“…It is the ultimate output of plant N absorption and assimilation efficiency of absorbed N. Agronomic operations, the use of controlled released or enhanced efficiency fertilizers, incorporation or impregnation of urea fertilizer to various organic or inorganic materials can significantly increase NUE and yield of corn (Latifah et al, 2017;Martins et al, 2017;Zhang et al, 2017;Kubota et al, 2018). The conventional tillage in the rain-fed condition also increased corn yield and NUE (Pareja-Sánchez et al, 2019) while urea top dressing decreased corn grain yield by 6.8%-9.8%, NUE by 13.2%-14.3% with annual net loss increased by 15.4%-21.8% compared to coated urea at the similar rates of N application (Zheng et al, 2017). In addition, the N uptake in grain increases with the increase of N application frequency compared to a single or double application, while total N uptake and biomass production also increase significantly (Lü et al, 2012).…”