Is non-buffered DMEM solution a suitable medium for <i>in vitro</i> bioactivity tests?

Rohanová, Dana; Boccaccını, Aldo R.; Horkavcová, Diana; Bozděchová, Pavlína; Bezdička, Petr; Častorálová, Markéta

doi:10.1039/c4tb00187g

Cited by 63 publications

(42 citation statements)

References 20 publications

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“…EBSS contains inorganic salts (NaCl, KCl, CaCl 2 , MgSO 4 , NaHCO 3 , NaH 2 PO 4 ) and was successfully used for CaPO 4 crystallization (Termine and Eanes 1974). Other popular physiological solutions comprise Hanks' balanced salt solution (HBSS), which contains almost similar inorganic salts and glucose (Hanks and Wallace 1949;Shibata et al 2004;Marques et al 2003a;Mareci et al 2010), Eagle's minimum essential medium (MEM) and its variation Dulbecco's modified Eagle's medium (DMEM), which contain numerous bioorganic (alanine, aspartic acid, glycine, biotin, vitamin C, folic acid, riboflavin) and inorganic (CaCl 2 , KCl, NaCl, NaH 2 PO 4 ) components (Meuleman et al 2006;Touny et al 2011;Coelho et al 2000;Mandel and Tas 2010;Rohanová et al 2014), phosphate-buffered saline (PBS) that contains only inorganic (CaCl 2 , MgCl 2 , KCl, KH 2 PO 4 , NaCl, NaH 2 PO 4 ) components (Gao et al 2006;Lichtenauer et al 2011). Furthermore, artificial saliva Ionta et al 2014;Okulus et al 2014), synthetic urine (Assimos 2013Dbira et al 2015) and simulated milk ultrafiltrate (SMUF) (Jenness and Koops 1962;Spanos et al 2007;Gao et al 2010a, b) solutions are available.…”

Section: Biomimetic Crystallization Of Capomentioning

confidence: 99%

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Dorozhkin¹

2017

Morphologie

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Section: Biomimetic Crystallization Of Capomentioning

confidence: 99%

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Dorozhkin¹

2017

Morphologie

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“…Therefore, in the literature, attempts to substitute SBF with the commercially available cell culture media, i.e. HEPES-free Dulbecco's modified Eagle medium (DMEM) solution, have been reported [15,17]. Apart from the inorganic part of blood serum, this solution contains also the organic component (vitamins, amino acids, etc.).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, DMEM can be supplemented with foetal bovine serum (FBS), containing additional organic species, such as proteins, to obtain complete growth medium for certain cell lines. The medium can be carbonate buffered as the test is performed in a 37°C incubator under a humidified atmosphere of 95% air and 5% CO 2 , which better simulates the in vivo environment [15,17].…”

Section: Introductionmentioning

confidence: 99%

A new insight into in vitro behaviour of poly(ε-caprolactone)/bioactive glass composites in biologically related fluids

et al. 2017

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In the present work, the role of content, size and chemical composition of gel-derived bioactive glass particles from the SiO 2 -CaO-P 2 O 5 system in modulating the in vitro bioactivity, osteoinductive properties and long-term (up to 15 months) degradation behaviour of poly(e-caprolactone)-based composite films was investigated. Bioactivity was assessed in simulated body fluid (SBF) and HEPES-free Dulbecco's modified Eagle medium (DMEM) supplemented with 10% foetal bovine serum (FBS), while hydrolytic degradation tests were performed in phosphate buffer saline. Obtained composite films showed excellent calcium phosphate (CaP) layer forming ability in both SBF and DMEM-10% FBS. However, kinetics of bioactivity process strongly depended on the type of medium used. The layer of amino acids and proteins, derived from cell culture medium, on the surfaces of composites created barrier that inhibited release of the ions on the one hand, while increasing nucleation density of calcium phosphates, affecting the morphology of formed CaP layers on the other. The presence of bioactive glass fillers was shown to impart osteoinductive properties to obtained films, supporting osteoblast attachment and proliferation, as well as stimulating cell differentiation and also matrix mineralization process in vitro. We showed that kinetics of bioactivity process and also osteoinductive properties of composite films could be easily modulated with the use of different contents and chemical compositions of fillers. The results showed that modification of PCL matrix with bioactive glass particles accelerated its degradation. We proved that the degradation rate of composites could be controlled and optimized for bone regeneration, in particular by using bioactive fillers causing different calcium phosphate layer forming ability on the surfaces of composites, depending on particle size and chemical composition. We have presented new opportunities to design and obtain multifunctional composites with tunable degradation and bioactivity kinetics, as well as biological properties that can meet complex requirements of bone tissue engineering.

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“…7a). This phenomenon could be due to the interaction between BGN and DMEM causing an amorphous Ca-P layer formation on the surface of the particles, 51 One approach to ensure continuous ion-release is to incorporate metallic elements into the silicate network. The SiO 4 tetrahedron is the basic building unit of the silicate glasses and the tetrahedron can bond to each other through Si-O-Si bonds.…”

mentioning

confidence: 99%

“…50 However, DMEM intrinsically contains abundant levels of potassium, calcium, and amino acids, leading to possible interaction with the tested material over time. 51 In this study, the assessment medium (DMEMWater mixture) contained Ca 2+ ions at a concentration of $6.5 mg mL À1 . It is notable that Ca 2+ ions were released from BGN-30 during the rst 1 hour immersion while the calcium concentration in the medium decreased in the following 3 days of immersion (Fig.…”

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confidence: 99%

Timing of calcium nitrate addition affects morphology, dispersity and composition of bioactive glass nanoparticles

et al. 2016

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Bioactive glass nanoparticles (BGN) are promising materials for a number of biomedical applications. Many parameters related to the synthesis of BGN using sol-gel methods can affect their characteristics. In this study, the influence of timing of calcium nitrate (calcium precursor) addition during processing on BGN characteristics was investigated. The results showed that the addition timing could affect the morphology, dispersity and composition of BGN. With delayed addition of calcium nitrate, larger, more regular and better dispersed BGN could be synthesized while the gap between nominal and actual compositions of BGN was widened. However, the addition timing had no significant influence on structural characteristics, as BGN with different addition-timing of calcium nitrate exhibited similar infrared spectra and amorphous nature. The results also suggested that monodispersed BGN could be synthesized by carefully controlling the addition of calcium nitrate. The synthesized monodispersed BGN could release Si and Ca ions continuously for up to at least 14 days. They also showed in vitro bioactivity and non-cytotoxicity towards rat bone marrow-derived mesenchymal stem cells (rBMSCs). In conclusion, the timing of calcium precursor addition is an essential parameter to be considered when producing BGN which should exhibit monodisperse characteristics for biomedical applications.

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Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests?

Cited by 63 publications

References 20 publications

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

A new insight into in vitro behaviour of poly(ε-caprolactone)/bioactive glass composites in biologically related fluids

Timing of calcium nitrate addition affects morphology, dispersity and composition of bioactive glass nanoparticles

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