“…These innovations typically target one or more of the driving factors influencing beef tenderness including postmortem proteolysis, background toughness, and sarcomere length ( Warner et al, 2022 ). In general, cull cow beef would be categorized as having a lower extent of postmortem proteolysis ( Cruzen et al, 2014 ; Xiong et al, 2007 ), a high concentration of highly cross-linked, insoluble collagen ( Alvarenga et al, 2021 ; Dos Santos Fontes et al, 2021 ; Jurie et al, 2007 ), and potentially shorter sarcomeres if rapid chilling occurs ( Gredell et al, 2018 ; Lucero-Borja et al, 2014 ). Post-harvest processing techniques during carcass chilling (e.g., electrical stimulation, delay chilling, hot boning, skeletal separation, vacuum impregnation; Table 1 ), postmortem aging (e.g., wet aging, dry aging; Table 2 ), physical interventions (e.g., blade/needle tenderization, tumbling, hydrodynamic shockwaves, ultrasonication, high-pressure processing, pulsed electric field; Table 3 ), and enhancement with functional ingredients have great promise in improving the tenderness and palatability traits of beef from mature carcasses ( Table 4 ).…”