Is it possible to substitute hexane with green solvents for extraction of carotenoids? A theoretical versus experimental solubility study

Yara-Varón, Edinson; Fabiano-Tixier, Anne Sylvie; Balcells, Mercè; Canela‐Garayoa, Ramon; Bily, Antoine; Chémat, Farid

doi:10.1039/c6ra03016e

Cited by 152 publications

(92 citation statements)

References 18 publications

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“…Measurement of the carotenoid content and the NCED, ZEP, and AO activities The carotenoid content was measured with a UV-2450 spectrophotometer (Shimadzu). 30 Peach mesocarps (5 g) were homogenized in 8 mL acetone and incubated in darkness at 4°C for 24 h. After centrifugation at 10 000×g for 5 min at 4°C, the supernatant was collected and the absorbance at 450 nm was recorded. The carotenoid content was expressed as milligrams per kilogram.…”

Section: Measurement Of Spermidine and Spermine Contentsmentioning

confidence: 99%

Regulation of the biosynthesis of endogenous nitric oxide and abscisic acid in stored peaches by exogenous nitric oxide and abscisic acid

et al. 2020

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BACKGROUND Nitric oxide (NO) and abscisic acid (ABA) are important regulators of plant response to cold stress, and they interact in response to cold signals. The primary goal of this study was to determine the roles of exogenous NO and ABA on the synthesis of endogenous NO and ABA in cold‐stored peach fruit. RESULTS Exogenous NO and ABA maintained a relatively high content of NO, increased nitrate reductase (NR) activity, and inhibited the activity of NO synthase (NOS)‐like and the levels of polyamine biosynthesis in peaches during cold storage. Treatments of potassium 2‐(4‐carboxyphenyl)‐4,4,5,5‐tetramethylimidazoline‐1‐oxyl‐3‐oxide (c‐PTIO), NO, N‐nitro‐l‐Arg‐methyl ester (L‐NAME), and sodium tungstate did not influence ABA content. Exogenous ABA increased the content of carotenoids and the activities of aldehyde oxidase (AO), 9‐cis‐epoxycarotenoid dioxygenase (NCED), and zeaxanthin epoxidase (ZEP) of ABA synthesis in peaches during cold storage, and upregulated the gene expression of PpAO1, PpNCED1, PpNCED2, and PpZEP. The production of endogenous NO was differentially inhibited by NO scavengers, ABA inhibitors, and NR inhibitors, but not affected by NOS‐like inhibitors during cold storage. CONCLUSION Exogenous NO and ABA can induce endogenous NO synthesis in cold‐stored peaches by the nitrate reductase pathway, and ABA can mediate endogenous ABA synthesis by the autocatalytic reaction. NO does not regulate ABA synthesis. © 2019 Society of Chemical Industry

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Section: Measurement Of Spermidine and Spermine Contentsmentioning

confidence: 99%

Regulation of the biosynthesis of endogenous nitric oxide and abscisic acid in stored peaches by exogenous nitric oxide and abscisic acid

et al. 2020

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“…[63] appliedt he previously-mentioned dual approach (theoretical investigation by using both HSPs and COSMO-RS, ande xperimental quantification by HPLC and UV/Vis spectroscopy) to evaluate the capability of five eco-friendly solvents (CPME,2 -MeTHF,I PA,E tOAc and DMC) as hexane-substitutes for the extraction of carotenoids from carrots. [63] appliedt he previously-mentioned dual approach (theoretical investigation by using both HSPs and COSMO-RS, ande xperimental quantification by HPLC and UV/Vis spectroscopy) to evaluate the capability of five eco-friendly solvents (CPME,2 -MeTHF,I PA,E tOAc and DMC) as hexane-substitutes for the extraction of carotenoids from carrots.…”

Section: Introduction:m Otivation For Eco-friendly Solvents and For Cpmementioning

confidence: 99%

“…With respect to extractions, Yada-Varóne tal. [63] appliedt he previously-mentioned dual approach (theoretical investigation by using both HSPs and COSMO-RS, ande xperimental quantification by HPLC and UV/Vis spectroscopy) to evaluate the capability of five eco-friendly solvents (CPME,2 -MeTHF,I PA,E tOAc and DMC) as hexane-substitutes for the extraction of carotenoids from carrots. Based on the HSPs analysis, nonpolaro r slightly polar solvents were the most suitable solvents for extraction of carotenoids,w hereas COSMO-RS analysiss howeda higher probability of solubility for all carotenoids from carrot in CPME,2 -MeTHF,a nd ethyl acetate than in hexane.…”

