1981
DOI: 10.1093/nar/9.11.2629
|View full text |Cite
|
Sign up to set email alerts
|

Iron-related modification of bacterial transfer RNA

Abstract: Transfer RNAs isolated from E. coli grown in media where ferric iron is not freely available show well characterized chromatographic changes due to the absence of the methylthio moiety of ms2i6A. The altered tRNA molecules include tRNA trp tRNA tyr, tRNA phe and two minor tRNA ser species. It has been suggested that methylthiolation of tRNA affects its function in regulation. We now show iron-related changes in tRNA trp from S. typhimurium, Ps. aeruginosa and K. pneumoniae. tRNA trp from S. typhimurium contain… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

1
11
0

Year Published

1983
1983
2020
2020

Publication Types

Select...
8
1

Relationship

0
9

Authors

Journals

citations
Cited by 22 publications
(12 citation statements)
references
References 18 publications
1
11
0
Order By: Relevance
“…In E. coli grown in iron-restricted conditions, tRNA species for phenylalanine, tyrosine, tryptophan, serine, cysteine and leucine lack the methylthio group normally present on an isopentenyl-adenosine residue located next to the anticodon. Similar tRNA changes have been observed in Salmonella typhimurium, Klebsiella pneumoniae, Pseudomonas aeruginosa [222] and Neisseria meningitidis [223] grown in iron-restricted conditions. Such under-modification of tRNA induces pleiotropic effects on cell physiology, affecting translational efficiency, codon context [224][225][226][227][228][229][230], increased aromatic amino acid and enterochelin synthesis, and aromatic amino acid transport [224,225,231].…”
Section: Iron-responsit;e Genetic Regulation In Microorganismssupporting
confidence: 78%
“…In E. coli grown in iron-restricted conditions, tRNA species for phenylalanine, tyrosine, tryptophan, serine, cysteine and leucine lack the methylthio group normally present on an isopentenyl-adenosine residue located next to the anticodon. Similar tRNA changes have been observed in Salmonella typhimurium, Klebsiella pneumoniae, Pseudomonas aeruginosa [222] and Neisseria meningitidis [223] grown in iron-restricted conditions. Such under-modification of tRNA induces pleiotropic effects on cell physiology, affecting translational efficiency, codon context [224][225][226][227][228][229][230], increased aromatic amino acid and enterochelin synthesis, and aromatic amino acid transport [224,225,231].…”
Section: Iron-responsit;e Genetic Regulation In Microorganismssupporting
confidence: 78%
“…On the contrary, the most frequently used codons for serine and leucine are read by tRNAs that are not dependent on the isopentenylating enzyme; while cysteine is infrequently used in most proteins and thus, should not influence much overall protein synthesis. Similar arguments may apply to the growth of E.coli in iron deficient media which results in a drastic reduction of methylthiolation of i6A (39). The methyl-2-thio group is indeed a major factor in the stabilization of anticodon-anticodon interactions of ms2i6A37 containing tRNAs (6).…”
Section: Cga'mentioning
confidence: 81%
“…During iron-restricted growth of E. coli or in miaA mutants, the complete modification of a specific adenosine in the anticodon loop of some species of tRNAs to form 2-methylthioisopentenyladenosine does not occur (7,22,34). Such undermodified tRNAs have been shown to be less efficient in translation of RNAs containing contiguous codons recognized by these molecules (7,34).…”
Section: Resultsmentioning
confidence: 99%