Irradiation with 365 nm and 405 nm wavelength shows differences in DNA damage of swine pancreatic islets

Klak, Marta; Gomółka, Magdalena; Dobrzański, Tomasz; Tymicki, Grzegorz; Cywoniuk, Piotr; Kowalska, Patrycja; Kosowska, Katarzyna; Bryniarski, Tomasz; Berman, Andrzej; Dobrzyń, Agnieszka; Idaszek, Joanna; Święszkowski, Wojciech; Wszoła, Michał

doi:10.1371/journal.pone.0235052

Cited by 31 publications

(29 citation statements)

References 29 publications

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“…Compared to ultra‐violet light, 405 nm shows significantly less damage to cells and it is used as a lightning source in biological field. [ 78,79 ] Irradiated by light, the TC12 MMs of the TC/P microswimmer catalytically react with hydrogen peroxide to generate a large number of bubbles (the principle is shown in Equations (1)–(3)), and the bubble reaction force pushes the microswimmer to propel it in reverse, as shown in Figure A–C. As the region of TC/P microswimmers with more pores generates a large number of bubbles (marked as the tail) and the region with fewer pores generates a small number of bubbles (marked as the head), the microswimmers can be propelled by the large number of bubbles and swim in the direction of the head when the whole body of the microswimmer is illuminated by violet light.…”

Section: Resultsmentioning

confidence: 99%

3D‐Printed Light‐Driven Microswimmer with Built‐In Micromotors

Chen

Yang

et al. 2021

Adv Materials Technologies

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Untethered biocompatible microswimmers driven by micromotors in fluids can enable innovative technologies in fields such as biology and chemical and biomedical engineering. However, efficiently driving and controlling the movement of microswimmers by light remains a challenge. Herein, a method for fabricating a light‐driven microswimmer with built‐in micromotors and 3D‐printed multiscale features that exhibits high photocatalytic performance is proposed. TiO2‐CaCO3 composite microparticles (TC) are fabricated as highly efficient micromotors that provide high photocatalytic efficiency in bubble generation, while 3D‐printed hydrogels are fabricated as TiO2‐CaCO3/PEGDA (TC/P) microswimmers that provide biocompatibility, large specific surface area, and controllable movement. The results show that TC micromotors produced by physical mixing at a specific concentration exhibit a large specific surface area, reduced agglomeration, increased photocatalytic active sites, and improved photocatalytic stability. TC micromotors are effectively loaded into the porous hydrogel by 3D printing for multiscale fabrication to improve the photocatalytic performance across scales and realize effective and directional driving under violet light excitation. The TC/P microswimmers exhibit stable catalysis and motion in 7 days, at which point the catalysis by TiO2 is already ineffective. With stability, biocompatibility, and biomedical functions, this multiscale fabricated microswimmer exhibits great potential in micromanipulation and targeted therapy.

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Section: Resultsmentioning

confidence: 99%

3D‐Printed Light‐Driven Microswimmer with Built‐In Micromotors

Chen

Yang

et al. 2021

Adv Materials Technologies

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“…Finally, some of the fabrication strategies or stimuli used (heat, UV) can cause potential cell damage, which is often overlooked. [22,23,[26][27][28] Nature-derived hydrogels are often chosen as cell carriers due to their cytocompatibility, water storage or swelling capacity, and their ability to support tissue deposition. It is, therefore, key to find 4D strategies that are compatible with the current hydrogelbased bioink systems.…”

Section: Introductionmentioning

confidence: 99%

Swelling‐Dependent Shape‐Based Transformation of a Human Mesenchymal Stromal Cells‐Laden 4D Bioprinted Construct for Cartilage Tissue Engineering

