Objective: the aim of this study was to establish the profile of dentists with respect to some characteristics in the use of systems and kinematics of instrumentation most used in Brazil for endodontic treatment. Material and Methods: an invitation to participate in the survey was sent digitally to 1,300 dentists from January-April 2016, distributed in the five regions of Brazil, using a service available at surveymonkey.com (Survey Monkey, Palo Alto, CA, USA). They were validated 1,143 with the option of free and informed consent. The data collected were divided into two groups, the first group being only general practioners group (CG Group) and the second group, dentists that did postgraduation in Endodontics (PE Group). Data were statistically analyzed using the Mann-Whitney and Likelihood Ratio (p <0.05). Results: most dentists of the two groups reside in the Southeast region; Are of the female gender; Average age between 26 and 34 years and have a postgraduate degree. The most striking feature of the use of the reciprocating system was the reduction in working time. Conclusion: within the limitations of the present research, it was concluded that most dentists presented a similar profile regardless of whether or not they had a postgraduate course, since they do not use rotating or reciprocating systems exclusively, being Protaper Universal® and Reciproc® the most used, associate the systems with manual files and reuse the files of the systems.