Is Drosophila suzukii as susceptible to entomopathogenic nematodes as Drosophila melanogaster?

Garriga, Anna; Morton, Ana; Pino, Fernando García del

doi:10.1007/s10340-017-0920-6

Cited by 29 publications

(19 citation statements)

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“…Poyet et al [33] described a strong immune reaction against the parasitoid L. heterotoma, which leads to the encapsulation of wasp eggs. Even if D. suzukii larvae and adults are susceptible to nematodes [36], no immunological studies on the relationships between these pests and EPNs have been made before; consequently, we evaluated humoral and cellular responses upon infection of S. carpocapsae and X. nematophila.…”

Section: Discussionmentioning

confidence: 99%

“…Studies with larvae of D. suzukii showed a strong immune response of encapsulation to parasitoid eggs of Leptopilina heterotoma Thompson (Hymenoptera: Figitidae) that discourages their use for controlling the pest [32,33]. Instead, pupal parasitoids, entomopathogenic fungi, and EPNs achieved better results controlling the fly under laboratory conditions [34][35][36][37]. Susceptibility of D. suzukii larvae was evaluated against different EPN species, as S. carpocapsae, Steinernema feltiae (Filipjev) (Rhabditida: Steinernematidae), and Heterorhabditis bacteriophora (Poinar) (Rhabditida: Heterorhabditidae) [36].…”

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confidence: 99%

“…Instead, pupal parasitoids, entomopathogenic fungi, and EPNs achieved better results controlling the fly under laboratory conditions [34][35][36][37]. Susceptibility of D. suzukii larvae was evaluated against different EPN species, as S. carpocapsae, Steinernema feltiae (Filipjev) (Rhabditida: Steinernematidae), and Heterorhabditis bacteriophora (Poinar) (Rhabditida: Heterorhabditidae) [36]. This study reported a high susceptibility of the fly after nematodes killed the larvae and reproduced inside them.…”

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confidence: 99%

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Immune Response of Drosophila suzukii Larvae to Infection with the Nematobacterial Complex Steinernema carpocapsae–Xenorhabdus nematophila

Garriga

Mastore

Morton

et al. 2020

Insects

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Entomopathogenic nematodes have been proposed as biological agents for the control of Drosophila suzukii, an invasive pest of small-stone and soft-skinned fruits. Larvae of the fly are susceptible to Steinernema carpocapsae infection but the reaction of immune defenses of the host are unknown. To determine the immune response, larvae were infected with S. carpocapsae and Xenorhabdus nematophila to evaluate the effector mechanisms of both humoral and cellular processes. The symbiont bacteria presented an inhibitory effect on the phenoloxidase cascade with a low level of melanization. Besides, X. nematophila activated the synthesis of putative antimicrobial peptides on the hemolymph of infected larvae. However, those peptides presented a lower antimicrobial activity compared to hemolymph from larvae infected with non-symbiont bacteria. Xenorhabdus nematophila avoided also the phagocytosis response of hemocytes. During in vitro and in vivo assays, S. carpocapsae was not encapsulated by cells, unless the cuticle was damaged with a lipase-treatment. Hemocyte counts confirmed differentiation of lamellocytes in the early phase of infection despite the unrecognition of the nematodes. Both X. nematophila and S. carpocapsae avoided the cellular defenses of D. suzukii larvae and depressed the humoral response. These results confirmed the potential of entomopathogenic nematodes to control D. suzukii.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Immune Response of Drosophila suzukii Larvae to Infection with the Nematobacterial Complex Steinernema carpocapsae–Xenorhabdus nematophila

Garriga

Mastore

Morton

et al. 2020

Insects

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“…Kamali et al (2013) explained reasοns fοr high incidence οf infectiοn οf larνae cοmpared tο pupae and adults are due tο their deνelοpmental duratiοn, actiνity in sοil, οutput οf cues related tο hοst finding by EPNs and larger natural οpenings. Fοr example, the lοw susceptibility οf pupae, which is highly οbserνed in οther studies (Hübner et al 2017) can be attributed tο lack οf natural pathway entry fοr nematοdes, as well as a mοre tοugh cuticle (Garriga et al 2018). In fact, the large natural οpenings οf the bοdy οf the larνae and the weakly sclerοtized larνal integument (relatiνe tο the nymphal integument) facilitate infectiοn by the nematοde.…”

Section: Susceptibility οF C Capitata Pupaementioning

confidence: 99%

Efficacy of Turkish isolate of Steinernema feltiae (Rhabditida: Steinernematidae) in controlling the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), under laboratory conditions

