Is it possible to study the kinetic parameters of interaction between PNA and parallel and antiparallel DNA by stopped-flow fluorescence?

Barbero, Nadia; Cauteruccio, Silvia; Thakare, P.; Licandro, Emanuela; Viscardi, G.; Visentin, Sonja

doi:10.1016/j.jphotobiol.2016.08.007

Cited by 1 publication

(2 citation statements)

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“…The PNA was synthesized as previously described. [ 90 ] Before encapsulation, HSA and PNA were bioconjugated with FITC following the reported protocol. [ 96 ] The excess of FITC was 50‐fold the moles of HSA or PNA.…”

Section: Methodsmentioning

confidence: 99%

“…[85,86] Given the high binding capability of mucins, we investigated if this property could be exploited to encapsulate macromolecules within mucosomes. [27,[87][88][89] A small peptide nucleic acid (PNA) (TCACTAGATG, MW < 10 kDa), [90] and human serum albumin (HSA; MW < 100 kDa) were selected as representative models of nucleic acids and proteins, respectively. Despite their high molecular weight, the two macromolecules showed excellent encapsulation efficiencies.…”

Section: Mucosomes Are a Versatile Drug Carriermentioning

confidence: 99%

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Mucosomes: Intrinsically Mucoadhesive Glycosylated Mucin Nanoparticles as Multi‐Drug Delivery Platform

Butnarasu

Petrini

Bracotti

et al. 2022

Adv Healthcare Materials

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Mucus is a complex barrier for pharmacological treatments and overcoming it is one of the major challenges faced during transmucosal drug delivery. To tackle this issue, a novel class of glycosylated nanoparticles, named "mucosomes," which are based on the most important protein constituting mucus, the mucin, is introduced. Mucosomes are designed to improve drug absorption and residence time on the mucosal tissues. Mucosomes are produced (150-300 nm), functionalized with glycans, and loaded with the desired drug in a single one-pot synthetic process and, with this method, a wide range of small and macro molecules can be loaded with different physicochemical properties. Various in vitro models are used to test the mucoadhesive properties of mucosomes. The presence of functional glycans is indicated by the interaction with lectins. Mucosomes are proven to be storable at 4 °C after lyophilization, and administration through a nasal spray does not modify the morphology of the mucosomes. In vitro and in vivo tests indicate mucosomes do not induce adverse effects under the investigated conditions. This study proposes mucosomes as a ground-breaking nanosystem that can be applied in several pathological contexts, especially in mucus-related disorders.

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Section: Methodsmentioning

confidence: 99%