Is G1 arrest in plant seeds induced by a p53-related pathway?

Whittle, Carrie-Ann; Beardmore, Tannis; Johnston, Mark O.

doi:10.1016/s1360-1385(01)01952-5

Cited by 19 publications

(16 citation statements)

References 16 publications

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“…Also the UV-B-responsive ribosomal protein L10 was up-regulated, suggesting that it has a regulatory role in ribosome biogenesis (Ferreyra et al, 2010). Although no plant homologues of the p53 protein have so far been identified, the presence of a p53-like function sharing little sequence homology with the human counterpart cannot be ruled out (Whittle et al, 2001). In conclusion, all the reported data strongly suggest a key contribution of the MtTdp1α gene in the maintenance of nucleolar function.…”

Section: -Test) (B)mentioning

confidence: 83%

RNA-Seq analysis discloses early senescence and nucleolar dysfunction triggered by Tdp1α depletion in Medicago truncatula

Donà¹,

Confalonieri

Minio

et al. 2013

Journal of Experimental Botany

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An intron-spliced hairpin RNA approach was used for the targeted silencing of the MtTdp1α gene encoding the αisoform of tyrosyl-DNA phosphodiesterase 1 in Medicago truncatula Gaertn. Tyrosyl-DNA phosphodiesterase 1, involved in the repair of DNA topoisomerase I-mediated DNA damage, has been poorly investigated in plants. RNASeq analysis, carried out in the MtTdp1α-depleted plants, revealed different levels of transcriptional modulation (up-and down-regulation, alternative splicing, activation of alternative promoter) in genes involved in DNA damage sensing, DNA repair, and chromatin remodelling. It is suggested that the MtTdp1α gene has new, previously undetected roles in maintaining genome integrity. Up-regulation of senescence-associated genes and telomere shortening were observed. Moreover, impaired ribosome biogenesis indicated that the MtTdp1α gene is required for the nucleolar function. In agreement with the RNA-Seq data, transmission electron microscopy detected an altered nucleolar architecture in the MtTdp1α-depleted cells. Based on the reported data, a working hypothesis related to the occurrence of a nucleolar checkpoint in plant cells is proposed.

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Section: -Test) (B)mentioning

confidence: 83%

RNA-Seq analysis discloses early senescence and nucleolar dysfunction triggered by Tdp1α depletion in Medicago truncatula

Donà¹,

Confalonieri

Minio

et al. 2013

Journal of Experimental Botany

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“…Because the UV irradiation could cause damage to other cellular components, whether DNA damage could directly induce PCD in plant cells is still debatable. If a p53-related pathway exists in plants, as suggested by Whittle et al (2001), the p53-like gene might have little sequence homology with its mammalian counterpart.…”

Section: Possible Mechanisms Triggering Pcd In Tso2 Rnr2amentioning

confidence: 99%

ArabidopsisRibonucleotide Reductases Are Critical for Cell Cycle Progression, DNA Damage Repair, and Plant Development

2006

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Ribonucleotide reductase (RNR), comprising two large (R1) and two small (R2) subunits, catalyzes a rate-limiting step in the production of deoxyribonucleotides needed for DNA replication and repair. Previous studies in yeast and mammals indicated that defective RNR often led to cell cycle arrest, growth retardation, and p53-dependent apoptosis, whereas abnormally increased RNR activities led to higher mutation rates. Because plants are constantly exposed to environmental mutagens and plant cells are totipotent, an understanding of RNR function in plants is important. We isolated and characterized mutations in all three R2 genes (TSO2, RNR2A, and RNR2B) in Arabidopsis thaliana. tso2 mutants had reduced deoxyribonucleoside triphosphate (dNTP) levels and exhibited developmental defects, including callus-like floral organs and fasciated shoot apical meristems. tso2 single and tso2 rnr2a double mutants were more sensitive to UV-C light, and tso2 rnr2a seedlings exhibited increased DNA damage, massive programmed cell death, and release of transcriptional gene silencing. Analyses of single and double r2 mutants demonstrated that a normal dNTP pool and RNR function are critical for the plant response to mutagens and proper plant development. The correlation between DNA damage accumulation and the subsequent occurrence of apoptotic nuclei in tso2 rnr2a double mutants suggests that perhaps plants, like animals, can initiate programmed cell death upon sensing DNA damage.

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“…Since G1 CDK/cyclin complexes and the pRb/ E2F pathway, as well as the major DNA repair mechanisms, are also largely conserved in plants (Britt 1999;den Boer and Murray 2000a) it may be hypothesized that the G1/S checkpoint resembles those operating in animal cells and sensitive to DNA damage. In this context, it has been suggested that there is a remarkable parallel between the germination of aged plant seeds containing a high level of damaged DNA and the p53-mediated G1 arrest in mammals (Whittle et al 2001), since non-dormant older seeds show an increased mean germination time relative to their unaged counterparts. However, plant homologues of p53 and p21 have not been identified so far, although the entire genome of Arabidopsis has been sequenced (The Arabidopsis Genome Initiative 2000).…”

Section: Genomic Stability and Control Of The G1/s Transitionmentioning

confidence: 99%

“…Emerging evidence indicates that the plant G1/S transition, as in the case of other eukaryotes, is controlled by surveillance systems, termed checkpoints (den Boer and Murray 2000a;Whittle et al 2001). Furthermore, there are many examples indicating that the G1 phase is an important target for external signals that control plant growth.…”

Section: Introductionmentioning

confidence: 99%

Insights into the G1/S transition in plants

Rossi

Varotto

2002

Planta

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The G1/S transition generally represents the principal point of commitment to cell division. Many of the components of the cell cycle core machinery regulating the G1/S transition in plants have been recently identified. Although plant regulators of the G1/S transition display structural and biochemical homologies with their animal counterparts, their functions in integrating environmental stimuli and the developmental program within cell cycle progression are often plant-specific. In this review, recent progress in understanding the role of plant G1/S transition regulators is presented. Emerging evidence concerning the mechanisms of G1/S control in response to factors triggering the cell cycle and the integration of these mechanisms with plant development is also discussed.

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Is G1 arrest in plant seeds induced by a p53-related pathway?

Cited by 19 publications

References 16 publications

RNA-Seq analysis discloses early senescence and nucleolar dysfunction triggered by Tdp1α depletion in Medicago truncatula

RNA-Seq analysis discloses early senescence and nucleolar dysfunction triggered by Tdp1α depletion in Medicago truncatula

ArabidopsisRibonucleotide Reductases Are Critical for Cell Cycle Progression, DNA Damage Repair, and Plant Development

Insights into the G1/S transition in plants

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