Ironing out the issues: Integrated approaches to understanding iron homeostasis in plants

Samira, Rozalynne; Stallmann, Anna; Massenburg, Lynnicia; Long, Terri A.

doi:10.1016/j.plantsci.2013.06.004

Cited by 14 publications

(6 citation statements)

References 87 publications

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“…The protein encoded by this tomato gene shows highest homology with Arabidopsis thaliana MYB48 (35 % of identity) that is not reported to be involved in responses to Fe-deficiency in that plant species. Until now, in Strategy I plants, the regulation of Fe-deficiency responses has been described to be controlled by bHLH transcription factors [ 1 , 40 ]. Data here presented suggest that responses to Fe supply after a period of shortage could be driven by other transcription factors such as this MYB.…”

Section: Resultsmentioning

confidence: 99%

Early transcriptomic response to Fe supply in Fe-deficient tomato plants is strongly influenced by the nature of the chelating agent

et al. 2016

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BackgroundIt is well known that in the rhizosphere soluble Fe sources available for plants are mainly represented by a mixture of complexes between the micronutrient and organic ligands such as carboxylates and phytosiderophores (PS) released by roots, as well as fractions of humified organic matter. The use by roots of these three natural Fe sources (Fe-citrate, Fe-PS and Fe complexed to water-extractable humic substances, Fe-WEHS) have been already studied at physiological level but the knowledge about the transcriptomic aspects is still lacking.ResultsThe 59Fe concentration recorded after 24 h in tissues of tomato Fe-deficient plants supplied with 59Fe complexed to WEHS reached values about 2 times higher than those measured in response to the supply with Fe-citrate and Fe-PS. However, after 1 h no differences among the three Fe-chelates were observed considering the 59Fe concentration and the root Fe(III) reduction activity. A large-scale transcriptional analysis of root tissue after 1 h of Fe supply showed that Fe-WEHS modulated only two transcripts leaving the transcriptome substantially identical to Fe-deficient plants. On the other hand, Fe-citrate and Fe-PS affected 728 and 408 transcripts, respectively, having 289 a similar transcriptional behaviour in response to both Fe sources.ConclusionsThe root transcriptional response to the Fe supply depends on the nature of chelating agents (WEHS, citrate and PS). The supply of Fe-citrate and Fe-PS showed not only a fast back regulation of molecular mechanisms modulated by Fe deficiency but also specific responses due to the uptake of the chelating molecule. Plants fed with Fe-WEHS did not show relevant changes in the root transcriptome with respect to the Fe-deficient plants, indicating that roots did not sense the restored cellular Fe accumulation.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-2331-5) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Early transcriptomic response to Fe supply in Fe-deficient tomato plants is strongly influenced by the nature of the chelating agent

et al. 2016

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“…This indicates that the major focus of new studies on foliar Fe fertilization should be put on internal Fe transport within the leaf, and new formulations should be aimed to extend the reach of the Fe fertilizers beyond the treated area. Whereas basic Fe transport mechanisms in the plant have been recently unraveled [see reviews by Abadía et al (2011) and Samira et al (2013)], including xylem Fe transport as Fe-carboxylate complexes (Rellán-Álvarez et al, 2010), Fe reduction by mesophyll cells (González-Vallejo et al, 2000; Larbi et al, 2001) and nicotianamine-dependent phloem Fe unloading (Schuler et al, 2012), very little is known on the Fe transport mechanisms occurring after foliar Fe fertilization. New knowledge on the Fe mobilization pathways in the Fe-fertilized leaves will be necessary to improve fertilization efficiency.…”

Section: Discussionmentioning

confidence: 99%

The effects of foliar fertilization with iron sulfate in chlorotic leaves are limited to the treated area. A study with peach trees (Prunus persica L. Batsch) grown in the field and sugar beet (Beta vulgaris L.) grown in hydroponics

et al. 2014

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Crop Fe deficiency is a worldwide problem. The aim of this study was to assess the effects of foliar Fe applications in two species grown in different environments: peach (Prunus persica L. Batsch) trees grown in the field and sugar beet (Beta vulgaris L. cv. “Orbis”) grown in hydroponics. The distal half of Fe-deficient, chlorotic leaves was treated with Fe sulfate by dipping and using a brush in peach trees and sugar beet plants, respectively. The re-greening of the distal (Fe-treated) and basal (untreated) leaf areas was monitored, and the nutrient and photosynthetic pigment composition of the two areas were also determined. Leaves were also studied using chlorophyll fluorescence imaging, low temperature-scanning electron microscopy microanalysis, scanning transmission ion microscopy-particle induced X-ray emission and Perls Fe staining. The distal, Fe-treated leaf parts of both species showed a significant increase in Fe concentrations (across the whole leaf volume) and marked re-greening, with significant increases in the concentrations of all photosynthetic pigments, as well as decreases in de-epoxidation of xanthophyll cycle carotenoids and increases in photochemical efficiency. In the basal, untreated leaf parts, Fe concentrations increased slightly, but little re-greening occurred. No changes in the concentrations of other nutrients were found. Foliar Fe fertilization was effective in re-greening treated leaf areas both in peach trees and sugar beet plants. Results indicate that the effects of foliar Fe-sulfate fertilization in Fe-deficient, chlorotic leaves were minor outside the leaf surface treated, indicating that Fe mobility within the leaf is a major constraint for full fertilizer effectiveness in crops where Fe-deficiency is established and leaf chlorosis occurs.

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“…Recent advances in high-resolution elemental analysis have been used to show changes in iron localization in indeterminate nodules (Rodríguez-Haas et al, 2013). Continued use of these and other elemental localization techniques (for review, see Samira et al, 2013; Zhao et al, 2014) such as energy-dispersive X-ray analysis or nanoSIMS to detect other elements should facilitate great strides in understanding metal distribution and translocation in SNF in the near future.…”

Section: Future Directionsmentioning

confidence: 99%

Fixating on metals: new insights into the role of metals in nodulation and symbiotic nitrogen fixation

et al. 2014

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Symbiotic nitrogen fixation is one of the most promising and immediate alternatives to the overuse of polluting nitrogen fertilizers for improving plant nutrition. At the core of this process are a number of metalloproteins that catalyze and provide energy for the conversion of atmospheric nitrogen to ammonia, eliminate free radicals produced by this process, and create the microaerobic conditions required by these reactions. In legumes, metal cofactors are provided to endosymbiotic rhizobia within root nodule cortical cells. However, low metal bioavailability is prevalent in most soils types, resulting in widespread plant metal deficiency and decreased nitrogen fixation capabilities. As a result, renewed efforts have been undertaken to identify the mechanisms governing metal delivery from soil to the rhizobia, and to determine how metals are used in the nodule and how they are recycled once the nodule is no longer functional. This effort is being aided by improved legume molecular biology tools (genome projects, mutant collections, and transformation methods), in addition to state-of-the-art metal visualization systems.

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Ironing out the issues: Integrated approaches to understanding iron homeostasis in plants

Cited by 14 publications

References 87 publications

Early transcriptomic response to Fe supply in Fe-deficient tomato plants is strongly influenced by the nature of the chelating agent

Early transcriptomic response to Fe supply in Fe-deficient tomato plants is strongly influenced by the nature of the chelating agent

The effects of foliar fertilization with iron sulfate in chlorotic leaves are limited to the treated area. A study with peach trees (Prunus persica L. Batsch) grown in the field and sugar beet (Beta vulgaris L.) grown in hydroponics

Fixating on metals: new insights into the role of metals in nodulation and symbiotic nitrogen fixation

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