Is canine calprotectin in serum stabile after storage at low temperature?

Kostanjšak, Tara; Bojanić, Krunoslav; Čičak, Helena; Gotić, Jelena; Vrbanac, Zoran; Šimundić, Ana-Maria; Vnuk, Dražen; Bottegaro, Nika Brkljača

doi:10.1186/s12917-022-03534-8

Cited by 4 publications

(3 citation statements)

References 24 publications

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“…More studies are required to investigate the stability of CLP under − 80 °C for 6 months-the storage conditions recommended by our ELISA Kit-Bioassay Technology Laboratory Human CLP ELISA Kit [60]. In support of our suggestion regarding the impact of storage duration on serum CLP stability, a recently published study in 2022 assessed serum canine CLP and found that it is unstable at 16 weeks of storage at − 80 °C and concluded a safe storage duration of 8 weeks [61]. Another important pre-analytical factor that influences the level of CLP is the presence of anticoagulants [62,63].…”

Section: Discussionmentioning

confidence: 79%

Study of serum calprotectin level in rheumatoid arthritis: unexpected low level and possible explanations

El-Tawab,

Moharram,

Younis

et al. 2024

Egypt Rheumatol Rehabil

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Background Assessment of disease activity in rheumatoid arthritis (RA) is crucial to optimize the response to treatment and prevent radiographic progression. DAS28 is the most commonly used disease activity index, which incorporates either erythrocyte sedimentation rate (ESR) or C-reactive protein (CRP). Several studies showed that using ESR and/or CRP in assessing disease activity falls short of detecting a significant portion of patients with active disease. Calprotectin (CLP) is an interesting protein that was found to be a promising biomarker of disease activity in RA patients’ sera when CRP is normal. This study aimed to measure serum CLP level in 50 RA patients with different grades of disease activity and compare its level with age- and sex-matched control. Results In this case–control study, the mean serum CLP level was significantly lower in RA patients (25.94 ± 25.87 ng/ml) compared to the control group values (53.02 ± 77.93 ng/ml), p < 0.001. The measured serum CLP in RA patients was lower than its level in other published studies. No significant difference was found between patients with different disease activity grades in the serum CLP level (H = 4.28, p = 0.23). Serum samples were collected and stored from RA patients over 4 months and from the control subjects over 1.5 months and were stored at –80 °C until analysis was performed according to the manufacturer’s instruction. Conclusion The low level of serum CLP among RA patients is most probably due to proteolysis related to storage conditions. Pre-analytic factors like the type of blood sample, whether the sample is fresh or frozen, and duration of storage exert an effect on serum CLP level when measured by enzyme-linked immunosorbent assay.

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Section: Discussionmentioning

confidence: 79%

Study of serum calprotectin level in rheumatoid arthritis: unexpected low level and possible explanations

El-Tawab,

Moharram,

Younis

et al. 2024

Egypt Rheumatol Rehabil

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“…Additionally, 10 mL of venous blood was collected from the forelimbs of the beagles. Serum was collected by centrifugation at 4°C, 464× g (1,800 rpm, Centrifuge 5,702, Eppendorf, Germany) for 10 min ( Kostanjšak et al, 2022 ; Zentrichová et al, 2023 ).…”

Section: Methodsmentioning

confidence: 99%

Impact of Pediococcus acidilactici GLP06 supplementation on gut microbes and metabolites in adult beagles: a comparative analysis

Zhao,

Zhang,

et al. 2024

Front. Microbiol.

