe NPs (3.072 mg Fe3O4/mL semen), and Ag NPs (2.048 mg Ag-Fe/mL semen) were incubated (15 °C; 30 min), and then a magnetic field was used for NPs’ removal. Standard microbiological procedures were performed for all groups. Post-treated samples were stored (15 °C) for 24 h, and sperm variables (kinetics by computer assisted sperm analysis (CASA); viability; morphology; HOST; DNA integrity) were evaluated at 6 and 24 h. Semen data were analyzed by a mixed model for repeated measures and microbiological data with Student’s t-test for paired samples. At 6 h of storage, VCL and rapid movement-spermatozoa, and at 24 h, total/progressive motility and amplitude of lateral head displacement (ALH) were significantly decreased in group Ag compared to control. In group Fe, progressive/rapid movement-spermatozoa were significantly lower compared to control after 24 h of storage. Only in group Ag was a significant reduction of total bacterial count revealed. In conclusion, the examined Fe NPs demonstrated slight antibacterial effect, while the examined Ag NPs provided higher antibacterial properties accompanied by cytotoxicity.

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Section: Discussioncontrasting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Toxic and Microbiological Effects of Iron Oxide and Silver Nanoparticles as Additives on Extended Ram Semen

Tsakmakidis

Samaras

Anastasiadou

et al. 2021

Animals

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Preparation and characterization of glucose‐conjugated super‐paramagnetic iron oxide nanoparticles (G‐SPIONs) for removal of Edwardsiella tarda and Aeromonas hydrophila from water

Kumar

Gupta

Varghese

et al. 2022

Microscopy Res & Technique

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The present research was conducted to prepare efficient G‐SPIONs by co‐precipitation to remove Edwardsiella tarda and Aeromonas hydrophila from the aqueous solution. The synthesized G‐SPIONs were characterized by UV–Vis spectrophotometer, DLS, FEG‐TEM, FT‐IR, XRD, and VSM analysis. The results showed that the synthesized G‐SPIONs had super‐paramagnetic properties (58.31 emu/g) and spherical shape (16 ± 3 nm). The antibacterial activity was assessed in sterilized distilled water at different G‐SPIONs concentrations viz. 0, 1.5, 3, 6, 12, 24, 48, 120, and 240 mg/L against E. tarda and A. hydrophila with various bacterial loads viz. 1 × 103, 1 × 104, 1 × 105, 1 × 106, and 1 × 107 CFU/ml at different time intervals 15, 30, 45, and 60 min. At a lower bacterial load of E. tarda and A. hydrophila 1 × 103–1 × 104 CFU/ml, 100% bacterial load was removed by 15 min exposure with NPs concentration 6–48 mg/L and 1.5–6 mg/L, respectively. Cent percent bacterial removal was observed in both the bacterial species even at higher bacterial load (1 × 105–1 × 107 CFU/ml) by increasing exposure time (15–60 min) and nanoparticle concentration as well (24–240 mg/L). At an initial bacterial load of E. tarda and A. hydrophila (1 × 103–1 × 107 CFU/ml), the EC50 ranged between 0.01–6.51 mg/L and 0.02–3.84 mg/L, respectively, after 15–60 min exposure. Thus, it is concluded that the antibacterial effect of G‐SPIONs depends on concentration and exposure time. Hence, G‐SPIONs can be used as an antibacterial/biocidal agent to treat Edwardsiellosis and Aeromonosis disease in aquaculture. Highlights The glucose‐conjugated super‐paramagnetic iron oxide nanoparticles (G‐SPIONs) synthesized by rapid and high yield co‐precipitation method shows bacterial removal efficiency against Edwardsiella tarda and Aeromonas hydrophila The bacterial removal efficiency of G‐SPIONs depends on concentration and exposure time to both the bacterial species The 100% bacterial removal efficiency can be achieved even at the highest bacterial load (1 × 107 CFU/ml) against E. tarda and A. hydrophila after 45 and 30 min exposure Due to the high bacterial removal efficiency of G‐SPIONs against E. tarda and A. hydrophila, it can be used for the treatment of Edwardsiellosis and Aeromonosis in fish

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The toxicity of superparamagnetic iron oxide nanoparticles induced on the testicular cells: In vitro study

Dantas,

Ferraz,

Coimbra

et al. 2024

NanoImpact

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Iron Oxide Nanoparticles as an Alternative to Antibiotics Additive on Extended Boar Semen

Cited by 19 publications

References 49 publications

Toxic and Microbiological Effects of Iron Oxide and Silver Nanoparticles as Additives on Extended Ram Semen

Toxic and Microbiological Effects of Iron Oxide and Silver Nanoparticles as Additives on Extended Ram Semen

Preparation and characterization of glucose‐conjugated super‐paramagnetic iron oxide nanoparticles (G‐SPIONs) for removal of Edwardsiella tarda and Aeromonas hydrophila from water

The toxicity of superparamagnetic iron oxide nanoparticles induced on the testicular cells: In vitro study

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