Irradiation by high-intensity red light-emitting diode enhances human bone marrow mesenchymal stem cells osteogenic differentiation and mineralization through Wnt/β-catenin signaling pathway

Ruan, Yaru; Kato, Hirohito; Taguchi, Yoichiro; Yamauchi, Nobuhiro; Umeda, Makoto

doi:10.1007/s10103-020-03002-5

Cited by 24 publications

(17 citation statements)

References 48 publications

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“…Dot size represents the number of DEmRNAs in the corresponding GO term (bigger dots indicate larger DEmRNA numbers). may enhance the differentiation and mineralization of bone marrow MSCs (Ruan et al, 2021). These results suggest that metabolic alterations and MSCs differentiation in the bone marrow may be involved in the pathogenesis of T. canis infection at 12 hpi.…”

Section: Discussionmentioning

confidence: 67%

“…The col1a1 and col1a2 genes encode two α chains of the type i collagen, which is the major component of the bone matrix (Pollitt et al, 2006 ); three downregulated genes ( itpr1, nos3 , and pde1a ) and three upregulated genes ( grin2c, slc25a4 , and phka1 ) were enriched in calcium signaling pathway, which contributes to the osteogenic differentiation of MSCs (Hui et al, 2019 ). Furthermore, Wnt signaling pathway was significantly upregulated at 12 hpi, which may enhance the differentiation and mineralization of bone marrow MSCs (Ruan et al, 2021 ). These results suggest that metabolic alterations and MSCs differentiation in the bone marrow may be involved in the pathogenesis of T. canis infection at 12 hpi.…”

Section: Discussionmentioning

confidence: 99%

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Toxocara canis Infection Alters lncRNA and mRNA Expression Profiles of Dog Bone Marrow

Zheng

Zou

Liu

et al. 2021

Front. Cell Dev. Biol.

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Bone marrow is the main hematopoietic organ that produces red blood cells, granulocytes, monocyte/macrophages, megakaryocytes, lymphocytes, and myeloid dendritic cells. Many of these cells play roles in the pathogenesis of Toxocara canis infection, and understanding how infection alters the dynamics of transcription regulation in bone marrow is therefore critical for deciphering the global changes in the dog transcriptional signatures during T. canis infection. In this study, long non-coding RNA (lncRNA) and messenger RNA (mRNA) expression profiles in the bone marrow of Beagle dogs infected with T. canis were determined at 12 h post-infection (hpi), 24 hpi, 96 hpi, and 36 days post-infection (dpi). RNA-sequencing and bioinformatics analysis identified 1,098, 984, 1,120, and 1,305 differentially expressed lncRNAs (DElncRNAs), and 196, 253, 223, and 328 differentially expressed mRNAs (DEmRNAs) at 12 h, 24 h, 96 h, and 36 days after infection, respectively. We also identified 29, 36, 38, and 68 DEmRNAs potentially cis-regulated by 44, 44, 51, and 80 DElncRNAs at 12 hpi, 24 hpi, 96 hpi, and 36 dpi, respectively. To validate the sequencing findings, qRT-PCR was performed on 10 randomly selected transcripts. Many altered genes were involved in the differentiation of bone marrow cells. GO of DElncRNAs and GO and KEGG pathway analyses of DEmRNAs revealed alterations in several signaling pathways, including pathways involved in energy metabolism, amino acid biosynthesis and metabolism, Wnt signaling pathway, Huntington's disease, HIF-1 signaling pathway, cGMP–PKG signaling pathway, dilated cardiomyopathy, and adrenergic signaling in cardiomyocytes. These findings revealed that bone marrow of T. canis-infected dogs exhibits distinct lncRNA and mRNA expression patterns compared to healthy control dogs. Our data provide novel insights into T. canis interaction with the definitive host and shed light on the significance of the non-coding portion of the dog genome in the pathogenesis of toxocariasis.

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Toxocara canis Infection Alters lncRNA and mRNA Expression Profiles of Dog Bone Marrow

Zheng

Zou

Liu

et al. 2021

Front. Cell Dev. Biol.

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“…To study the effects of LED on astrocytes, a 660-nanometer LED was used ( Figure 1 A) with a power of 10 mW/cm 2 , duration of 10 min, and energy density of 6 J/cm 2 ( Table 1 ). Energy density was determined by referring to previous studies dealing with 660-nanometer PBM [ 22 , 23 , 24 ]. Cells were irradiated with the 660-nanometer LED in the dark.…”

Section: Methodsmentioning

confidence: 99%

Photobiomodulation with a 660-Nanometer Light-Emitting Diode Promotes Cell Proliferation in Astrocyte Culture