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There is growing interest in the potential health benefits of probiotics for both humans and animals. The study aimed to investigate the effects of feeding the canine-derived probiotic Pediococcus acidilactici GLP06 to adult beagles by analysing the microbiome and metabolome. Twenty-four healthy adult beagles were randomly assigned to four groups. The CK group received a standard diet, while the three probiotic groups, the LG group (2 × 108 CFU/day/dog), MG group (2 × 109 CFU/day/dog), and HG group (2 × 1010 CFU/day/dog), received the standard diet supplemented with varying amounts of probiotics. The results show that, compared to the CK group, total antioxidant capacity was significantly increased in the MG and HG groups (p < 0.05), and superoxide dismutase and catalase were significantly increased in the HG group (p < 0.05). Compared to the CK group, malondialdehyde and blood urea nitrogen content were significantly decreased in the MG and HG groups (p < 0.05). Additionally, secretory immunoglobulin A activity was significantly increased in the HG group compared to the CK and LG groups (p < 0.05), and immunoglobulin G activity was significantly increased in the HG group compared to the CK, LG, and MG groups (p < 0.05). In addition, compared with the CK group, the abundance of Faecalitalea and Collinsella increased in the LG group, and the relative abundance of Tyzzerella and Parasutterella increased in the MG group. The α diversity and the relative abundances of beneficial bacteria (Faecalibacterium, Lachnospiraceae_NK4A1316, and Ruminococcaceae_UCG-005) were higher in the HG group than in the CK group. Furthermore, acetic acid content was significantly increased in the HG group compared to the CK, LG, and MG groups (p < 0.05). Butyric acid, isobutyric acid, and the total SCFA content were significantly increased in the HG group compared to the CK group (p < 0.05). Moreover, metabolome analysis revealed 111 upregulated and 171 downregulated metabolites in the HG group. In conclusion, this study presents evidence that supplementing with P. acidilactici GLP06 can have a positive impact on antioxidant activity, immunoproteins, SCFAs, and gut microbiota in adult beagles. These findings highlight the potential of probiotics as a dietary intervention to enhance gut health and overall wellbeing in companion animals.

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“…For instance, donkey serum has been demonstrated to remain stable when stored at − 80 °C for up to a year 16 . However, the concentration of calreticulin protein shows a significant shift after being stored at − 80 °C for more than 8 weeks, with a marked increase observed after 16 weeks 20 . Ferritin showcases impressive stability, with a consistent shelf life of 3–5 years when stored at − 80 °C 21 .…”

Section: Introductionmentioning

confidence: 99%

Impact of ultra-low temperature storage on serum sIgE detection and allergic disease biobank feasibility

Chang,

Hu,

Pan

et al. 2023

Sci Rep

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Research has shown that the concentration and composition of biological samples may change after long-term ultra-low temperature storage. Consequently, this study examined the effect of ultra-low temperature storage on serum sIgE detection by comparing sIgE concentrations at various durations from the time of sample storage to subsequent testing. We selected 40 serum samples from the Guangzhou Medical University Affiliated First Hospital Biobank, which had been tested for house dust mites, dog hair, tobacco mold, cockroaches, and cow milk allergen sIgE. Samples were categorized by storage duration: 14 samples stored for 10 years, 12 for 5 years, and 14 for 3 years. They were also classified by sIgE positive levels: 15 samples at levels 1–2, 15 at levels 3–4, and 10 at levels 5–6. The allergen sIgE of these samples was retested using the same technology. Regardless of the type of allergen or the level of positivity, the majority of sIgE concentrations measured at the time of storage were higher than the current measurements, but the difference was not statistically significant. The correlation between the sIgE results at the time of storage and the current results was high for samples stored for 10 years (rs = 0.991, P < 0.001) and 5 years (rs = 0.964, P < 0.001). Serum allergen sIgE is stable when stored under ultra-low temperature conditions, making the construction of a biological sample bank for allergic diseases feasible. This will facilitate researchers in quickly obtaining samples, conducting technical research, and translating findings, thereby promoting the development of the field of allergy through integration of industry, academia, and research.

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Is canine calprotectin in serum stabile after storage at low temperature?

Cited by 4 publications

References 24 publications

Study of serum calprotectin level in rheumatoid arthritis: unexpected low level and possible explanations

Study of serum calprotectin level in rheumatoid arthritis: unexpected low level and possible explanations

Impact of Pediococcus acidilactici GLP06 supplementation on gut microbes and metabolites in adult beagles: a comparative analysis

Impact of ultra-low temperature storage on serum sIgE detection and allergic disease biobank feasibility

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