Irrelevance between the Induction of Anti-Campylobacter Humoral Response by a Bacterin and the Lack of Protection against Homologous Challenge in Japanese Jidori Chickens

Okamura, Masashi; Tominaga, Ayumi; Ueda, Michihiro; Ohshima, Rumiko; Kobayashi, Masahide; Tsukada, Mari; Yokoyama, Eriko; Takehara, Kazuhiko; Deguchi, Kazuhiro; Honda, Takashi; Nakamura, Masayuki

doi:10.1292/jvms.11-0286

Cited by 6 publications

(5 citation statements)

References 16 publications

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“…The levels of log10 reduction from 15 of 20 trials were reported between 0.1 and 1.6 (mean or median) log10 reduction after homologous challenge. These trials were the crude lysate vaccines with OMP, OMP-NP orally with booster [ 47 ], DNA vaccine with purified FlaA cloned into pcDNA3 plasmids mixed with adjuvant subcutaneously or intramuscularly with booster [ 51 ], formalin-killed whole-cell vaccine mixed with oil adjuvants [ 52 ], subunit vaccine with 40 µg of recombinant NHC flagellin mixed adjuvant in ovo [ 53 ], and SE vectored vaccine expressing Omp18 protein (Cj0013), peptidoglycan associated lipoprotein of Salmonella (PAL of Salmonella ), and high mobility group box 1 protein (HMGB1) orally [ 58 ]. Five trial used a combination of DNA (prime) and subunit (boost) vaccine with four individual antigens and combinations of these antigens, delivered intramuscularly with booster upon heterologous challenge reported reduction levels ranging between 0.12 and 2.2 log10 CFU/g (using qPCR or bacterial culture methods) and between 1.06 and 1.92 log10 reductions in genome equivalents per gram (qPCR) [ 50 ].…”

Section: Resultsmentioning

confidence: 99%

“…Based on this review, 62 trials from 16 papers fulfilled the selection criteria and were included using C. jejuni loads in vaccinated broilers [ 24 , 25 , 26 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 ]. The variations of C. jejuni log10 reduction within these studies were estimated from different C. jejuni loads (log10) in ceca between vaccinated and non-vaccinated chickens [ 49 , 50 , 80 , 82 ].…”

Section: Discussionmentioning

confidence: 99%

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A Systematic Review of Campylobacter jejuni Vaccine Candidates for Chickens

et al. 2021

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Campylobacter jejuni infection linked to the consumption of contaminated poultry products is one of the leading causes of human enteric illness worldwide. Vaccination of chickens is one of the potential strategies that could be used to control C. jejuni colonization. To date, various C. jejuni vaccines using potential antigens have been evaluated, but a challenge in identifying the most effective formulation is the wide variability in vaccine efficacies reported. A systematic review was undertaken to compare C. jejuni vaccine studies. Based upon specific selection criteria eligible papers were identified and included in the analysis. Vaccine efficacy reported from different C. jejuni antigens, vaccine types, and vaccination regimens reported in these papers were reviewed. Our analysis shows that total outer membrane proteins and cysteine ABC transporter substrate-binding protein were among the most efficacious vaccine antigen candidates reported. This review also highlights the importance of the need for increased consistency in the way C. jejuni vaccine studies in poultry are designed and reported in order to be able to undertake a robust comparison of C. jejuni vaccine candidates.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Systematic Review of Campylobacter jejuni Vaccine Candidates for Chickens

et al. 2021

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“…Killed vaccines are relatively less effective in stimulating an immune response and require an adjuvant for vaccination. For instance, subcutaneous vaccination with formalin-killed C. jejuni using oil and aluminum hydroxide gel as adjuvants did not reduce the level of C. jejuni in chickens despite significant induction of anti- Campylobacter antibodies [ 25 ]. Oral vaccination with formalin-inactivated C. jejuni using Escherichia coli heat-labile toxin as an adjuvant increased the level of anti- C. jejuni secretory IgA but resulted in only a limited reduction in the C. jejuni level by 1.4 log CFU/g feces [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Live-Attenuated Oral Vaccines to Reduce Campylobacter Colonization in Poultry