Yoon

Hong

Lee

et al. 2021

Cells

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Astrocytes act as neural stem cells (NSCs) that have the potential to self-renew and differentiate into other neuronal cells. The protein expression of these astrocytes depends on the stage of differentiation, showing sequential expression of multiple proteins such as octamer-binding transcription factor 4 (Oct4), nestin, glial fibrillary acidic protein (GFAP), and aldehyde dehydrogenase 1 family member L1 (aldh1L1). Photobiomodulation (PBM) affects cell apoptosis, proliferation, migration, and adhesion. We hypothesized that astrocyte proliferation and differentiation would be modulated by PBM. We used an optimized astrocyte culture method and a 660-nanometer light-emitting diode (LED) to enhance the biological actions of many kinds of cells. We determined that the 660-nanometer LED promoted the biological actions of cultured astrocytes by increasing the reactive oxygen species levels. The overall viability of the cultured cells, which included various cells other than astrocytes, did not change after LED exposure; however, astrocyte-specific proliferation was observed by the increased co-expression of GFAP and bromodeoxyuridine (BrdU)/Ki67. Furthermore, the 660-nanometer LED provides evidence of differentiation, as shown by the decreased Oct4 and GFAP co-expression and increased nestin and aldh1L1 expression. These results demonstrate that a 660-nanometer LED can modify astrocyte proliferation, which suggests the efficacy of the therapeutic application of LED in various pathological states of the central nervous system.

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“…The role of the Wnt/β‐catenin signaling pathway in the osteogenesis and adipogenesis of BMSCs has been confirmed by an increasing number of studies 54–57 . The Wnt/β‐catenin signaling pathway is thought to be a signal cascade reaction between LRP5, β‐catenin, and TCF.…”

Section: Discussionmentioning

confidence: 92%

“…The role of the Wnt/β-catenin signaling pathway in the osteogenesis and adipogenesis of BMSCs has been confirmed by an increasing number of studies. [54][55][56][57] The Wnt/β-catenin signaling pathway is thought to be a signal cascade reaction between LRP5, β-catenin, and TCF. Activation of LRP5 can increase the stable accumulation of β-catenin in the cytoplasm and thereby up-regulate the expression of TCF in the nucleus, which eventually increases the activation of the Wnt/β-catenin signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

LINC00473‐modified bone marrow mesenchymal stem cells incorporated thermosensitive PLGA hydrogel transplantation for steroid‐induced osteonecrosis of femoral head: A detailed mechanistic study and validity evaluation

Jiang

Wang

et al. 2021

Bioengineering & Transla Med

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The pathogenesis of steroid‐induced osteonecrosis of the femoral head (SONFH) involves a glucocorticoid‐induced imbalance of osteogenic and adipogenic differentiation, and apoptosis of bone marrow mesenchymal stem cells (BMSCs). An increasing number of genes, especially noncoding RNAs, have been implicated in the function of BMSCs. Our previous studies have confirmed the key role of LINC00473 and miR‐23a‐3p on the osteogenic, adipogenic differentiation, and apoptosis of BMSCs. However, the underlying mechanism of this process is still unclear. Based on bioinformatics analysis, here we investigated the effects of LINC00473 on the LRP5/Wnt/β‐catenin signaling pathway in the osteogenesis and adipogenesis of BMSCs, as well as the PEBP1/Akt/Bad/Bcl‐2 signaling pathway in dexamethasone‐ (Dex‐) induced apoptosis of BMSCs. Our data showed that LINC00473 could promote osteogenesis and suppress the adipogenesis of BMSCs through the activation of the miR‐23a‐3p/LRP5/Wnt/β‐catenin signaling pathway axis, while rescuing BMSCs from Dex‐induced apoptosis by activating the miR‐23a‐3p/PEBP1/Akt/Bad/Bcl‐2 signaling pathway axis. Notably, we observed that LINC00473 interacted with miR‐23a‐3p in an Argonaute 2 (AGO2)‐dependent manner based on dual‐luciferase reporter assay, AGO2‐related RNA immunoprecipitation, and RNA antisense purification assay. Furthermore, injectable thermosensitive polylactic‐co‐glycolic acid (PLGA) hydrogel loaded with rat‐derived BMSCs (rBMSCs) modified by LINC00473 were used for the treatment of SONFH in a rat model. Our results demonstrated that PLGA hydrogels provided a suitable environment for harboring rBMSCs. Besides, transplantation of PLGA hydrogels loaded with rBMSCs modified by LINC00473 could significantly promote the bone repair and reconstruction of the necrotic area at the femoral head in our SONFH rat model. Surprisingly, compared with the transplantation of BMSCs alone, the transplanted rBMSCs encapsulated within the PLGA hydrogel could migrate from the medullary cavity to the femoral head. In summary, LINC00473 promoted osteogenesis, inhibited adipogenesis, and antagonized Dex‐induced apoptosis of BMSCs. Therefore, LINC00473 could provide a new strategy for the treatment of SONFH.

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Irradiation by high-intensity red light-emitting diode enhances human bone marrow mesenchymal stem cells osteogenic differentiation and mineralization through Wnt/β-catenin signaling pathway

Cited by 24 publications

References 48 publications

Toxocara canis Infection Alters lncRNA and mRNA Expression Profiles of Dog Bone Marrow

Toxocara canis Infection Alters lncRNA and mRNA Expression Profiles of Dog Bone Marrow

Photobiomodulation with a 660-Nanometer Light-Emitting Diode Promotes Cell Proliferation in Astrocyte Culture

LINC00473‐modified bone marrow mesenchymal stem cells incorporated thermosensitive PLGA hydrogel transplantation for steroid‐induced osteonecrosis of femoral head: A detailed mechanistic study and validity evaluation

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