Irreversible electroporation (NanoKnife) in cancer treatment

Jourabchi, Natanel; Beroukhim, Kourosh; Tafti, Bashir Akhavan; Kee, Stephen T.; Lee, Edward W.

doi:10.1016/j.gii.2014.02.002

Cited by 80 publications

(42 citation statements)

References 85 publications

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“…The IRE procedure for liver tumors is standardly performed under general anesthesia as described in [16,22]. According to the current clinical recommendations, the interventional radiologist has to place percutaneously the needles in or around the tumor in a parallel configuration and at the same depth [12].…”

Section: Introductionmentioning

confidence: 99%

“…Roughly speaking, the interventional radiologists who perform IRE ablation of liver tumors place the needles as best as they can, and then the pulse delivery is performed. Several clinical studies have shown the clinical relevance of this approach [12,16,41,42]. However, the uncertainties of the needle positionning generate uncertainties on the electric field distribution, which may affect dramatically the treatment efficacy.…”

Section: Introductionmentioning

confidence: 99%

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Numerical workflow of irreversible electroporation for deep-seated tumor

Gallinato¹,

Séror²,

Poignard³

2019

Phys. Med. Biol.

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The paper provides a numerical workflow, based on the "real-life" clinical workflow of irreversible electroporation (IRE) performed for the treatment of deep-seated liver tumors. Thanks to a combination of numerical modeling, image registration algorithm and clinical data, our numerical workflow enables to provide the distribution of the electric field as effectively delivered by the clinical IRE procedure. As a proof of concept, we show on a specific clinical case of IRE ablation of liver tumor that clinical data could be advantageously combined to numerical simulations in a near future , in order to give to the interventional radiologists information on the effective IRE ablation. We also corroborate the simulated treated region with the post-treatment MRI performed 3 days after the treatment.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Numerical workflow of irreversible electroporation for deep-seated tumor

Gallinato¹,

Séror²,

Poignard³

2019

Phys. Med. Biol.

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“…A representative clinically used PEF pulse train consists of 80-100 square wave pulses each of width 100 ms delivered via electrodes into the tissue at a frequency of 1 Hz. Note that the clinical modality using PEFs is known as irreversible electroporation (IRE) or Nanoknife (2)(3)(4)(5)(6)(7). The delivery of energy via PEFs disrupts the plasma membrane, causing loss of cell homeostasis, which can lead to cell death if the cell does not recover from the perturbation.…”

Section: Introductionmentioning

confidence: 99%

Influence of Pulsed Electric Fields and Mitochondria-Cytoskeleton Interactions on Cell Respiration

Goswami

Perry

Allen

et al. 2018

Biophysical Journal

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Pulsed electric fields with microsecond pulse width (μsPEFs) are used clinically; namely, irreversible electroporation/Nanoknife is used for soft tissue tumor ablation. The μsPEF pulse parameters used in irreversible electroporation (0.5-1 kV/cm, 80-100 pulses, ∼100 μs each, 1 Hz frequency) may cause an internal field to develop within the cell because of the disruption of the outer cell membrane and subsequent penetration of the electric field. An internal field may disrupt voltage-sensitive mitochondria, although the research literature has been relatively unclear regarding whether such disruptions occur with μsPEFs. This investigation reports the influence of clinically used μsPEF parameters on mitochondrial respiration in live cells. Using a high-throughput Agilent Seahorse machine, it was observed that μsPEF exposure comprising 80 pulses with amplitudes of 600 or 700 V/cm did not alter mitochondrial respiration in 4T1 cells measured after overnight postexposure recovery. To record alterations in mitochondrial function immediately after μsPEF exposure, high-resolution respirometry was used to measure the electron transport chain state via responses to glutamate-malate and ADP and mitochondrial membrane potential via response to carbonyl cyanide-p-trifluoromethoxyphenylhydrazone. In addition to measuring immediate mitochondrial responses to μsPEF exposure, measurements were also made on cells permeabilized using digitonin and those with compromised cytoskeleton due to actin depolymerization via treatment with the drug latrunculin B. The former treatment was used as a control to tease out the effects of plasma membrane permeabilization, whereas the latter was used to investigate indirect effects on the mitochondria that may occur if μsPEFs impact the cytoskeleton on which the mitochondria are anchored. Based on the results, it was concluded that within the pulse parameters tested, μsPEFs alone do not hinder mitochondrial physiology but can be used to impact the mitochondria upon compromising the actin. Mitochondrial susceptibility to μsPEF after actin depolymerization provides, to our knowledge, a novel avenue for cancer therapeutics.

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“…Similar to radiotherapy treatment planning, conventional medical imaging modalities such as computed tomography, magnetic resonance imaging, and ultrasound imaging can be used for pre-procedural and peri-procedural treatment planning to determine effective tissue volume ablation (Jourabchi et al, 2014). The delivered electric pulse train can be tailored to each patient by modifying the number of pulses (exposure time), the electric field strength (ratio of applied voltage to electrode distance), and the interval between pulses (to synchronize with cardiac rhythm), such that critical features (blood vessels and extracellular matrix) can be spared (Edd et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Irreversible electroporation inhibits pro-cancer inflammatory signaling in triple negative breast cancer cells

Goswami

Coutermarsh-Ott

Morrison

et al. 2017

Bioelectrochemistry

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Low-level electric fields have been demonstrated to induce spatial re-distribution of cell membrane receptors when applied for minutes or hours. However, there is limited literature on the influence on cell signaling with short transient high-amplitude pulses typically used in irreversible electroporation (IRE) for cancer treatment. Moreover, literature on signaling pertaining to immune cell trafficking after IRE is conflicting. We hypothesized that pulse parameters (field strength and exposure time) influence cell signaling and subsequently impact immune-cell trafficking. This hypothesis was tested in-vitro on triple negative breast cancer cells treated with IRE, where the effects of pulse parameters on key cell signaling factors were investigated. Importantly, real time PCR mRNA measurements and ELISA protein analyses revealed that thymic stromal lymphopoietin (TSLP) signaling was down regulated by electric field strengths above a critical threshold, irrespective of exposure times spanning those typically used clinically. Comparison with other treatments (thermal shock, chemical poration, kinase inhibitors) revealed that IRE has a unique effect on TSLP. Because TSLP signaling has been demonstrated to drive pro-cancerous immune cell phenotypes in breast and pancreatic cancers, our finding motivates further investigation into the potential use of IRE for induction of an anti-tumor immune response in vivo.

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Irreversible electroporation (NanoKnife) in cancer treatment

Cited by 80 publications

References 85 publications

Numerical workflow of irreversible electroporation for deep-seated tumor

Numerical workflow of irreversible electroporation for deep-seated tumor

Influence of Pulsed Electric Fields and Mitochondria-Cytoskeleton Interactions on Cell Respiration

Irreversible electroporation inhibits pro-cancer inflammatory signaling in triple negative breast cancer cells

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