et al. 2022

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The control of Campylobacter in poultry at the pre-harvest level is critical to reducing foodborne infections with Campylobacter since the consumption of contaminated poultry is the most frequent cause of human campylobacteriosis. Although poultry vaccination is suggested as useful intervention measures, no Campylobacter vaccines are currently available. To develop live-attenuated oral Campylobacter vaccines, in this study, we evaluated the efficacy of pre-colonization by oxidative stress defense mutants, including knockout mutants of ahpC, katA, and sodB, in preventing Campylobacter jejuni from colonizing poultry. Interestingly, when chickens were pre-colonized with ΔahpC and ΔkatA mutants, rather than the ΔsodB mutant, the level of C. jejuni colonization was significantly reduced within 35 days. Further studies demonstrated when chickens were pre-colonized with the ΔahpC mutant by oral challenge with a high dose (ca., 5 × 108 CFU/bird) and a low dose (ca., 5 × 106 CFU/bird), it twice reduced the level of C. jejuni by 3.9 log10CFU/g feces and 3 log10CFU/g feces after 42 days, respectively, compared to the untreated control. Due to a colonization defect, the ΔahpC mutant was removed from chickens within 42 days. After excretion from the host, moreover, the ΔahpC mutant cannot survive in aerobic environments because of compromised aerotolerance. Our findings suggest that the ahpC mutant has a great potential for on-farm application to control C. jejuni at the pre-harvest level.

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“…However, work is ongoing, and several candidate molecules have shown promise. Okamura et al (2012) immunised two groups of Jidori chicks subcutaneously with a formalin-killed C. jejuni with two different adjuvants. Both vaccines induced high levels of anti-Campylobacter IgG, but in challenge studies, the concentration of the organism in the caeca and faeces remained the same.…”

Section: Vaccinationmentioning

confidence: 99%

“…Bolton)Vaccination2 vaccines both formalin killed C. jejuni but in 2 different adjuvants (oil and aluminium hydroxide gel)Field‐based trial using Jidori chicks26 days post challenge:oil: 7.0 (1.2)Aluminium: 7.5 (0.7)Control: 7.0 (1.1)56 days post challenge:Oil: 6.8 (1.2)Aluminium: 6.6 (2.2)Control: 7.2 (1.1)17Okamura et al. ()Feed additiveMCFAApplied test conditions. Birds experimentally infected with C. jejuni at 15 days old and tested at 27 days oldControl: 7.0 (0.8)Tylosin 2.8 (1.5)Caproic acid 7.3 (0.8)Caprylic acid 7.8 (0.7)10Hermans et al.…”

Section: Appendix E – Studies On Effects Of Control Options On Broiler Farms To Reduce the Concentration Of Campylobacter In The Birdsmentioning

confidence: 99%

Update and review of control options for Campylobacter in broilers at primary production

Koutsoumanis¹,

Allende²,

Álvarez‐Ordóñez³

et al. 2020

EFS2

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The 2011 EFSA opinion on Campylobacter was updated using more recent scientific data. The relative risk reduction in EU human campylobacteriosis attributable to broiler meat was estimated for on‐farm control options using Population Attributable Fractions (PAF) for interventions that reduce Campylobacter flock prevalence, updating the modelling approach for interventions that reduce caecal concentrations and reviewing scientific literature. According to the PAF analyses calculated for six control options, the mean relative risk reductions that could be achieved by adoption of each of these six control options individually are estimated to be substantial but the width of the confidence intervals of all control options indicates a high degree of uncertainty in the specific risk reduction potentials. The updated model resulted in lower estimates of impact than the model used in the previous opinion. A 3‐log10 reduction in broiler caecal concentrations was estimated to reduce the relative EU risk of human campylobacteriosis attributable to broiler meat by 58% compared to an estimate larger than 90% in the previous opinion. Expert Knowledge Elicitation was used to rank control options, for weighting and integrating different evidence streams and assess uncertainties. Medians of the relative risk reductions of selected control options had largely overlapping probability intervals, so the rank order was uncertain: vaccination 27% (90% probability interval (PI) 4–74%); feed and water additives 24% (90% PI 4–60%); discontinued thinning 18% (90% PI 5–65%); employing few and well‐trained staff 16% (90% PI 5–45%); avoiding drinkers that allow standing water 15% (90% PI 4–53%); addition of disinfectants to drinking water 14% (90% PI 3–36%); hygienic anterooms 12% (90% PI 3–50%); designated tools per broiler house 7% (90% PI 1–18%). It is not possible to quantify the effects of combined control activities because the evidence‐derived estimates are inter‐dependent and there is a high level of uncertainty associated with each.

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Irrelevance between the Induction of Anti-Campylobacter Humoral Response by a Bacterin and the Lack of Protection against Homologous Challenge in Japanese Jidori Chickens

Cited by 6 publications

References 16 publications

A Systematic Review of Campylobacter jejuni Vaccine Candidates for Chickens

A Systematic Review of Campylobacter jejuni Vaccine Candidates for Chickens

Live-Attenuated Oral Vaccines to Reduce Campylobacter Colonization in Poultry

Update and review of control options for Campylobacter in broilers at primary production

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