Irradiated mesenchymal stem cells improve the ex vivo expansion of hematopoietic progenitors by partly mimicking the bone marrow endosteal environment

α2β1 Integrin Promotes Chemoresistance against Doxorubicin in Cancer Cells through Extracellular Signal-regulated Kinase (ERK)

“…Co-culture of Jurkat Cells with Bone Marrow Stromal CellsHuman bone marrow-derived mesenchymal stem cells (MSCs) were a generous gift from Dr. Nicholas Pineault (Hema-Qué-bec, Québec, Canada) and were described previously (20). The co-culture of Jurkat cells with MSCs and drug treatment was carried out as described previously (21,22).…”

Hematopoietic Niche – Exploring Biomimetic Cues to Improve the Functionality of Hematopoietic Stem/Progenitor Cells

“…Particularly, in the hematopoietic BM microenvironment, MSC are a major constituent, being in close association with HSPC and are thought to have an important HSPC supportive function through their capability to secrete hematopoietic cytokines . Although cytokine crosstalk between hematopoietic and mesenchymal cells has been pointed out by several authors, MSC‐HSPC contact also seems to be important to retain HSPC in their niche and favor maintenance of a more immature state . Of notice, although MSC were initially identified in the BM, these cells have been shown to be present in various tissues from either perinatal or adult sources .…”

Advances in umbilical cord blood stem cell expansion and clinical translation

“…Examples of such system are presented in Table 1 along with their principal properties and results. The major findings of these studies can be summarized as follow; i) coculture with stromal cells general increases the expansion and/or length of which HSPC proliferate [39,50,54], ii) coculture-expanded HSPCs retain phenotypes associated with primitive primary HSPCs [50,55,56], iii) exogenous cytokines synergize with stromal cells to promote HSPC expansion [49,50,[57][58][59][60][61][62], iv) both contact-dependent and contact-independent activities have been reported which suggest that the regulation of the HSPC compartment by stromal cells is multifaceted in nature [37,55,56,58,[63][64][65][66][67], v) coculture with stromal cells generally improves the engraftment activity of HSPCs vs. those expanded without stromal cells [37,39,[47][48][49][50][51]68], vi) coculture is more effective to promote the expansion of HSPCs from CB mononucleated cells (MNC) than stroma-free cultures [59,69,70] and, vii) M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 7 stromal cells secrete a wide array of cytokines and chemokines [49,55,65,[71]…”