Iron Regulates Microglial Cell-Mediated Secretory and Effector Functions

Saleppico, Silvia; Mazzolla, Rosanna; Boelaert, Johan R.; Puliti, Manuela; Barluzzi, Roberta; Bistoni, Francesco; Blasi, Elisabetta

doi:10.1006/cimm.1996.0159

Cited by 40 publications

(27 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the presence of iron, a decrease in LPS-induced NO production from HAPI cells was observed. A similar effect of iron on NO production was observed in mouse microglia (BV2) (Saleppico et al, 1996). Our observations were also consistent with a current report, showing that exposure of activated murine macrophages to iron decreased iNOS transcript, as well as its enzyme activity (Weiss et al, 1994).…”

Section: Discussionsupporting

confidence: 93%

Characterization of a novel brain‐derived microglial cell line isolated from neonatal rat brain

Cheepsunthorn

Radov

Menzies

et al. 2001

Glia

148

114

View full text Add to dashboard Cite

We observed highly aggressively proliferating immortalized (HAPI) cells growing in cultures that had been enriched for microglia. The cells were initially obtained from mixed glial cultures prepared from 3-day-old rat brains. HAPI cells are typically round with few or no processes when cultured in 10% serum containing medium. As the percentage of serum in the medium is decreased, the HAPI cells have more processes. HAPI cells stain for the isolectin B4, OX-42, and GLUT5, which are markers for microglial cells, but the cells do not immunolabel with A2B5, a marker of cells in the oligodendroglial cell lineage, or with the astrocyte-specific marker, glial fibrillary aciidic protein (GFAP). In addition, HAPI cells are capable of phagocytosis. We conclude that HAPI cells are of microglia/macrophage lineage. Exposing HAPI cells to lipopolysaccharide (LPS) induces the mRNAs for tumor necrosis factor-alpha (TNF-alpha) and inducible nitric oxide synthase (iNOS). LPS exposure also induces secretion of TNF-alpha and production of nitric oxide (NO) in HAPI cells. Because activation of microglia is associated with an increase in iron accumulation and ferritin expression, we tested the hypothesis that iron status affects the production of TNF-alpha and NO. Our studies demonstrate that both iron chelation and iron loading diminished the LPS-induced effect of TNF-alpha and NO. The results of this study indicate that HAPI cells possess the characteristics of microglia/brain macrophages, providing an alternative cell culture model for the study of microglia. In addition, we demonstrate that the activation of microglial cells could be modified by iron.

show abstract

Section: Discussionsupporting

confidence: 93%

Characterization of a novel brain‐derived microglial cell line isolated from neonatal rat brain

Cheepsunthorn

Radov

Menzies

et al. 2001

Glia

148

114

View full text Add to dashboard Cite

show abstract

“…An excellent example is bacterial siderophores. Bacterial siderophores take iron from transferrins produced by a variety of host species (Dlaska and Weiss, 1999;Saleppico et al, 1996;Tanji et al, 2001;Weiss et al, 1994). Along with this line, previous reports demonstrated that intestinal opportunistic E. coli may cause diseases by releasing siderophores (Der Vartanian et al, 1992), and certain Vibrio sp.…”

Section: Introductionmentioning

confidence: 93%

“…mutants which have defects in iron transportation or acquisition show reduced pathogenicity in mice models (Henderson and Payne, 1994;Kurz et al, 2003;Wong et al, 1996). Therefore, it is not surprising that iron chelators alter immune status and regulate inflammatory processes (Dlaska and Weiss, 1999;Saleppico et al, 1996;Tanji et al, 2001;Weiss et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Involvement of protein kinase Cδ in iron chelator-induced IL-8 production in human intestinal epithelial cells

Choi

Lee

et al. 2007

Life Sciences

View full text Add to dashboard Cite

“…TNF-a a Secretion The production of TNF-a by the macrophages was quantified by a bioassay using L929 cells, as described by Saleppico et al 12) Briefly, L929 cells were seeded into a 96-well plate (4ϫ10 4 cells/well) and maintained at 37°C under a humidified atmosphere of 95% air and 5% CO 2 . After the 24-h incubation, the medium (90 ml) containing actinomycin D (1 mg/ml) was freshly replaced.…”

Section: Methodsmentioning

confidence: 99%

In Vitro and in Vivo Effects of Macrophage-Stimulatory Polysaccharide from Leaves of Perilla frutescens var. crispa.

Kwon

Kim

Jun

et al. 2002

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

The crude polysaccharide (PFB-1) was isolated from the leaves of Perilla frutescens var. crispa by the sequential procedures with hot-water extraction, methanol reflux, and ethanol precipitation. It was further purified by anion column chromatography in order to obtain the partially purified polysaccharide (PFB-1-0). In the presence of PFB-1-0, strong cellular lysosomal enzyme activity of murine peritoneal macrophages was observed in vitro. Compared to bacterial lipopolysaccharide (LPS), its activity was relatively high. The in vitro phagocytic activity was enhanced by PFB-1-0 as the similar pattern in both gram-negative bacteria, E. coli, and gram-positive bacteria, S. aureus with a time-dependent manner. We also investigated the production of several mediators by murine peritoneal macrophages upon stimulation with PFB-1 (in vivo) or PFB-1-0 (in vitro). The levels of nitric oxide (NO) and tumor necrosis factor (TNF)-a a were increased in the presence of PFB-1-0 in vitro. The PFB-1 stimulated the production of interleukin (IL)-6 and granulocyte-macrophage colony-stimulating factor (GM-CSF) in vivo. Results suggest that the polysaccharide from P. frutescens var. crispa represents an immunopotentiator and biological response modifiers in vitro and in vivo levels.

show abstract

Iron Regulates Microglial Cell-Mediated Secretory and Effector Functions

Cited by 40 publications

References 0 publications

Characterization of a novel brain‐derived microglial cell line isolated from neonatal rat brain

Characterization of a novel brain‐derived microglial cell line isolated from neonatal rat brain

Involvement of protein kinase Cδ in iron chelator-induced IL-8 production in human intestinal epithelial cells

In Vitro and in Vivo Effects of Macrophage-Stimulatory Polysaccharide from Leaves of Perilla frutescens var. crispa.

Contact Info

Product

Resources

About