Is Fe3+/2+ cycling an intermediate in sulphur oxidation by Fe2+-grown Thiobacillus ferrooxidans

Corbett, C

doi:10.1016/0378-1097(87)90273-4

Cited by 18 publications

(28 citation statements)

References 5 publications

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“…A proportion of the electrons generated from the anaerobic disproportionation of S 0 could be transported by reverse electron flow to the NADH dehydrogenase. The energy required for this process could come from reverse proton motive force (24,52).…”

Section: Resultsmentioning

confidence: 99%

Anaerobic Sulfur Metabolism Coupled to Dissimilatory Iron Reduction in the Extremophile Acidithiobacillus ferrooxidans

Osório

Mangold

Denis

et al. 2013

Appl Environ Microbiol

137

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Gene transcription (microarrays) and protein levels (proteomics) were compared in cultures of the acidophilic chemolithotroph Acidithiobacillus ferrooxidans grown on elemental sulfur as the electron donor under aerobic and anaerobic conditions, using either molecular oxygen or ferric iron as the electron acceptor, respectively. No evidence supporting the role of either tetrathionate hydrolase or arsenic reductase in mediating the transfer of electrons to ferric iron (as suggested by previous studies) was obtained. In addition, no novel ferric iron reductase was identified. However, data suggested that sulfur was disproportionated under anaerobic conditions, forming hydrogen sulfide via sulfur reductase and sulfate via heterodisulfide reductase and ATP sulfurylase. Supporting physiological evidence for H 2 S production came from the observation that soluble Cu 2؉ included in anaerobically incubated cultures was precipitated (seemingly as CuS). Since H 2 S reduces ferric iron to ferrous in acidic medium, its production under anaerobic conditions indicates that anaerobic iron reduction is mediated, at least in part, by an indirect mechanism. Evidence was obtained for an alternative model implicating the transfer of electrons from S 0 to Fe 3؉ via a respiratory chain that includes a bc 1 complex and a cytochrome c. Central carbon pathways were upregulated under aerobic conditions, correlating with higher growth rates, while many Calvin-Benson-Bassham cycle components were upregulated during anaerobic growth, probably as a result of more limited access to carbon dioxide. These results are important for understanding the role of A. ferrooxidans in environmental biogeochemical metal cycling and in industrial bioleaching operations.

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Section: Resultsmentioning

confidence: 99%

Anaerobic Sulfur Metabolism Coupled to Dissimilatory Iron Reduction in the Extremophile Acidithiobacillus ferrooxidans

Osório

Mangold

Denis

et al. 2013

Appl Environ Microbiol

137

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“…S' + 6Fe(III) + 4H20 -HSO4-+ 6Fe(II) + 7H+ HQNO (2-n-heptyl-4-hydroxyquinoline N-oxide) inhibited Fe(III) reduction with sulfur in T. ferrooxidans, and this fact has been provided as evidence that T. ferrooxidans should be able to conserve energy from this reaction (68). However, neither T. thiooxidans (169) nor T. ferrooxidans (277,303) was able to obtain energy to support growth from sulfur oxidation coupled to Fe(III) reduction.…”

Section: Sulfur-oxidizing Fe(iii) and Mn(iv) Reducersmentioning

confidence: 99%

Dissimilatory Fe(III) and Mn(IV) Reduction

Lovley

Holmes

Nevin

2004

Advances in Microbial Physiology

1,484

1,061

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“…So far, cytochromes c are components of all the S 0 -oxidation pathways described in the literature [30]. Furthermore, a bc 1 complex, and therefore a cytochrome c 1 , has been postulated to be involved in the S 0 -oxidizing respiratory system [4]. Because much more energy is available from S 0 than from FeII, a more likely explanation for the di¡erences observed in the total content of cytochromes c in S 0 -and FeII-grown cells is that the oxidation of FeII may require higher concentrations of electrontransport chain components, such as cytochromes c, to catalyze the rapid oxidation of FeII, as previously suggested by Ingledew [22].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the energy-dependent electron transfer pathway involved in the reduction of NAD(P) þ required for CO 2 ¢xation in FeII-grown cells has been proposed to include a bc1 complex [3]. A bc 1 complex has been also suggested to participate in the electron pathway from sulfur (S 0 ) to oxygen [4]. Therefore, c-type cytochromes seem to be involved in the oxidative pathways of both FeII and S 0 .…”

Section: Introductionmentioning

confidence: 99%

Cytochromes c of Acidithiobacillus ferrooxidans

Yarzábal¹

2002

FEMS Microbiology Letters

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The chemolithoautotrophic Gram-negative bacterium Acidithiobacillus ferrooxidans is versatile and can grow on a number of electron donors and acceptors. In the A. ferrooxidans ATCC 23270 genome, computer analysis identified 11 genes encoding putative cytochromes c. At least eight putative cytochromes c were differentiated on gels in ATCC 33020 cells grown on ferrous iron or sulfur. All these cytochromes were associated with the inner or the outer membranes. Lower levels of total cytochromes c were observed in sulfur-than in ferrous iron-grown cells. One cytochrome c was specific for sulfur conditions while three were specific for iron conditions, suggesting that cytochrome c synthesis is modulated depending on the electron donor. ß

show abstract

Is Fe3+/2+ cycling an intermediate in sulphur oxidation by Fe2+-grown Thiobacillus ferrooxidans

Cited by 18 publications

References 5 publications

Anaerobic Sulfur Metabolism Coupled to Dissimilatory Iron Reduction in the Extremophile Acidithiobacillus ferrooxidans

Anaerobic Sulfur Metabolism Coupled to Dissimilatory Iron Reduction in the Extremophile Acidithiobacillus ferrooxidans

Dissimilatory Fe(III) and Mn(IV) Reduction

Cytochromes c of Acidithiobacillus ferrooxidans

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