Section: Introduction:m Otivation For Eco-friendly Solvents and For Cpmementioning

confidence: 99%

Cyclopentyl Methyl Ether (CPME): A Versatile Eco‐Friendly Solvent for Applications in Biotechnology and Biorefineries

Gonzalo

Alcántara

Marı́a³

2019

ChemSusChem

121

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The quest for sustainable solvents is currently a matter of intense research and development, as solvents significantly contribute heavily to the waste generated by chemical industries. Cyclopentyl methyl ether (CPME) is a promising eco‐friendly solvent with valuable properties such as low peroxide formation rate, stability under basic and acidic conditions, and relatively high boiling point. This Review discusses the potential use of CPME for applications in biotechnology (e.g., biotransformations, as solvent or cosolvent), biorefineries, and bioeconomy (e.g., for furan synthesis or as an extractive agent in liquid–liquid separations), as well as for other purposes, such as chromatography or peptide synthesis. Although CPME is currently produced by petrochemical means with a remarkably high atom economy, its biogenic production can be envisaged from substrates such as cyclopentanol or cyclopentanone, which can be derived from furfural or from (bio‐based) adipic acid, respectively. The combination of the promising properties of CPME as a (co)solvent with a future (economic) biogenic origin would be advantageous for setting strategies aligned with the sustainable chemistry principles.

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“…The CPME extract contained greater concentrations of chlorophyll a (2.85 ± 0.01 mg/g dry cells ) and carotenoids (1.63 ± 0.01 mg/g dry cells ) than hexane, whereas chlorophyll b appeared to be significantly less extractable in both of them than EA. In the work of Yara‐Varón et al ., CPME was used as the best extracting solvent for carotenoids. No other references in the literature were found indicating that these two green solvents were used for chlorophyll extraction.…”

Section: Resultsmentioning

confidence: 99%

Membrane‐assisted biorefinery of microalgae to obtain enriched fractions of bioderived molecules

Djamai

Mazzei

Bazzarelli

et al. 2019

Biofuels Bioprod Bioref

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Microalgae represent an important source of valuable bioproducts (such as food, nutraceuticals, antioxidants, and pigments) and biofuel. They grow spontaneously in surface water and represent a contaminant that has to be separated. There is thus an urgent need to develop technologies to harvest and refine them. Depending on the microalgae species, different biocompounds can be found and appropriate separation and purification methods must be refined and selected. In this work, microalgae harvested in the Hammam Boughrara dam, situated in Tlemcen (north-west of Algeria), were used as source to recover and fractionate bio-derived molecules using membrane-assisted processes. This study identified a suitable strategy to fractionate biomolecules using ultrafiltration (UF) membranes. To maintain a stable permeate flux it was necessary to decrease the complexity of the solution prior to UF. This was achieved by separating triacylglycerols (TAGs) and pigments, after breaking cells, by solid-liquid-liquid extraction with a green solvent. The UF of the remaining aqueous phase permitted a recovery factor of 70% of the proteins and more than 85% of the carbohydrates in the retentate. The TAGs that were not recovered in the green solvent, and remained in the aqueous phase, were separated in the permeate with a purity of about 70% and a recovery factor of about 60%.

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Is it possible to substitute hexane with green solvents for extraction of carotenoids? A theoretical versus experimental solubility study

Abstract: Evaluation of five green solventsi.e.2-methyltetrahydrofuran (2-MeTHF), dimethyl carbonate (DMC), cyclopentyl methyl ether (CPME), isopropyl alcohol (IPA) and ethyl acetate, for replacing of hexane in extraction of carotenoids from carrots.

Cited by 152 publications

References 18 publications

Regulation of the biosynthesis of endogenous nitric oxide and abscisic acid in stored peaches by exogenous nitric oxide and abscisic acid

Regulation of the biosynthesis of endogenous nitric oxide and abscisic acid in stored peaches by exogenous nitric oxide and abscisic acid

Cyclopentyl Methyl Ether (CPME): A Versatile Eco‐Friendly Solvent for Applications in Biotechnology and Biorefineries

Membrane‐assisted biorefinery of microalgae to obtain enriched fractions of bioderived molecules

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