IS
            <i>26</i>
            Veers Genomic Plasticity and Genetic Rearrangement toward Carbapenem Hyperresistance under Sublethal Antibiotics

Wei, Dawei; Wong, Nai-Kei; Song, Yuqin; Zhang, Gang; Wang, Chao; Li, Juan; Feng, Jie

doi:10.1128/mbio.03340-21

Cited by 12 publications

(13 citation statements)

References 27 publications

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“…IS 26 was regarded as a vital mobile element contributed to the dissemination and rearrangement of resistance determinants, as well as plasmid reorganization ( Harmer and Hall, 2015 ; He et al, 2015 ; Wei et al, 2022 ). A previous study demonstrated that IS 26 was involved in recombination of an IncN1-F33: A-: B- plasmid and an mcr-1 -carrying phage-like plasmid during conjugation process, facilitating the dissemination of mcr-1 ( He et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

“…Subsequently, the genetic rearrangement of a 9,379 bp fragment organized as IS6100-orf3-IS15DI-repB(N)-IS5075-IS26-mph(A) immediately upstream of the tnpA gene and downstream of the tnpR gene, causing the insertion of macrolide resistance determinant containing part IS15DI-repB(N)-IS5075-IS26-mph(A) in the backbone region and the integration of the remnant part of the 2,512 bp fragment between the tnpA gene and the IS15DI, resulting in the formation of Module B. Finally, insertion of the IS26-tet(M)-orf4-tnp-IS5075 element, conferring resistance to tetracyclines, as previously described in pHS13-1-IncHI2 6 https://tool-mml.sjtu.edu.cn/oriTfinder/oriTfinder.html IS26 was regarded as a vital mobile element contributed to the dissemination and rearrangement of resistance determinants, as well as plasmid reorganization (Harmer and Hall, 2015;He et al, 2015;Wei et al, 2022). A previous study demonstrated that IS26 was involved in recombination of an IncN1-F33: A-: Bplasmid and an mcr-1-carrying phage-like plasmid during conjugation process, facilitating the dissemination of mcr-1 (He et al, 2019).…”

Section: Proposed Formation Mechanism Of the Plasmid Pec15-mcr-50mentioning

confidence: 99%

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Emergence of a novel hybrid mcr-1-bearing plasmid in an NDM-7-producing ST167 Escherichia coli strain of clinical origin

et al. 2022

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Colistin is considered as an antibiotic of ‘last resort’ for the treatment of lethal infections caused by carbapenem-resistant Enterobacterales (CRE), dissemination of plasmid-borne colistin resistance gene mcr-1, particularly into CRE, resulting in the emergence of strains that approach pan-resistance. A wide variety of plasmid types have been reported for carrying mcr-1. Among which, large IncHI2-type plasmids were multidrug-resistant (MDR) plasmids harbored multiple resistance determinants in addition to mcr-1. Herein, we characterized a novel hybrid IncHI2-like mcr-1-bearing plasmid in an NDM-7-producing ST167 Escherichia coli strain EC15-50 of clinical origin. Antimicrobial susceptibility testing showed E. coli EC15-50 exhibited an extensively drug-resistant (XDR) profile that only susceptible to amikacin and tigecycline. S1-PFGE, Southern hybridization and Whole-genome Sequencing (WGS) analysis identified a 46,161 bp blaNDM-7-harboring IncX3 plasmid pEC50-NDM7 and a 350,179 bp mcr-1-bearing IncHI2/HI2A/N/FII/FIA plasmid pEC15-MCR-50 in E. coli EC15-50. Sequence detail analysis revealed the type IV coupling protein (T4CP) gene was absent on pEC15-MCR-50, explaining that pEC15-MCR-50 was a non-conjugative plasmid. Comparative genetic analysis indicated the hybrid plasmid pEC15-MCR-50 was probably originated from pXGE1mcr-like IncHI2/HI2A/N plasmid and pSJ_94-like IncFII/FIA plasmid, and generated as a result of a replicative transposition process mediated by IS26. Currently, the prevalent mcr-1-carrying IncHI2 plasmids were rarely reported to be fused with other plasmids. The identification of the novel hybrid plasmid pEC15-MCR-50 in this study highlighted the importance of close surveillance for the emergence and dissemination of such fusion MDR plasmids, particularly in NDM-producing Enterobacterales.

