Three beakers for removing Fe (II) in reconstituted water (doped with FeSO 4 ) were built and tested. Given the set operating conditions ([O 2 ] > 4 mg·L −1 , P atm = 1.013 bar, T = 25˚C ± 1˚C and [Fe 2+ ] 0 = 0.5 to 2 mg·L −1 ), removal of iron was caused by biological and possibly physical and chemical oxidation because there is a quantity of free oxygen in the medium. The extent of each type of oxidation has not been evaluated as it specifically studies the biological degradation of iron in these beaker tests by setting the operating conditions (pH > 6.5, dissolved oxygen from 0 to 8 mg·L −1 , Redox Potential from 100 to 400 mV). The experimental studies focused particularly on the measurements of maximum wavelength, conversion efficiencies from Fe (II) to Fe (III), the effect of the Fe (II) concentration, the influence of pH, the action of the temperature of the prepared solutions and the effect of O 2 concentration under specified operating conditions. It noticed precipitated amounts of iron deposited at the bottom of the beakers. Thus, the low concentrations of Fe (II) detected in the influent after the biological oxidation operation could be attributed to microorganisms that consume iron as a substrate.