Is the protection of photosynthesis related to the mechanism of quinclorac resistance in Echinochloa crus-galli var. zelayensis?

Gao, Yuan; Pan, Xukun; Sun, Xutao; Li, Jun; Dong, Liyao

doi:10.1016/j.gene.2018.10.022

Cited by 10 publications

(10 citation statements)

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“…[17][18][19] As quinclorac has a complex mode of action, quinclorac resistance may involve one or more of nonactivation of ethylene biosynthesis, enhanced cyanide (HCN) detoxification or high antioxidative capacity. 4,17,[20][21][22][23] For example, activities of antioxidative enzymes (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase) in shoots of quinclorac-tolerant rice was higher than those in quinclorac-susceptible E. oryzicola. 24 Lower levels of induced gene expression and/or activity of 1-aminocyclopropane-1-carboxylic acid synthase (ACS) and 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) genes can be associated with quinclorac resistance in grass species like E. crus-galli var.…”

Section: Introductionmentioning

confidence: 99%

“…Early studies using 14 C‐labelled quinclorac revealed that the resistance in some species, like E. crus‐galli and Galium spurium , was not due to differences in quinclorac uptake, translocation or metabolism 17–19 . As quinclorac has a complex mode of action, quinclorac resistance may involve one or more of nonactivation of ethylene biosynthesis, enhanced cyanide (HCN) detoxification or high antioxidative capacity 4,17,20–23 . For example, activities of antioxidative enzymes (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase) in shoots of quinclorac‐tolerant rice was higher than those in quinclorac‐susceptible E. oryzicola 24 .…”

Section: Introductionmentioning

confidence: 99%

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Exploring quinclorac resistance mechanisms in Echinochloa crus‐pavonis from China

Yang

Han

Cao

et al. 2020

Pest Management Science

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BACKGROUND: Barnyardgrass (Echinochloa spp.) is a global weed in rice fields. Quinclorac is commonly used to control barnyardgrass. However, due to persistent use, quinclorac resistance has evolved. We obtained quinclorac-susceptible (QS) and-resistant (QR1, QR2) lines from the progeny of a single resistant E. crus-pavonis for a resistance mechanism study. RESULTS: Line QR1 exhibited resistance to high quinclorac rates (up to 6400 g ha −1), whereas line QR2 exhibited a resistance/ susceptibility segregation ratio of 3:1 at the field or lower rates (400, 100 g ha −1). Intriguingly, a lower level of 14 C-quinclorac metabolism and hence a higher level of 14 C-quinclorac translocation was observed in QR1 than QS plants. The basal expression levels of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) and ACC oxidase 2 (ACO2) genes did not differ significantly between the QR1 and QS lines. However, more expression of ACS and ACO genes was induced by quinclorac treatment in QS than in QR1. Basal levels of ⊎-cyanoalanine synthase (⊎-CAS) gene expression were similar in QS and QR1 plants, but a greater level of down-regulation was detected in QS than in QR1 plants after quinclorac treatment. CONCLUSION: These results indicate QR plants are less responsive to quinclorac than QS plants in terms of up-regulating quinclorac metabolism and ethylene synthesis. Resistance in this E. crus-pavonis line is likely controlled by a single major gene, involving possibly an alteration in auxin signal perception/transduction to the ethylene biosynthesis pathway. The ⊎-CAS is unlikely to play a major role in quinclorac resistance in this particular population.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exploring quinclorac resistance mechanisms in Echinochloa crus‐pavonis from China

Yang

Han

Cao

et al. 2020

Pest Management Science

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“…Eight genes involved in auxin homeostasis were identified in the transcriptome sequencing database of E. crusgallli var. zelayensis (PRJNA430735) (Gao et al, 2019). EcYUCCA10 is responsible for auxin synthesis, five genes ( EcGH3.1 , EcGH3.3 , EcGH3.5 , EcGH3.11 and EcUGT ) are involved in IAA conjugation, and EcDAO is involved in auxin oxidation.…”

Section: Methodsmentioning

confidence: 99%

“…Although no differences in quinclorac metabolism have been found between resistant and susceptible Echinochloa spp., differential absorption, translocation, or metabolism have been linked to quinclorac resistance (Lovelace et al, 2007; Yasuor et al, 2012). Differences in the expression of photosynthesis‐related genes may contribute to the mechanism of resistance (Gao et al, 2019). More importantly, reduced ethylene production and subsequent cyanide by‐product appear to be the main mechanism of quinclorac resistance (Abdallah et al, 2006; Grossmann & Kwiatkowski, 1995).…”

Section: Introductionmentioning

confidence: 99%

Different regulation of auxin homeostasis would be a possible mechanism conferring quinclorac resistance in Echinochloa crusgalli var. zelayensis

