Is cyclic guanosine 3',5'-monophosphate a cell cycle regulator?

Abstract

Transient increases in the intracellular level of cyclic guanosine 3',5'-monophosphate have been observed at a periodicity of one generation time in two spoT strains of Escherichia coli and in Bacillus lichenifornis.

Cyclic nucleotides in procaryotes.

“…Bernlohr et al (22), using a complex enzyme cycling assay, found cGMP to be present in concentrations as high as 30 nM in E. coli. With a more conventional radioimmune assay, steady-state intracellular concentrations of cGMP of 0.5 to 3 nM were found (40). In other experiments, these authors obtained synchronous division by starving cells for various nutrients.…”

“…Cook et al (40) observed a sharp increase in cGMP levels in B. licheniformis undergoing synchronous division with a periodicity equivalent to one generation. They proposed that cGMP could be involved in some aspect of regulation of cellular division.…”

Synthesis of the cell surface during the division cycle of rod-shaped, gram-negative bacteria.

“…For example, a sudden change in the cytoplasmic calcium concentration has been proposed as a signal for a number of cell cycle events (76,123). Cyclic nucleotides have also been suggested as being involved in cell cycle regulation (25). The evidence for division cycle variations in the concentrations or amounts of various cytoplasmic substances have been considered.…”

Mechanism of CRP-mediated cya suppression in Escherichia coli

“…A class of mutants has been described recently which contains a cya mutation and yet expresses many, if not all, operons requiring the cAMP-CRP complex for positive control (1,5,6,21,26). Genetic analysis showed that these cya suppressor strains contain a second-site mutation that lies in the crp gene (1,5,6,21,26). Takebe et al (26) provided biochemical evidence that the cya suppressor mutation resulted in the production of an altered CRP.…”