Is IS<sub>ABA-1</sub>customized for<i>Acinetobacter</i>?

Segal, Heidi; Garny, Seike; Elisha, B. Gay

doi:10.1016/j.femsle.2005.01.005

Cited by 141 publications

(102 citation statements)

References 15 publications

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“…In the case of ISAba1, this element has provided the entire promoter sequences for bla OXA-58 expression, as demonstrated in different A. baumannii isolates in which ISAba1 enhanced expression of the bla AmpC cephalosporinase gene or the aac(3)-IIa acetyltransferase gene (7,13,26). The exact same motifs belonging to ISAba1 were acting as promoter sequences in these latter cases, thus confirming that ISAba1 is a useful tool for gene expression in A. baumannii and might be customized for that species, as suggested by Segal et al (25). The frequent association between bla OXA-58 gene and IS elements could be the consequence of low-level expression of this oxacillinase gene in a wild-type position.…”

Section: Discussionsupporting

confidence: 66%

Acinetobacter baumannii

2015

Reviews in Medical Microbiology

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Genetic structures surrounding the carbapenem-hydrolyzing oxacillinase gene bla OXA-58 were characterized in a series of OXA-58-positive Acinetobacter baumannii strains isolated from different countries. We showed that in most of the cases, acquisitions of the bla OXA-58 -containing overall structure, including insertion sequence elements, may be likely the results of recombination events. In type strain A. baumannii MAD, the genetic structure surrounding the bla OXA-58 gene was bracketed by two 27-bp repeated sequences. The isolation of a clonally related OXA-58-negative A. baumannii isolate that possessed the same plasmid backbone as A. baumannii MAD but lacked this genetic structure indicated that the mechanism of acquisition could be reversible. Parts of the structure identified in A. baumannii MAD were conserved in other bla OXA-58 -positive isolates from various European countries. Primer extension experiments showed that bla OXA-58 expression was related to promoter sequences brought by different insertion sequence elements, such as an ISAba3-like element, ISAba1, ISAba2, and IS18. This work identified novel structures at the origin of acquisition and expression of a carbapenem-hydrolyzing ␤-lactamase identified in non-clonally related A. baumannii isolates.

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Section: Discussionsupporting

confidence: 66%

Acinetobacter baumannii

2015

Reviews in Medical Microbiology

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“…3) (GenBank accession number GQ421466). A part of the region of the DNA sequence was similar to a previously reported DNA sequence of A. baumannii (14), indicating a horizontal gene transfer. Using the BPROM program, a putative promoter composed of Ϫ35 (TTGAGA) and Ϫ10 (CGTTAGAAA) elements was located upstream of sulII and within ISAba1.…”

supporting

confidence: 80%

Bacteremia Due to Acinetobacter Genomic Species 10

et al. 2010

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“…The presence of the ISAba1 promoter sequence in the isolates and its association with the bla OXA-23-like and bla OXA-51-like genes were investigated by PCR (12) (26) (27) . The primer sequences and the sizes of the expected reaction products are outlined in Table 1.…”

Section: Investigation Of the Genetic Elements Associated With Oxacilmentioning

confidence: 99%

The role of the genetic elements bla oxa and IS Aba 1 in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex in carbapenem resistance in the hospital setting

Kobs¹,

Ferreira

Bobrowicz

et al. 2016

Rev. Soc. Bras. Med. Trop.

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Introduction: Members of the Acinetobacter genus are key pathogens that cause healthcare-associated infections, and they tend to spread and develop new antibiotic resistance mechanisms. Oxacillinases are primarily responsible for resistance to carbapenem antibiotics. Higher rates of carbapenem hydrolysis might be ascribed to insertion sequences, such as the ISAba1 sequence, near bla OXA genes. The present study examined the occurrence of the genetic elements bla OXA and ISAba1 and their relationship with susceptibility to carbapenems in clinical isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. Methods: Isolates identifi ed over 6 consecutive years in a general hospital in Joinville, Southern Brazil, were evaluated. The investigation of 5 families of genes encoding oxacillinases and the ISAba1 sequence location relative to bla OXA genes was conducted using polymerase chain reaction. Results: All isolates presented the bla OXA-51-like gene (n = 78), and 91% tested positive for the bla OXA-23-like gene (n = 71). The presence of ISAba1 was exclusively detected in isolates carrying the bla OXA-23-like gene. All isolates in which ISAba1 was found upstream of the bla OXA-23-like gene (n = 69) showed resistance to carbapenems, whereas the only isolate in which ISAba1 was not located near the bla OXA-23-like gene was susceptible to carbapenems. The ISAba1 sequence position of another bla OXA-23-like -positive isolate was inconclusive. The isolates exclusively carrying the bla OXA-51-like gene (n = 7) showed susceptibility to carbapenems. Conclusions: The presence of the ISAba1 sequence upstream of the bla OXA-23-like gene was strongly associated with carbapenem resistance in isolates of the A. calcoaceticus-A. baumannii complex in the hospital center studied.

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Is IS_ABA-1customized forAcinetobacter?

Cited by 141 publications

References 15 publications

Acinetobacter baumannii

Acinetobacter baumannii

Bacteremia Due to Acinetobacter Genomic Species 10

The role of the genetic elements bla oxa and IS Aba 1 in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex in carbapenem resistance in the hospital setting

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Is ISABA-1customized forAcinetobacter?

Cited by 141 publications

References 15 publications

Acinetobacter baumannii

Acinetobacter baumannii

Bacteremia Due to Acinetobacter Genomic Species 10

The role of the genetic elements bla oxa and IS Aba 1 in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex in carbapenem resistance in the hospital setting

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Is IS_ABA-1customized forAcinetobacter?