The main aim of this study was to evaluate the effects of the maize-cover crop rotation (Zm-cc) in the soil net nitrogen mineralisation (NNM) by measuring in situ NNM incubation in the upper soil (0-25 cm) during spring-summer (October-March) and autumn-winter (April-September) seasons and compared with other rotation in Mediterranean Central Chile. The study was carried out at 5 experimental fields (only irrigated in spring-summer period), where four common soil use or maizebased rotations were evaluated: permanent fallow (F-F); maize-fallow (Zm-F); maize-cover crop (Zm-cc); and permanent cover crops (cc-cc). In these fields were carried out NNM in situ determinations in F-F (n = 18), Zm-F (n = 31), Zm-cc (n = 43), and cccc (n = 51) combinations (totalise n = 143), which were collected during spring-summer and autumn-winter in different periods between 2011 and 2018. During the spring-summer period, it was found that the NNM was highest in the Zm-cc rotations with a mean value of 36 kg N ha −1 , whereas the lowest NNM values were in F-F soil use with and mean of 6 kg N ha −1. In contrast, during autumn-winter season, the NNM was highest in the F-F soil use with a mean of 34 kg N ha −1 , while the lowest NNM values were found in Zm-cc rotation with a mean of − 38 kg N ha −1. During the spring-summer period, the Zm-cc rotation had the highest NNM values because cc increased the soil organic matter (SOM) content for microbial activity, whereas in autumnwinter, the Zm-cc rotation had the lowest NNM values because the cc added fresh SOM that increased N immobilisation process.