Is formalin fixation and ethanol preservation able to influence in geometric morphometric analysis? Fishes as a case study

Martínez, P.; Berbel‐Filho, Waldir M.; Jacobina, Uedson Pereira

doi:10.1007/s00435-012-0176-x

Cited by 57 publications

(57 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both species tended to contract, especially in the anterior dorsal area and the caudal peduncle, which has a tendency to fold. This pattern differs from those observed for Pomadasys corvinaerformis (Steindachner, 1868) (see Martinez et al 2013).…”

Section: Discussioncontrasting

confidence: 90%

“…Unfortunately, fresh fishes cannot always be collected, so the use of fishes found in collections or museums can sometimes be necessary. For this reason, the usefulness of creating an equation for determining the effect of preservation has been advocated (Simon 2013), but as Martinez et al (2013) emphasize, this is relatively simple when working with weight and length because they are straightforward measures. Using either 2D or 3D morphometry to predict changes produced by preservation in different body parts is much more complicated because knowledge of tissue composition and arrangement in each species is not readily available.…”

Section: Discussionmentioning

confidence: 99%

“…This approach permits analysis of morphological variation more comprehensively and provides a clearer view of deformation when thin plate splines are used (Bookstein 1989, Zelditch et al 2004. It also permits a direct comparison with the results obtained by Martinez et al (2013). Additionally, we carried out an exercise to explore potential deformation when making interspecific comparisons.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Effect of preservation procedures on the body shape of the golden mojarra, Diapterus aureolus (Actinopterygii: Perciformes: Gerreidae), and its repercussions in a taxonomic study

Vergara‐Solana¹,

Rodríguez²,

Cruz‐Agüero³

2014

Acta Ichthyol. Piscat

View full text Add to dashboard Cite

Section: Discussioncontrasting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Effect of preservation procedures on the body shape of the golden mojarra, Diapterus aureolus (Actinopterygii: Perciformes: Gerreidae), and its repercussions in a taxonomic study

Vergara‐Solana¹,

Rodríguez²,

Cruz‐Agüero³

2014

Acta Ichthyol. Piscat

View full text Add to dashboard Cite

“…Similarly, pressing and drying plant leaves can affect their shape noticeably [305] and may therefore have serious effects on studies of asymmetry. Many other types of specimens need to be stored in liquids such as ethanol or formalin, which changes tissue properties and also affects length measurements [306] and shape [307,308]. Even freezing can affect the shape of fish significantly [308].…”

Section: Preparation and Preservation Of Specimensmentioning

confidence: 99%

“…It may be possible to make measurements before and after preservation [305,[307][308][309][310], which makes it possible to quantify any artifacts and to their magnitude in relation to the biological effects under study. An even better option is to avoid preservation altogether.…”

Section: Preparation and Preservation Of Specimensmentioning

confidence: 99%

Analyzing Fluctuating Asymmetry with Geometric Morphometrics: Concepts, Methods, and Applications

2015

View full text Add to dashboard Cite

Approximately two decades after the first pioneering analyses, the study of shape asymmetry with the methods of geometric morphometrics has matured and is a burgeoning field. New technology for data collection and new methods and software for analysis are widely available and have led to numerous applications in plants and animals, including humans. This review summarizes the concepts and morphometric methods for studying asymmetry of shape and size. After a summary of mathematical and biological concepts of symmetry and asymmetry, a section follows that explains the methods of geometric morphometrics and how they can be used to analyze asymmetry of biological structures. Geometric morphometric analyses not only tell how much asymmetry there is, but also provide information about the patterns of covariation in the structure under study. Such patterns of covariation in fluctuating asymmetry can provide valuable insight about the developmental basis of morphological integration, and have become important tools for evolutionary developmental biology. The genetic basis of fluctuating asymmetry has been studied from empirical and theoretical viewpoints, but serious challenges remain in this area. There are many promising areas for further research that are only little explored at present.

show abstract

Phenotypic plasticity of a generalist fish species resident to lotic environments: Insights from the Great Lakes region

Hetzel,

Forsythe

2023

Ecology and Evolution

View full text Add to dashboard Cite

Fish morphology is incredibly plastic and local/resident morphology can be influenced by factors including habitat, predation, resource availability, and water velocity. Through analysis of body shape using geometric morphometrics, we describe the degree of phenotypic plasticity within a generalist fish species resident to low‐order tributaries of Green Bay and Lake Michigan. We predicted that isolated populations of creek chub (Semotilus atromaculatus) would display plastic responses in body shape due to differences in selective pressures imposed by stream environments. We show that body shape of creek chub was significantly different between streams which are considered to be isolated populations, and while we expected body shape variation to remain constant between study years, we found that shape was not fixed and changed over time in the same manner among focal streams. The diversity of creek chub diet and degree of agricultural land use in the watershed were significant predictors of body morphology. The effect of resource availability and land use within the watershed demonstrates how selective pressures influence phenotypes at the population level. Our study provides insight into morphological changes of stream fish populations, which may be important in the context of changing ecosystems and novel conditions.

show abstract

Is formalin fixation and ethanol preservation able to influence in geometric morphometric analysis? Fishes as a case study

Cited by 57 publications

References 27 publications

Effect of preservation procedures on the body shape of the golden mojarra, Diapterus aureolus (Actinopterygii: Perciformes: Gerreidae), and its repercussions in a taxonomic study

Effect of preservation procedures on the body shape of the golden mojarra, Diapterus aureolus (Actinopterygii: Perciformes: Gerreidae), and its repercussions in a taxonomic study

Analyzing Fluctuating Asymmetry with Geometric Morphometrics: Concepts, Methods, and Applications

Phenotypic plasticity of a generalist fish species resident to lotic environments: Insights from the Great Lakes region

Contact Info

Product

Resources

About