Zhao

Pan

et al. 2022

Weed Research

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Differences in ethylene biosynthesis and cyanide detoxification have been reported to be mechanisms of quinclorac resistance in Echinochloa crusgalli var. zelayensis. Resistant phenotypes could be a consequence of the altered endogenous indole acetic acid (IAA) homeostasis induced by the herbicide. In this study, we determined the IAA content and expression levels of auxin homeostasis-related genes in susceptible and resistant biotypes of E. crusgalli var. zelayensis after quinclorac treatment. The results showed that the IAA content of JNNX-S (susceptible biotype) was significantly higher than that of SSXB-R (resistant biotype) after treatment with 50 μM quinclorac. To better understand this rise in IAA, the expression profiles of seven genes (one for auxin synthesis, five for IAA conjugation and one for IAA oxidation) and the biochemical activities of two oxidases involved in IAA homeostasis were measured. The expression of EcYUCCA10 was significantly higher in JNNX-S than in SSXB-R. The expression levels of the EcGH3s were significantly lower in JNNX-S than in SSXB-R. These expression profiles were consistent with the elevation of IAA levels in the susceptible biotype. In contrast, EcUGT and EcDAO were induced in each biotype, but a smaller increase was observed in SSXB-R than in JNNX-S. The enzymatic activities of IAA oxidases and peroxidases were higher in SSXB-R than in JNNX-S 24 h after treatment. It was inferred that altered expression of specific genes involved in IAA synthesis, conjugation and oxidation resulted in less IAA being induced in the resistant biotype, resulting in a lower ethylene burst and the associated quinclorac resistance. These results suggest novel layers of complexity in the mechanism of quinclorac resistance.

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“…However, only a limited number of herbicides are registered and can be used in rice fields. Among them, the auxin herbicide quinclorac was used for nearly 30 yr in China, resulting in high resistance frequency (Gao et al 2019). In addition, the ALS inhibitor bispyribac-sodium and ACCase inhibitor metamifop provided poor crop safety for some rice varieties, making them unpopular among rice growers in China.…”

Section: Introductionmentioning

confidence: 99%

Investigating the resistance levels and mechanisms to penoxsulam and cyhalofop-butyl in barnyardgrass (Echinochloa crus-galli) from Ningxia Province, China

Yang

Zhang

et al. 2021

Weed Sci

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Barnyardgrass (Echinochloa crus-galli) is a noxious grass weed which infests rice fields and causes huge crop yield losses. In this study, we collected twelve E. crus-galli populations from rice ﬁelds of Ningxia province in China and investigated the resistance levels to acetolactate synthase (ALS) inhibitor penoxsulam and acetyl-CoA carboxylase (ACCase) inhibitor cyhalofop-butyl. The results showed that eight populations exhibited resistance to penoxsulam and four populations evolved resistance to cyhalofop-butyl. Moreover, all of the four cyhalofop-butyl-resistant populations (NX3, NX4, NX6 and NX7) displayed multiple-herbicide-resistance (MHR) to both penoxsulam and cyhalofop-butyl. The alternative herbicides bispyribac-sodium, metamifop and fenoxaprop-P-ethyl cannot effectively control the MHR plants. To characterize the molecular mechanisms of resistance, we ampliﬁed and sequenced the target-site encoding genes in resistant and susceptible populations. Partial sequences of three ALS genes and six ACCase genes were examined. A Trp-574-Leu mutation was detected in EcALS1 and EcALS3 in two high-level (65.84- and 59.30-fold) penoxsulam-resistant populations NX2 and NX10, respectively. In addition, one copy (EcACC4) of ACCase genes encodes a truncated aberrant protein due to a frameshift mutation in E. crus-galli populations. None of amino acid substitutions that are known to confer herbicide resistance were detected in ALS and ACCase genes of MHR populations. Our study reveals the widespread of multiple-herbicide resistant E. crus-galli populations at Ningxia province of China that exhibit resistance to several ALS and ACCase inhibitors. Non-target-site based mechanisms are likely to be involved in E. crus-galli resistance to the herbicides, at least in four MHR populations.

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Is the protection of photosynthesis related to the mechanism of quinclorac resistance in Echinochloa crus-galli var. zelayensis?

Cited by 10 publications

References 65 publications

Exploring quinclorac resistance mechanisms in Echinochloa crus‐pavonis from China

Exploring quinclorac resistance mechanisms in Echinochloa crus‐pavonis from China

Different regulation of auxin homeostasis would be a possible mechanism conferring quinclorac resistance in Echinochloa crusgalli var. zelayensis

Investigating the resistance levels and mechanisms to penoxsulam and cyhalofop-butyl in barnyardgrass (Echinochloa crus-galli) from Ningxia Province, China

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