Is OSNA better than imprint cytology for intraoperative diagnosis of cancer involvement of axillary sentinel node in breast cancer?

Tomás, Miguel Ángel Luna; Solà-Suárez, M.; Mariscal-Martínez, A.; Castella-Fernández, E.; Martínez‐Román, Sergio; Miguel, Iciar Pascual; Quiroga-García, V.; Julián-Ibáñez, J.F.

doi:10.1016/j.breast.2018.02.002

Cited by 1 publication

(1 citation statement)

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“…There are studies that report a clear advantage of the more popular imprints cytology (IC) with a calculated accuracy of 96.12% (14), whereas in other studies the OSNA method is found to be equally reliable (detection rates 11.8 vs 12.1%) when compared to other techniques such as cytokeratin immunohistochemistry (CK-IHC) (15). When cost is concerned there is limited data in the literature and mostly achieved from studies for breast cancer.…”

Section: Discussionmentioning

confidence: 99%

One step nucleic acid ampliﬁcation (OSNA) for detection of lymph node metastasis during robotic radical prostatectomy for prostate cancer: A pilot study

Argun

Mourmouris

Sağlıcan

et al. 2022

Arch Ital Urol Androl

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Introduction: The OSNA technique is based on reverse transcription loop-mediated DNA ampliﬁcation for the detection of cytokeratin 19 (CK19) messen-ger RNA (mRNA). The purpose of our paper, which represents the ﬁrst study in the literature, is to test the accuracy of this method in the detection of lymph node metastases in patients undergoing robotic radical prostatectomy with lymph node dis-section. Methods: Our cohort consisted of patients that have undergone robotic radical prostatectomy with extended lymph node dissec-tion. Lymph nodes were evaluated with imprint technique and then with frozen section examination. The remaining tissue was evaluated by OSNA method. Lymph nodes were deﬁned as ‘neg-ative’ or ‘positive’ according to mRNA copy number. Results: 7 patients and 25 lymph nodes were included in our cohort. Two patients were found negative with all pathology methods. In one patient the standard stains revealed a suspi-cious outcome but it was positive for micrometastasis with OSNA. In another patient the outcome was positive for standard stains and negative for OSNA. Finally, 2 patients were found positive for OSNA and negative for imprint methods. Conclusions: One Step Nucleic Acid Ampliﬁcation (OSNA) method using CK19 seems to fail in detection of lymph node metastases in prostate cancer patients undergoing radical prostatectomy and lymph node dissection.

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Section: Discussionmentioning

confidence: 99%