In the contemporary era, as a result of the radical progress of technology through digitization, business ecosystems are transforming into structures that are more competitive, innovative, and capable of responding rapidly to change. Throughout this transformative process, the concept of leadership has evolved towards more diverse perspectives, building on its significance since the Industrial Revolution. Beyond the traditional notion of leadership, the concept of self-leadership emerges as a crucial tool in the rapidly changing dynamics of competitive business environments. Research suggests that the characteristics of self-leadership vary according to individuals' personality structures. Due to the insufficient exploration of studies concerning the relationship between individuals' self-leadership and their personality structures within Turkish culture, this study was conducted. In this perspective, a quantitative research methodology was employed by researchers, with a focus on relational analysis among variables. The study involved the administration of self-leadership and the Big Five personality traits scales to 349 participants. Gender and sectoral differences in the relationship between self-leadership and the five personality traits were also examined. The analyses revealed significant effects of the five personality traits on self-leadership in the overall context (β: .376, p < .05). The extraversion personality trait exhibited a significant impact on self-leadership (β: .322, p < .05). Openness to experience had a significant effect on self-leadership (β: .344, p < .05), as did the responsibility personality trait (β: .348, p < .05). When examined from a gender perspective, the gender factor was identified as a significant variable in the impact of the agreeableness personality trait on self-leadership (β1: .247; β2: .192, p < .05). Additionally, the gender factor was found to be a significant variable in the impact of extraversion personality trait on self-leadership (β1: .271; β2: .382, p < .05). Furthermore, when exploring differences between individuals working in the public or private sectors, the sector factor was identified as a significant variable in the impact of emotional stability personality trait on self-leadership (β1: .243; β2: .337, p < .05).