Is Platelet-Rich Plasma a Future Therapy in Pain Management?

Knezevic, Nebojsa Nick; Candido, Kenneth D.; Desai, Ravi; Kaye, Alan D.

doi:10.1016/j.mcna.2015.08.014

Cited by 60 publications

(55 citation statements)

References 109 publications

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“…They have the ability to store numerous growth factors (GFs), enzymes and other bioactive molecules, which are rapidly released following platelet activation (2)(3)(4). Platelets are also able to stimulate certain key events in the reparative processes, such as replication of cells of mesenchymal origin (including fibroblasts, osteoblasts and endothelial cells), or chemotactic effects (2)(3)(4)(5).…”

Section: Introductionmentioning

confidence: 99%

“…Various methods have been developed for the topical use of platelet concentrates (PCs) in order to stimulate tissue growth and regeneration. Their clinical use in wound regeneration has gradually expanded over recent years, being applied in various fields such as ophthalmology, pain management, oral and maxillofacial surgery, orthopedics, plastic, periodontal and cardiac surgery and sports medicine (5)(6)(7)(8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

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Leukocyte depletion does not affect the inï¿½vitro healing ability of platelet rich plasma

et al. 2018

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The clinical use of platelet-rich plasma (PRP) containing or deprived of leukocytes remains a subject of debate and a controversial issue. It is not yet clear whether leukocyte content has a positive or negative effect on tissue healing processes. Several studies, conducted mainly in the orthopedic field, support the use of leukocyte-poor (LP) PRP, whereas other studies have not identified any significant differences between the use of LP and leukocyte-rich PRP. In the present study, the role of leukocytes contained in PRP was assessed to verify their effect on fibroblasts and endothelial cells, which have a leading role in the biological processes associated with wound healing (including angiogenesis and matrix remodeling). The original sample of PRP was divided into two aliquots, one of which remained unaltered, while the other was deprived of leukocytes. The two aliquots were used in tests in order to verify the effects of leukocytes on proliferation, wound healing and tube formation, and in molecular analyses of growth factor and enzyme content. The present results highlighted a substantial overlap between the two formulations. This may be explained by similar levels of growth factors (vascular endothelial growth factor, thrombospondin-1, interferon-γ, platelet-derived growth factor-BB, -AA and -B, tumor growth factor-β1, fibroblast growth factor 7 and tumor necrosis factor-α) and enzymes (gelatinases and plasminogen activators) in the two formulations. These results support the hypothesis that the ability of the PRP to affect the biological response of endothelial cells and fibroblasts does not rely on the presence of leukocytes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Leukocyte depletion does not affect the inï¿½vitro healing ability of platelet rich plasma

et al. 2018

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“…Platelet-Rich Plasma (PRP) is a low-cost procedure to deliver high concentrations of autologous GFs [12]. PRP has been defined as an autologous concentration of platelets that is 3 to 5 times greater than physiologic concentration of thrombocytes in whole blood [13]. PRP therapy represents a relatively new approach in regenerative medicine and accumulated considerable attention over the two last decades [14].…”

Section: Introductionmentioning

confidence: 99%

Effect of Platelet Rich Plasma (PRP) Injection on the Endocrine Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study