Díaz-Payno

Kalogeropoulou

Muntz

et al. 2022

Adv Healthcare Materials

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3D bioprinting is usually implemented on flat surfaces, posing serious limitations in the fabrication of multilayered curved constructs. 4D bioprinting, combining 3D bioprinting with time-dependent stimuli-induced transformation, enables the fabrication of shape-changing constructs. Here, a 4D biofabrication method is reported for cartilage engineering based on the differential swelling of a smart multi-material system made from two hydrogel-based materials: hyaluronan and alginate. Two ink formulations are used: tyramine-functionalized hyaluronan (HAT, high-swelling) and alginate with HAT (AHAT, low-swelling). Both inks have similar elastic, shear-thinning, and printability behavior. The inks are 3D printed into a bilayered scaffold before triggering the shape-change by using liquid immersion as stimulus. In time (4D), the differential swelling between the two zones leads to the scaffold's self-bending. Different designs are made to tune the radius of curvature and shape. A bioprinted formulation of AHAT and human bone marrow cells demonstrates high cell viability. After 28 days in chondrogenic medium, the curvature is clearly present while cartilage-like matrix production is visible on histology. A proof-of-concept of the recently emerged technology of 4D bioprinting with a specific application for the design of curved structures potentially mimicking the curvature and multilayer cellular nature of native cartilage is demonstrated.

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“…Unlike hydrogel microbeads of unmodified Alg, high UV absorption of the composite microbeads between 250 and 290 nm by the phenol side chains [ 92 ] on Alg‐DSP, SF‐TA, and G‐TA provides a barrier against UV‐C and UV‐B radiation that can be utilized in chemical‐free sterilization [ 93 ] or photo‐crosslinking of hydrogels. [ 94 ] For example, 2‐hydroxy‐4‐(2‐hydroxyethoxy)‐2‐methylpropiophenone (Irgacure 2959), a cytocompatible photoinitiator that is widely used in photocrosslinking of cell encapsulating hydrogels, could not be utilized at its absorption peak of 260 nm due to DNA damage and apoptotic cell death from UV‐C radiation. [ 95 ] Instead, 365 nm is used to initiate photocrosslinking, which takes significantly longer than 260 nm due to lower molar extinction coefficient in the UV‐A region.…”

Section: Discussionmentioning

confidence: 99%

Cytoprotection of Human Progenitor and Stem Cells through Encapsulation in Alginate Templated, Dual Crosslinked Silk and Silk–Gelatin Composite Hydrogel Microbeads

Hasturk

Smiley

Arnett

et al. 2022

Adv Healthcare Materials

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Susceptibility of mammalian cells against harsh processing conditions limit their use in cell transplantation and tissue engineering applications. Besides modulation of the cell microenvironment, encapsulation of mammalian cells within hydrogel microbeads attract attention for cytoprotection through physical isolation of the encapsulated cells. The hydrogel formulations used for cell microencapsulation are largely dominated by ionically crosslinked alginate (Alg), which suffer from low structural stability under physiological culture conditions and poor cell–matrix interactions. Here the fabrication of Alg templated silk and silk/gelatin composite hydrogel microspheres with permanent or on‐demand cleavable enzymatic crosslinks using simple and cost‐effective centrifugation‐based droplet processing are demonstrated. The composite microbeads display structural stability under ion exchange conditions with improved mechanical properties compared to ionically crosslinked Alg microspheres. Human mesenchymal stem and neural progenitor cells are successfully encapsulated in the composite beads and protected against environmental factors, including exposure to polycations, extracellular acidosis, apoptotic cytokines, ultraviolet (UV) irradiation, anoikis, immune recognition, and particularly mechanical stress. The microbeads preserve viability, growth, and differentiation of encapsulated stem and progenitor cells after extrusion in viscous polyethylene oxide solution through a 27‐gauge fine needle, suggesting potential applications in injection‐based delivery and three‐dimensional bioprinting of mammalian cells with higher success rates.

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Irradiation with 365 nm and 405 nm wavelength shows differences in DNA damage of swine pancreatic islets

Cited by 31 publications

References 29 publications

3D‐Printed Light‐Driven Microswimmer with Built‐In Micromotors

3D‐Printed Light‐Driven Microswimmer with Built‐In Micromotors

Swelling‐Dependent Shape‐Based Transformation of a Human Mesenchymal Stromal Cells‐Laden 4D Bioprinted Construct for Cartilage Tissue Engineering

Cytoprotection of Human Progenitor and Stem Cells through Encapsulation in Alginate Templated, Dual Crosslinked Silk and Silk–Gelatin Composite Hydrogel Microbeads

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