Several studies suggest that optimum N rate and application frequency differ among citrus rootstocks. 'Rhode Red' valencia orange trees [Citrus sinensis (L.) Osb.] on three rootstocks, C. volkameriana Ten. and Pasq., 'Carrizo' citrange [C. sinensis (L.) Osb. x Poncirus trifoliata (L.) Raf.], and 'Swingle' citrumelo [C. paradisi Macf. x P. trifoliata (L.) Raf.], were used to determine if N rate and application frequency should be adjusted, based on rootstock, during the first 3 years in the field. Treatments were arranged in a 3 × × × × × 3 × × × × × 3 (rootstock, N rates, N application frequency, respectively) factorial experiment. Annual N application rates ranged from 68 to 272 g/tree depending on tree age, and N was applied biweekly, weekly or monthly. Application frequency had no effect on trunk diameter or leaf N concentration in any year. Rootstock had a significant effect on growth in all 3 years, with trees on C. volkameriana being largest and having the greatest yields, followed by those on 'Carrizo' and 'Swingle', respectively. Trees on C. volkameriana were larger than those on the other rootstocks because they were larger at planting, grew over a longer period during the year, and often grew at a faster rate. Nitrogen rate had no effect on growth during the first 2 years in the field, but the highest N rate increased yields in year 3 for trees on C. volkameriana and 'Swingle' rootstocks. Interaction between rootstock and N rate was nonsignificant for trunk diameter, but it was significant for yield, suggesting that trees on C. volkameriana responded more to increased N than did those on the other rootstocks.