El‐Tahawy¹,

Rifaai²,

Saber³

et al. 2017

J Diabetes Metab

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Introduction: Diabetes mellitus is a global problem and several restoration approaches have been developed to induce beta (β) cells regeneration. Platelet rich plasma (PRP) is an autogenous and economical source of growth factors which nowadays used in the tissue repair. Aim:To test the hypothesis that PRP could play a role in the improvement of the structural changes occurred in the endocrine pancreas of experimentally-induced diabetic rats and the possible mechanisms through which PRP induced its effects to shed a light on the possible use of such application in the clinical field. Material and methods:Sixty male albino rats were used; 20 for obtaining the PRP and 40 were divided into 4 equal groups (10 rats each): control, PRP-group, diabetic group, PRP/diabetic group. Diabetes was induced by single intra-peritoneal injection of streptozotocin (50-60 mg/kg). The PRP was administered by SC injections in a dose of 0.5 mg/kg twice weekly for 3 weeks. Results:The diabetic group showed a significant increase in blood glucose levels compared to the control. Treatment with PRP significantly reduced the blood glucose levels compared to the diabetic group. The diabetic group showed variable marked morphological changes which diminished by the PRP administration. PRP/diabetic group had a significant increase in the mean number of pancreatic islets and β-cells/islet compared to the diabetic group. The islet cells appeared normal with scarcely seen vacuolations. The duct system showed several changes; stratifications, invagination of the surface epithelium to the underlying connective tissue, and sprouting of the ductal epithelial cells in between the lobules. Numerous small islets were noticed in a close association with the intralobular ducts. Small newly lobules with abundant connective tissue were organized. There were significant increases in the insulin immunopositive β-cells and PCNA positive cells in PRP/diabetic group compared to diabetic group. Conclusion:This study provides an evidence of the diabetic pancreatic islet regeneration in response to PRP treatment. The PRP stimulated islet cell regeneration and stimulated the induction of other sources of β-cells generation as the exocrine portion of the pancreas; ductal and acinar cells. In addition, PRP might put the pancreas into an environment similar to the postnatal developmental one where new lobules were formed. These will pave the future for a novel treatment for diabetes.

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“…Alphagranules of thrombocytes contain growth factors (GFs) associated with tissue healing, such as transforming growth factors (TGF) β1 and β2, insulin-like growth factor (IGF), and vascular endothelial growth factor (VEGF) (Lubkowska et al 2012). Therefore, they have been used for various purposes including orthopaedic, maxillofacial, periodontal, plastic surgeries, and sport medicine (Lubkowska et al 2012;Knezevic et al 2016;Richards et al 2016). They have been shown to have antimicrobial and healing properties leading to reduced postoperative swelling, lower consumption of analgesics and improved surgery outcomes (Cohn and Lockhart 2015;Knezevic et al 2016).…”

mentioning

confidence: 99%

“…Therefore, they have been used for various purposes including orthopaedic, maxillofacial, periodontal, plastic surgeries, and sport medicine (Lubkowska et al 2012;Knezevic et al 2016;Richards et al 2016). They have been shown to have antimicrobial and healing properties leading to reduced postoperative swelling, lower consumption of analgesics and improved surgery outcomes (Cohn and Lockhart 2015;Knezevic et al 2016). On the other hand, critics of PRP have pointed out a large number of variables used in the studies on the basis of which positive effects of PRP are assumed (Kuffler 2015).…”

mentioning

confidence: 99%

Concentrations of growth factors in platelet-rich plasma and platelet-rich fibrin in a rabbit model

Vokurka¹,

Göpfert²,

Blahutkova³

et al. 2016

Vet. Med. - Czech

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Platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) are platelet concentrates which have been used to improve healing and regeneration of damaged tissues. The concentration of growth factors greatly influences the final outcome of the treatment. For studying the effects of PRP and PRF, an animal model is required. The New Zealand White rabbit appears to be a good candidate for studying the features of these blood derivatives. This article presents for the first time the various preparation methods of blood derivatives with regard to concentration of growth factors -insulin-like growth factor, transforming growth factor and vascular endothelial growth factor. There was no statistically significant difference among the methods in the case of the first two factors. In the case of vascular endothelial growth factor, the lowest amount was detected in PRF. This study, even though performed on a limited number of animals, reports novel characterisation of the rabbit as an animal model for in vivo studies of the action of these blood derivatives.

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Is Platelet-Rich Plasma a Future Therapy in Pain Management?

Cited by 60 publications

References 109 publications

Leukocyte depletion does not affect the inï¿½vitro healing ability of platelet rich plasma

Leukocyte depletion does not affect the inï¿½vitro healing ability of platelet rich plasma

Effect of Platelet Rich Plasma (PRP) Injection on the Endocrine Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study

Concentrations of growth factors in platelet-rich plasma and platelet-rich fibrin in a rabbit model

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