Eye tracking technology has become increasingly prevalent in scientific research, offering unique insights into oculomotor and cognitive processes. The present article explores the relationship between scientific theory, the research question, and the use of eye-tracking technology. It aims to guide readers in determining if eye tracking is suitable for their studies and how to formulate relevant research questions. Examples from research on oculomotor control, reading, scene perception, task execution, visual expertise, and instructional design are used to illustrate the connection between theory and eye-tracking data. These examples may serve as inspiration to researchers new to eye tracking. In summarizing the examples, three important considerations emerge: (1) whether the study focuses on describing eye movements or uses them as a proxy for e.g., perceptual, or cognitive processes, (2) the logical chain from theory to predictions, and (3) whether the study is of an observational or idea-testing nature. We provide a generic scheme and a set of specific questions that may help researchers formulate and explicate their research question using eye tracking.