Irreversible Binding of Chlorinated Ethylenes to Macromolecules

Abstract

JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org.. The National Institute of Environmental Health Sciences (NIEHS) andBrogan & Partners are collaborating with JSTOR to digitize, preserve and extend access to Environmental Health Perspectives. Rals have been eyosod in a dooed system to {be cll ethyEes Xyl d and ethylenfe and to t as a renoe _1. Data of e of tbe ||1|>b, of uy s i . _ ws . s v i i s s A ) ew qng anc r uc ear, soslonSwass. ana Ucarbon tetrachloride was purchased from the Radiochemical Centres Amersham, England. Exposure of RatsMale Wistar rats 200-250 gs were exposed in a closed all-glass system (volume 10.3 liters) to an atmosphere containing the 14C-labeled halogenated hydrocarbon. This system has been described elsewhere (13). Decline of radiactivity in the atmosphere of the system was followed by liquid scintillation counting (13). Concentration of haloge- December 1977 197

Degradation of chloroform by immobilized cells of Bacillus sp. in calcium alginate beads

“…No further degradation of chloroform was observed even after incubating for prolonged periods ([96 h), by nonimmobilized cells after the first cycle unlike the immobilized cells. Alvarez-Cohen and McCarty (1991) have speculated that phosgene and trichloroethylene epoxide are responsible for the product toxicities of chloroform and trichloroethylene, since both compounds exhibit irreversible binding to proteins thought to result in toxic behavior in mammalian system (Bolt and Filser 1977;Pohl et al 1977). A similar reaction may have caused the toxicity observed with this Bacillus sp.…”

Trichloroethylene — a Review of the Literature From a Health Effects Perspective

“…Additionally, the kidneys, spleen, and small intestineare involved in metabolism of TCE because these tissues are sites of cellular protein binding of metabolites from TCE (Bolt and Filser, 1977;Dalbey and Bingham, 1978;Bergman, 1983; Davidson and Beliles, 1991). Cytochrome P-450-mediated metabolism plays an important role in the toxicity of TCE (Ikeda and Ohtsuji, 1972;Bonse and Henschler, 1976;Henschler et al, 1979;Guengerich, 1982, 1983;Dekant et al, 1984;Prout et al, 1985;U.S.…”

Activities of chlorinated ethane and ethylene compounds in the Salmonella/rat microsome mutagenesis and rat hepatocyte/DNA repair assays under vapor phase exposure conditions

“…Synthetic epoxides of trichloroethylene and tetrachloroethylene have been shown to be genotoxic (Kline et al, 1982) and induce transformation in cultured hamster cells (DiPaolo and Doniger, 1982), but neither was carcinogenic by chronic skin application or subcutaneous injection in mice (Van Duuren et al, 1983). For several other compounds of this type, macromolecular binding, including to DNA, has been demonstrated in vitro (Van Duuren and Banerjee, 1976;Bolt and Filser, 1977;Laib et al, 1979;DiRenzo et al, 1982;MacDonald et al, 1982;Stott et al, 1982;Parchman and Magee, 1982;Miller and Guengerich, 1983). However, DNA binding of chlorinated ethanes and ethylenes other than monochloroethylene and l, !-dichloroethylene has been nonexistent or of a very low order (Schumann et al, 1980;Stott et al, 1982), and specific adducts have not been identified (Parchmann and Magee, 1982;Stott et al, 1982).…”