Is NMR-based metabolomic analysis of exhaled breath condensate accurate?

Izquierdo-García, José Luis; Peces-Barba, Germán; Heili, Sarah; Diaz, R; Want, Elizabeth J.; Ruíz-Cabello, Jesús

doi:10.1183/09031936.00094010

Cited by 22 publications

(18 citation statements)

References 18 publications

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“…However, as no NMR details were found (i.e. the operating magnetic field, the number of acquisitions, the possible use of a cryoprobe, and the detection limit), it is difficult to conclude that NMR spectroscopy cannot be used to analyse EBC samples because it does not have the sensitivity required to observe the endogenous metabolites in the EBC [7]. With our spectrometer set-up, we have estimated a detection limit that is notably low for NMR-based metabolomics [10].…”

Section: To the Editorsmentioning

confidence: 99%

“…By using an Anacon condenser (Biostec, Valencia, Spain), different results have been reported [7]. Collection devices are an important source of variability of EBC biomarkers [2,3,8].…”

Section: To the Editorsmentioning

confidence: 99%

“…This approach has recently been questioned as NMR-based metabolomics of EBC collected using a condenser with reusable parts was reported to be affected by cleaning procedures, generating artificial signals that were not related to the endogenous metabolites of the lungs [7].…”

Section: To the Editorsmentioning

confidence: 99%

“…In the score plot, t [1], t [2] and t [3] c EUROPEAN RESPIRATORY JOURNAL VOLUME 39 NUMBER 2 reusable-part condenser [4], and results obtained by others using a non-reusable-part condenser and mass spectrometry (MS) [7].…”

Section: To the Editorsmentioning

confidence: 99%

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Nuclear magnetic resonance-based metabolomics of exhaled breath condensate: methodological aspects

Motta¹,

Paris²,

Melck³

et al. 2012

Eur Respir J

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Section: To the Editorsmentioning

confidence: 99%

“…By using an Anacon condenser (Biostec, Valencia, Spain), different results have been reported [7]. Collection devices are an important source of variability of EBC biomarkers [2,3,8].…”

Section: To the Editorsmentioning

confidence: 99%

Section: To the Editorsmentioning

confidence: 99%

Section: To the Editorsmentioning

confidence: 99%

See 2 more Smart Citations

Nuclear magnetic resonance-based metabolomics of exhaled breath condensate: methodological aspects

Motta¹,

Paris²,

Melck³

et al. 2012

Eur Respir J

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“…NMR-based metabolomics of EBC may have a potential use in characterising respiratory diseases as it was able to discriminate patients with COPD [ 104 ], asthma [ 106 ], stable and unstable cystic fibrosis [ 107 ] from healthy subjects. In addition, a recent NMR spectroscopic study has also shown that the repeated use of the EcoScreen ® condenser does not cause any carry-over effect and the disinfectant Milton does not alter EBC metabolomics [ 100 ], as opposed to another study that reported artificial signals when the Anacon condenser was used and disinfected with Descogen [ 108 ]. Different analytical techniques, including NMR and mass spectro metry, may be combined to improve sensitivity and further consolidate ' breathomics ' [ 100 ].…”

Section: Nmr Spectroscopy-based Metabolomicsmentioning

confidence: 99%

Exhaled breath condensate: a comprehensive update

Ahmadzai

Huang²,

Hettiarachchi³

et al. 2013

Clinical Chemistry and Laboratory Medicine

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Since the late 1990s, a surge in interest in the analysis of exhaled breath condensate (EBC) resulted in the American Thoracic Society and European Respiratory Society (ATS/ERS) organising a Task Force in 2001 to develop guidelines on EBC collection and measurement of biomarkers. This Task Force published their guidelines in 2005 based on literature and expert opinions at that time, and multiple shortcomings and knowledge deficits were also identified. The clinical application of EBC collection and its biomarkers are currently still limited by several of these knowledge gaps, hence further guidelines for standardisation are required to ensure external validity. Using related articles produced since the publication of the ATS/ERS Task Force report, this paper attempts to provide a comprehensive update to the original guideline and review the methodological shortcomings identified. This review can hopefully serve as a yardstick for future studies involving this emerging clinical tool.

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Biomarkers for respiratory diseases: Present applications and future discoveries

Liu

Cui

Wang

et al. 2021

Clinical and Translational Dis

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Biomarkers such as clinical characteristics and laboratory indices have been widely used in many respiratory diseases, such as chronic airway diseases, lung infection, lung cancer and acute respiratory distress syndrome. As such, various non‐invasive and invasive samples, such as blood, urine, induced sputum, bronchoalveolar lavage fluid and lung biopsy are currently being collected. Omics‐based discovery strategies facilitate the identification of next‐generation candidate biomarkers. The identification and integration of multiple omics biomarkers (genomics, epigenomics, proteomics, metabolomics and radiomics) can answer many unsolved questions about respiratory diseases. The ultimate realization of the translation of novel biomarkers into clinical practice is a major challenge because good biomarkers must be sensitive, technically feasible, non‐invasive, non‐expensive and most importantly, validated. The four stages of clinical trial design in the development of biomarkers are also discussed. The concept of systems biology not only focuses on novel molecular biomarkers but also integrates clinical, laboratory and omics information as a whole. Finally, developing multiple biomarker panels is a goal to improve the healthcare of respiratory diseases.

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Is NMR-based metabolomic analysis of exhaled breath condensate accurate?

Cited by 22 publications

References 18 publications

Nuclear magnetic resonance-based metabolomics of exhaled breath condensate: methodological aspects

Nuclear magnetic resonance-based metabolomics of exhaled breath condensate: methodological aspects

Exhaled breath condensate: a comprehensive update

Biomarkers for respiratory diseases: Present applications and future discoveries

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