Irradiation Effects on Wound Contraction Using a Connective Tissue Model

Gamma-Irradiation Markedly Inhibits the Hydrated Collagen Gel Contraction by Arterial Smooth Muscle Cells

“…26 Another study that used nonirradiated collagen gels seeded with oral submucosal fibroblasts and irradiated them with single doses in the range of 2 to 10 Gy produced a similar dose-dependent inhibition of gel contraction. 6 These results support our findings that radiation effects on the survival and proliferation of SMC is likely responsible for the observed inhibition of collagen gel contraction. Our study was not designed to evaluate potential effects of irradiation on cell motion or on matrix protein synthesis/degradation, the latter of which may also play an important role in the inhibition of gel contraction.…”

“…The contraction of a collagen gel is a good model of wound healing because it reflects the combined effects of SMC proliferation, collagen matrix lysis and synthesis, and cell motion. In accordance with its inhibitory effect on wound healing in vivo, 4,5 irradiation is known to be a potent inhibitor of collagen gel contraction by human oral submucosal fibroblasts as demonstrated by Yanase et al 6 This study showed a significant dose-dependent reduction in gel contraction with doses in a range of 2 to 10 Gy. Although the vascular SMCs play a determining role in vessel wall responses after PTCA, the effects of irradiation on collagen gel contraction by SMCs are unknown; thus, the aim of this study was to evaluate the effects of different doses of gamma-irradiation on the contraction of a collagen gel by arterial SMCs, as well as to study cellular mechanisms responsible for such an effect.…”

“…To the best of our knowledge, the only study that has addressed the issue of the effects of irradiation on contraction of collagen gels is the study of Yanase et al 6 These authors evaluated the contraction of collagen gels seeded with human oral submucosal fibroblasts after irradiation with doses of 2 to 10 Gy. A significant dose-dependent inhibition of collagen gel contraction was seen from the second week after irradiation and during the subsequent 14 days; however, the difference in gel contraction between the irradiated groups decreased after 38 days of observation.…”

The effects of single doses of radiation on the wound healing behaviour of human Tenon's capsule fibroblasts

“…This finding is further supported by Yanase et al, who reported that irradiation of oral fibroblasts with x rays (200 to 1000 cGy) immediately after casting in collagen gels had no effect on either the rate or degree of contraction of the gels. 17 The fibroblasts in healing wounds are also important in synthesising new ECM molecules including fibronectin, which acts as a primary scaffold for cell attachment and migration, and collagen I and III which increase wound strength. 18 19 Our group has previously shown that hTf treated with growth arresting doses of 5-FU or MMC show a long term reduction in the production of fibronectin, collagen I and collagen III.…”

Reconstructive algorithms in the pediatric population

“…Studies have shown that radiation therapy also disrupts wound healing by impairing fibroblast proliferation and function . As a result, reconstructive procedures that rely on the defect site to significantly participate in the healing process tend to be fraught with higher complications and lower success rates.…”