Chergui

Benzehra

Boudjemaa

et al. 2019

Egypt J Biol Pest Control

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The Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), is one of the mοst destructiνe pests in fruit grοwings. It pupates in the soil. The pupae are target οf many οrganisms sheltering the sοil such as the entοmοpathοgenic nematοdes (EPN). Pathοgenicity οf the Turkish strain οf the EPN, Steinernema feltiae, was eνaluated against late instar larνae, pupae, and adults οf C. capitata under labοratοry conditions. Suspensiοns οf the nematοde were applied at four increasing cοncentratiοns of (0 (fοr cοntrοl) 50, 100, and 200 IJs/ml) in 1 ml οf distilled water. The infectiνity οf S. feltiae against sοil stages οf C. capitata under different sοil mοisture leνels of 100, 75, and 50% οf field capacity was evaluated. Mοrtality rates were recοrded after 5 days οf treatment. In οrder tο cοnfirm the nematοde infectiοn, the dead larνae and pupae were cοllected and incubated until the appearance οf the infectiοus juνenile (IJs) οr dissected under a stereomicrοscοpe tο check for nematοdes. The last instar larνae and newly fοrmed pupae were mοre susceptible tο EPN infectiοn than οld pupae. The infectiνity was directly prοpοrtiοnal to the increase of soil moisture. The highest mοrtality (75%) was οbtained. S. feltiae was able tο infect adults easily because οf the multiple ways οf entrance for the nematodes (mοuth, anus, and spiracles) than the larvae and/or pupae. Therefore, the Turkish isοlate οf S. feltiae cοuld be useful fοr an integrated pest management prοgram οf C. capitata.

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“…Recent research effort has focused on management strategies to minimise further spread of D. suzukii and associated economic losses, with a focus on detection, tracking and trap and bait design (Landolt et al 2012;Feng et al 2018;Frewin et al 2017;Huang et al 2017), biological control (e.g. Becher et al 2017;Garriga et al 2018;Giorgini et al 2018;Girod et al 2018;Woltz et al 2015), harvesting techniques (Leach et al 2018) and the development of biopesticides (Fanning et al 2018). However, current management programs still rely heavily on broad-spectrum insecticides (Desneux et al 2007;Diepenbrock et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Desiccation, thermal stress and associated mortality in Drosophila fruit flies induced by neuropeptide analogue treatment

et al. 2019

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Drosophila suzukii is a serious pest of soft fruit worldwide. With the global over-dependence on broad-spectrum pesticides, a strong imperative exists for more environmentally friendly and targeted methods of control. One promising avenue involves employing synthetic neuropeptide analogues as insecticidal agents to reduce pest fitness. Neuropeptides, central to the regulation of physiological and behavioural processes, play a vital role in cold and desiccation survival. Building upon this, the current study investigated the effects of biostable kinin, the cardioacceleratory peptide CAP2b and pyrokinin (PK) analogues (the latter of which have previously displayed cross-talk with the capa receptor), on desiccation, starvation and cold stress tolerance of the pest, D. suzukii, and the closely related non-pest, D. melanogaster. Results demonstrated analogues of the superfamily (CAP2b and PK derived) significantly impacted survival of the target insect under conditions of desiccation stress. However, these peptides enhanced desiccation stress survival in relation to controls, suggesting that they may act as antagonists of the capa signalling pathway in the Malpighian tubules. Of particular note was the ability of analogues 1895 (2Abf-Suc-FGPRLa) and 1902 (2Abf-Suc-FKPRLa) to impact D. suzukii but not D. melanogaster. A focus on native Drosophila CAP2b/PK and kinin sequences in analogue development may yield pure agonists with diuretic action that may reduce desiccation stress survival in the pest flies. In highlighting the PRXamide neuropeptide superfamily more generally, and the structures of promising analogues more specifically, this research will feed the evolution of next-generation analogues and drive forward the development of neuropeptidomimetic-based agents. Keywords CAP2b • Dromekinin • Drosophila suzukii • G-protein-coupled receptors • Insecticide • Peptidomimetics • Pyrokinin Key message • The employment of synthetic neuropeptide analogues represents a novel approach to target-specific and environmentally friendly pest control. • Biostable kinin, CAP2b and pyrokinin (PK) analogues were developed and tested ex vivo in Malpighian tubule fluid secretion assays. • Analogues were subsequently tested in vivo for an ability to reduce the fitness of a pest species (Drosophila suzukii) under a variety of stress conditions relative to a non-pest species (Drosophila melanogaster). • Results highlight the structure of neuropeptides of the PRXamide superfamily as candidates in the drive for neuropeptidomimetic-based insecticidal agents.

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Is Drosophila suzukii as susceptible to entomopathogenic nematodes as Drosophila melanogaster?

Cited by 29 publications

References 41 publications

Immune Response of Drosophila suzukii Larvae to Infection with the Nematobacterial Complex Steinernema carpocapsae–Xenorhabdus nematophila

Immune Response of Drosophila suzukii Larvae to Infection with the Nematobacterial Complex Steinernema carpocapsae–Xenorhabdus nematophila

Efficacy of Turkish isolate of Steinernema feltiae (Rhabditida: Steinernematidae) in controlling the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), under laboratory conditions

Desiccation, thermal stress and associated mortality in Drosophila fruit flies induced by neuropeptide analogue treatment

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