Is HPV-18 present in human breast cancer cell lines?

Abstract

Sir,Although several studies have suggested an association between breast cancer and human papillomavirus (HPV) infection (de Villiers et al, 2005;Cazzaniga et al, 2009), two recent papers published in the British Journal of Cancer Lawson et al, 2009) were particularly provocative. The authors claimed that both primary human breast cancers and two well-characterised breast cancer cell lines (MDA-MB-175VII and SK-Br-3) contained HPV-18, a type of HPV found with increased frequency in adenocarcinomas (Iwasawa et al, 1996;Burk et al, 2003).To further characterise the MDA-MB-175VII and SK-Br-3 cell lines for HPV-18 gene content and expression, we performed PCR and RT -PCR to detect viral DNA and mRNA. We also included two cell lines as controls. The HeLa cervical cancer cell line contains HPV-18 and was used as a positive control and the C33 cervical cancer cell line contains no detectable HPV genomes. All cell lines were grown in 10% FBS DMEM media to approximately 85% confluency and then harvested for DNA and RNA isolation, after which we performed standard PCR and RT -PCR reactions using the indicated primer sets for the early, late, and non-coding regions of HPV-18 (see Figure 1A). For reference, we also used the exact L1

Epstein-Barr Virus, Human Papillomavirus and Mouse Mammary Tumour Virus as Multiple Viruses in Breast Cancer

“…Each of these viruses has known oncogenic potential and has been identified in normal and malignant human breast tissues. However, the identification of HPV, MMTV and EBV in breast tumours has been challenged [3] – [5] . The reasons for these challenges vary and include failure of some investigators to identify viral sequences in breast tumours and breast cancer cultured cell lines, concern about contamination of analyses based on polymerase chain reaction (PCR) techniques, and concern that outcomes based on immunohistochemistry may be non-specific and due to cross reacting proteins.…”

The Effect of Indian Fig Fruit Extract on Human Papilloma Virus containing Cervical Cancer Cells (HeLa) by Decreasing the HPV18 L1 Gene Load

“…Analysing the presence of Human Papilloma Virus (HPV), its types and its viral load is the basic step in evaluating the risk of cervical cancer and most of the available data indicate that the cervical lesions are related to HPV-16 and HPV-18 (Clifford et al, 2006). The HeLa cell line was used as positive control for the presence of HPV 18 in many studies (Peran et al, 2010;Xiao et al, 2015). The latex of fig fruit and other parts were reported to be traditionally used for HPV treatment.…”

O Possível Envolvimento do HPV no Câncer de Mama

“…Relatam que o tipo mais prevalente em ambos foi o HPV-11, seguido pelo HPV-6. Outros tipos detectados foram os HPV 16, 23, 27 e 57 (mamilos e carcinomas), HPV 20,21,32,37,38, 66 e GA3-1 (mamilos apenas) e HPV 3, 15, 24, 87, DL473 (carcinomas apenas), e vários tipos foram demonstrados em sete carcinomas e dez amostras de mamilo.…”