Is dexamethasone-induced muscle atrophy an alternative model for naturally aged sarcopenia model?

Wang, Belle Yu-Hsuan; Hsiao, Allen Wei-Ting; Wong, Nicodemus; Chen, Yifan; Lee, Chien‐Wei; Lee, Wayne

doi:10.1016/j.jot.2022.11.005

Cited by 21 publications

(7 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Skeletal muscle protein degradation is regulated by muscle-specific E3 ubiquitin ligases, such as Muscle RING finger 1 (MuRF1) and muscle atrophy F-Box (atrogin-1/MAFbx) [ 46 ]. MuRF1 and atrogin-1 inhibit the PI3K-Akt signaling pathway and protein synthesis by downregulating mTOR expression [ 30 ]. In particular, MuRF1 is involved in the degradation of muscle fiber proteins, such as actin and MHC, and atrogin-1 plays a role in regulating protein synthesis [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, an increase in reactive oxygen species (ROS) by DEX treatment promotes the ubiquitination of muscle proteins by increasing the expression of E3 ligase and muscle atrophy caused by mitochondrial dysfunction [ 29 ]. Consequently, several studies have used dexamethasone to induce muscle atrophy both in vitro and in vivo [ 30 - 32 ]. Accordingly, we also evaluated the muscle improvement effect of L. rhamnosus JY02 in C2C12 muscle cells and C57BL/6 mice using DEX as a muscle atrophy inducer.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lactobacillus rhamnosus JY02 Ameliorates Sarcopenia by Anti-Atrophic Effects in a Dexamethasone-Induced Cellular and Murine Model

Lee

Kang

Yoo

et al. 2023

J. Microbiol. Biotechnol.

View full text Add to dashboard Cite

Sarcopenia is defined as loss of muscle mass and strength due to aging. Recent studies show that sarcopenia may improve via the gut–muscle axis, suggesting that gut health may affect muscle phenotypes. In this study, we aimed to investigate the ability of Lactobacillus rhamnosus JY02 as a probiotic strain isolated from kimchi to alleviate sarcopenia. L. rhamnosus JY02-conditioned medium (CM) reduced dexamethasone (DEX)-induced myotube diameter atrophy and expression of muscle degradation markers (MuRF1 and atrogin-1) in C2C12 cells. The amelioration of sarcopenia was investigated by measuring body composition (lean mass), hand grip strength, myofibril size (using histological analysis), and mRNA and protein expression of muscle-related factors in a DEX-induced mouse model. The results of these analyses showed that L. rhamnosus JY02 supplementation promoted the production of muscle-enhancement markers (MHC Iβ, MHC IIα, and Myo-D) and reduced both the production of muscle degradation markers and the symptoms of muscle atrophy (loss of lean mass and muscle strength). We also found decreased levels of pro-inflammatory cytokines (IL-6, IFN-γ) and increased levels of anti-inflammatory cytokines (IL-10) in the serum of DEX+JY02-administered mice compared to those in DEX-treated mice. Overall, these results suggest that L. rhamnosus JY02 is a potent probiotic supplement that prevents sarcopenia by suppressing muscle atrophy.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lactobacillus rhamnosus JY02 Ameliorates Sarcopenia by Anti-Atrophic Effects in a Dexamethasone-Induced Cellular and Murine Model

Lee

Kang

Yoo

et al. 2023

J. Microbiol. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Consequently, increasing socioeconomic burdens on the healthcare system are detrimental to society. As a result, a growing array of studies on sarcopenia, adopting animal models to investigate novel therapeutic approaches to delay its impact 46 , are being conducted.…”

Section: Discussionmentioning

confidence: 99%

Comparing the efficacy of concomitant treatment of resistance exercise and creatine monohydrate versus multiple individual therapies in age related sarcopenia

