Is the FVB/N mouse strain truly resistant to diet-induced obesity?

Abstract

C57Bl/6J mice are the gold standard animal model of diet‐induced obesity. These animals become obese with higher adiposity, blood fasting glucose, triglycerides, and total cholesterol when fed a high‐fat diet (HFD). Conversely, the FVB/N mouse line is thought to be resistant to diet‐induced obesity, with low or no weight gain and adiposity in response to a HFD. In this study, we investigated whether FVB/N mice are resistant or susceptible to metabolic disorder that is promoted by a HFD. Biometric parameters and blood chemistry were analyzed in C57Bl/6J and FVB/N mice that were fed a chow diet or HFD. Glucose and insulin sensitivity were assessed by performing the glucose tolerance test and measuring serum insulin/glucose and homeostasis model assessment‐insulin resistance. Metabolism‐related gene expression was investigated by real‐time reverse transcription polymerase chain reaction. Adipocyte morphology and liver steatosis were evaluated using standard histology. FVB/N mice had higher adiposity than C57Bl/6J mice that were fed a chow diet and were glucose intolerant. FVB/N mice that were fed a HFD presented higher insulin resistance and greater liver steatosis. Epididymal white adipose tissue exhibited severe inflammation in FVB/N mice that were fed a HFD. The FVB/N mouse strain is suitable for studies of diet‐induced obesity, and the apparent lack of a HFD‐induced response may reveal several strain‐specific events that are triggered by a HFD. Further studies of the FVB/N background may shed light on the complex multifactorial symptoms of obesity and metabolic syndrome.

Resistance to visceral obesity is associated with increased locomotion in mice expressing an endothelial cell‐specific fibroblast growth factor 1 transgene

“…Our data in the FVB control animals are supported by a study by Nascimento‐Sales et al. that report diet‐induced weight gain, steatosis, and insulin‐resistance. Importantly, all significant differences in the physiological, cellular and biochemical responses of the FGF1‐Tek strain were compared to the FVB Control animals.…”

“…), we and others (Nascimento‐Sales et al. ) have found that the FVB/N strain does indeed gain visceral adipose and exhibits characteristics of metabolic syndrome when administered a diet high in saturated fats. An FGF1 ELISA on visceral adipose tissue protein extracts from both FVB Control and FGF1‐Tek mice confirmed that FGF1 was elevated in visceral adipose in our model (Fig.…”

Modelos experimentais de obesidade: análise crítica do perfil metabólico e da aplicabilidade

“…Em nossa pesquisa, encontramos uma série de estudos (24) que utilizaram desta metodologia para indução da obesidade (Hvid, Blouin et al, 2013;Sanghez, Razzoli et al, 2013;Oliveira, Santos et al, 2014;Saleh, El-Maraghy et al, 2014;Donner, Elliott et al, 2015;Kulkarni, Jaji et al, 2015;Lai, Chen et al, 2015;Sladek, Stevens et al, 2015;Baldassano, Amato et al, 2016;Ferramosca, Conte et al, 2016;Grasa-Lopez, Miliar-Garcia et al, 2016;Marin, Rosso et al, 2016;Tung, Lin et al, 2016;Ezquer, Bahamonde et al, 2017;Nascimento-Sales, Fredo-Da-Costa et al, 2017;Ojo, Simenson et al, 2017;Oliveira, Chaves et al, 2017;Porras, Nistal et al, 2017;Sadowska, Gebczynski et al, 2017;Sharma, Kim et al, 2017;Xu, Ye et al, 2017;Li, Feng et al, 2018;Tasyurek, Eksi et al, 2018;Watanabe, Kumazaki et al, 2018) (Tabela 1).…”

“…da linhagem C57BL/6J, que possuem maior prédisposição ao ganho de peso pelo consumo excessivo de calorias(Nascimento-Sales, Fredo-Da-Costa et al, 2017). Considerado o modelo animal padrão ouro da obesidade induzida por dieta, esses animais se tornam obesos com maior adiposidade, glicemia de jejum, triglicérides e colesterol total quando alimentados com dieta hiperlipídica (Nascimento-Sales, Fredo-Da-Costa et al,2017).Embora seja um modelo simples de indução da obesidade, as dietas manipuladas com formulações constantes de gordura e carboidratos trazem a desvantagem de não reproduzir bem o hábito de escolha da alimentação da maioria da população humana(Barrett, Mercer et al, 2016;Marin, Rosso et al, 2016; Xu, Ye et al, 2017).…”

Resveratrol-Induced Effects on Body Fat Differ Depending on Feeding Conditions

“…In general, overfeeding rodents results in increased weight gain and body fat accumulation, although this response very much depends on the rodent strain [ 13 , 14 , 15 ]. Thus, high-fat diet feeding leads to increases in animal body weight and body fat percentage, as compared with those of the normal diet-fed counter partners, either in mice [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ] or in rats [ 25 , 26 , 27 ] ( Table 1 ).…”