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Section: Resultsmentioning

confidence: 99%

Section: Proposed Formation Mechanism Of the Plasmid Pec15-mcr-50mentioning

confidence: 99%

Emergence of a novel hybrid mcr-1-bearing plasmid in an NDM-7-producing ST167 Escherichia coli strain of clinical origin

et al. 2022

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“…First of all, the sequencing bias introduced by library preparation can lead to the gene copy number being over- or underestimated ( 27 ). Second, as CCNE determines the average gene copy number of a clone community or population, it will produce an estimate of less than one gene copy if the bacterial cells in the community lost the gene, especially when the gene is located on a transposon or plasmid ( 28 ).…”

Section: Discussionmentioning

confidence: 99%

Carbapenemase-Encoding Gene Copy Number Estimator (CCNE): a Tool for Carbapenemase Gene Copy Number Estimation

Jiang

Chen

et al. 2022

Microbiol Spectr

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“…39,66 Vaccinia immune globulin intravenous (VIGIV) has also been found useful in treating severe cases of MPX as well as prospective passive antibody-based therapies such as hyperimmune globulin (HIG), monoclonal antibodies (mAbs), and convalescent plasma (CP) have also been suggested to play useful role for treating MPX, particularly beneficial against severe cases. 72 The replicative cycle of MPOX virus in humans and the therapeutic interventions by the anti-MPX viral drugs have been elucidated in Figure . Tecovirimat, which is an orthopoxvirusspecific protein (VP37) inhibitor harbors the…”

Section: Anti-viral Drugs and Therapeutic Interventionsmentioning

confidence: 99%

An overview on Monkeypox, Current Paradigms and Advances in its Vaccination, Treatment and Clinical Management: Trends, Scope, Promise and Challenges

Sharma¹,

Aggarwal²,

Sharma³

et al. 2022

J. Pure Appl. Microbiol.

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Monkeypox virus is an orthopoxvirus sharing the common genus with variola and vaccinia virus. Most of the monkeypox (MPX) cases had been reported from the central and west African region (the main endemic areas) prior to 2022 but there was a sudden outbreak in May, 2022 disseminating the infections to thousands of people even in non-endemic countries, posing a global public health emergency. MPX was considered a rae and neglected disease, however the 2022 MPX outbreaks in multiple countries attracted attention of worldwide researchers to pace up for carrying out researches on various aspects of MPXV including attempts to design and develop diagnostics, vaccines, drugs and therapeutics counteract MPX. Apart from being a zoonotic disease, the current outbreaks highlighted rapid human-to-human transmission of MPXV, besides the reverse zoonosis has also been documented with recent first report of human-to-dog transmission, urging a call for the importance of one health approach. Atypical and unusual disease manifestations as well asymptomatic MPXV infections have also been observed during 2022 MPX outbreak. The affected patients typically develop a rash resulting in a mild disease followed by recovery with some supportive care and use of antivirals such as tecovirimat, cidofovir and brincidofovir in severe disease cases. Modified vaccinia Ankara (MVA) vaccine with an excellent safety profile has been recommended to patients with higher risk exposure and immunocompromised individuals. Moreover, another vaccine the replication-competent vaccine (ACAM2000) could be a suitable alternative to MVA’s non-availability to some selective immunocompetent individuals. Current review highlights the salient aspects of management and treatment of monkeypox along with underlying promises in terms of therapeutics and a variety of challenges posed due to current global public health emergency situation to counteract MPX.

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IS 26 Veers Genomic Plasticity and Genetic Rearrangement toward Carbapenem Hyperresistance under Sublethal Antibiotics

Cited by 12 publications

References 27 publications

Emergence of a novel hybrid mcr-1-bearing plasmid in an NDM-7-producing ST167 Escherichia coli strain of clinical origin

Emergence of a novel hybrid mcr-1-bearing plasmid in an NDM-7-producing ST167 Escherichia coli strain of clinical origin

Carbapenemase-Encoding Gene Copy Number Estimator (CCNE): a Tool for Carbapenemase Gene Copy Number Estimation

An overview on Monkeypox, Current Paradigms and Advances in its Vaccination, Treatment and Clinical Management: Trends, Scope, Promise and Challenges

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