Elgizawy,

Amer,

Ali

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Aging-related sarcopenia is a degenerative loss of strength and skeletal muscle mass that impairs quality of life. Evaluating NUDT3 gene and myogenin expression as new diagnostic tools in sarcopenia. Also, comparing the concomitant treatment of resistance exercise (EX) and creatine monohydrate (CrM) versus single therapy by EX, coenzyme Q10 (CoQ10), and CrM using aged rats. Sixty male rats were equally divided into groups. The control group, aging group, EX-treated group, the CoQ10 group were administered (500 mg/kg) of CoQ10, the CrM group supplied (0.3 mg/kg of CrM), and a group of CrM concomitant with resistance exercise. Serum lipid profiles, certain antioxidant markers, electromyography (EMG), nudix hydrolase 3 (NUDT3) expression, creatine kinase (CK), and sarcopenic index markers were measured after 12 weeks. The gastrocnemius muscle was stained with hematoxylin–eosin (H&E) and myogenin. The EX-CrM combination showed significant improvement in serum lipid profile, antioxidant markers, EMG, NUDT3 gene, myogenin expression, CK, and sarcopenic index markers from other groups. The NUDT3 gene and myogenin expression have proven efficient as diagnostic tools for sarcopenia. Concomitant treatment of CrM and EX is preferable to individual therapy because it reduces inflammation, improves the lipid serum profile, promotes muscle regeneration, and thus has the potential to improve sarcopenia.

show abstract

“…The high-dose Dex-induced muscle atrophy model disrupts the balance between protein synthesis and degradation, leading to muscle fiber damage and atrophy [ 33 ]. This model is widely utilized, as it allows the easy and efficient reproduction of naturally occurring muscle wasting conditions, regardless of in vitro or in vivo models [ 34 ]. Dex treatment in zebrafish has been reported to significantly induce muscular atrophy, leading to decreased swimming endurance and impaired motor performance [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Exploring the Potential of Olive Flounder Processing By-Products as a Source of Functional Ingredients for Muscle Enhancement

Hyun,

Kang,

Lee

et al. 2023

Antioxidants

View full text Add to dashboard Cite

Olive flounder (OF) is a widely aqua-cultivated and recognized socioeconomic resource in Korea. However, more than 50% of by-products are generated when processing one OF, and there is no proper way to utilize them. With rising awareness and interest in eco-friendly bio-materialization recycling, this research investigates the potential of enzymatic hydrolysis of OF by-products (OFB) to produce functional ingredients. Various enzymatic hydrolysates of OFB (OFBEs) were generated using 11 commercial enzymes. Among them, Prozyme 2000P-assisted OFBE (OFBP) exhibited the highest protein content and yield, as well as low molecularization. The muscle regenerative potential of OFBEs was evaluated using C2C12 myoblasts, revealing that OFBP positively regulated myoblast differentiation. In an in vitro Dex-induced myotube atrophy model, OFBP protected against muscle atrophy and restored myotube differentiation and Dex-induced reactive oxygen species (ROS) production. Furthermore, zebrafish treated with OFBEs showed improved locomotor activity and body weight, with OFBP exhibiting outstanding restoration in the Dex-induced muscle atrophy zebrafish in vivo model. In conclusion, OFBEs, particularly OFBP, produce hydrolysates with enhanced physiological usability and muscle regenerative potential. Further research on its industrial application and mechanistic insights is needed to realize its potential as a high-quality protein food ingredient derived from OF processing by-products.

show abstract

Is dexamethasone-induced muscle atrophy an alternative model for naturally aged sarcopenia model?

Cited by 21 publications

References 70 publications

Lactobacillus rhamnosus JY02 Ameliorates Sarcopenia by Anti-Atrophic Effects in a Dexamethasone-Induced Cellular and Murine Model

Lactobacillus rhamnosus JY02 Ameliorates Sarcopenia by Anti-Atrophic Effects in a Dexamethasone-Induced Cellular and Murine Model

Comparing the efficacy of concomitant treatment of resistance exercise and creatine monohydrate versus multiple individual therapies in age related sarcopenia

Exploring the Potential of Olive Flounder Processing By-Products as a Source of Functional Ingredients for Muscle Enhancement

Contact Info

Product

Resources

About