Is there a cold shock response in the Antarctic psychrophile Pseudoalteromonas haloplanktis?

Piette, Florence; Leprince, Pierre; Feller, Georges

doi:10.1007/s00792-012-0456-x

Cited by 25 publications

(10 citation statements)

References 12 publications

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“…Low temperatures promote increase in the reactive oxygen species due to increased oxygen solubility [ 10 , 41 ]; thus, counteraction against reactive oxygen species (ROS) formation is an important strategy to maintain the metabolic and genomic stability. It was reported that at low temperatures oxidative metabolism is repressed in order to decrease ROS production [ 10 , 42 ], however, in our studies we have not observed such an unequivocal response in carbon metabolism (while transcription of some of the TCA cycle genes was downregulated or remained constant, it did not hold true for other enzymes of this cycle or for the glycolysis pathway genes where some of them were upregulated). Thus, we can conclude that in response to cold stress, S. baltica activates oxidative stress protection in the rpoE2 -dependent pathway.…”

Section: Resultscontrasting

confidence: 91%

Adaptation of the Marine Bacterium Shewanella baltica to Low Temperature Stress

Kłoska

Cech

Sadowska

et al. 2020

IJMS

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Marine bacteria display significant versatility in adaptation to variations in the environment and stress conditions, including temperature shifts. Shewanella baltica plays a major role in denitrification and bioremediation in the marine environment, but is also identified to be responsible for spoilage of ice-stored seafood. We aimed to characterize transcriptional response of S. baltica to cold stress in order to achieve a better insight into mechanisms governing its adaptation. We exposed bacterial cells to 8 °C for 90 and 180 min, and assessed changes in the bacterial transcriptome with RNA sequencing validated with the RT-qPCR method. We found that S. baltica general response to cold stress is associated with massive downregulation of gene expression, which covered about 70% of differentially expressed genes. Enrichment analysis revealed upregulation of only few pathways, including aminoacyl-tRNA biosynthesis, sulfur metabolism and the flagellar assembly process. Downregulation was observed for fatty acid degradation, amino acid metabolism and a bacterial secretion system. We found that the entire type II secretion system was transcriptionally shut down at low temperatures. We also observed transcriptional reprogramming through the induction of RpoE and repression of RpoD sigma factors to mediate the cold stress response. Our study revealed how diverse and complex the cold stress response in S. baltica is.

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Section: Resultscontrasting

confidence: 91%

Adaptation of the Marine Bacterium Shewanella baltica to Low Temperature Stress

Kłoska

Cech

Sadowska

et al. 2020

IJMS

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“…However, the number of CSP genes varied widely in the genomes of 22 bacterial and archaeal psychrophiles, and no quantitative superiority was observed in CSP genes harbored by psychrophiles ( Siddiqui et al, 2013 ). Furthermore, proteomic profiles of psychrophile Pseudoalteromonas haloplanktis TAC 125 was reported to have no cold-induced proteins in response to a temperature shift from 18 to 4°C ( Piette et al, 2012 ). The additional copies of CSP coding gene CspA in Agromyces italicus DSM 16388, Gulosibacter molinativorax DSM 13485, and Humibacter albus DSM 18994, in contrast to the psychrophilic Cryobacterium species, did not enable them to become psychrophiles ( Manaia et al, 2004 ; Jurado et al, 2005 ; Vaz-Moreira et al, 2008 ).…”

Section: Discussionmentioning

confidence: 99%

Genomic Insights of Cryobacterium Isolated From Ice Core Reveal Genome Dynamics for Adaptation in Glacier

et al. 2020

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Glacier is the dominant cold habitat in terrestrial environments, providing a model ecosystem to explore extremophilic strategies and study early lives on Earth. The dominant form of life in glaciers is bacteria. However, little is known about past evolutionary processes that bacteria underwent during adaptation to the cryosphere and the connection of their genomic traits to environmental stressors. Aiming to test the hypothesis that bacterial genomic content and dynamics are driven by glacial environmental stressors, we compared genomes of 21 psychrophilic Cryobacterium strains, including 14 that we isolated from three Tibetan ice cores, to their mesophilic counterparts from the same family Microbacteriaceae of Actinobacteria. The results show that psychrophilic Cryobacterium underwent more dynamic changes in genome content, and their genomes have a significantly higher number of genes involved in stress response, motility, and chemotaxis than their mesophilic counterparts ( P < 0.05). The phylogenetic birth-and-death model imposed on the phylogenomic tree indicates a vast surge in recent common ancestor of psychrophilic Cryobacterium (gained the greatest number of genes by 1,168) after the division of the mesophilic strain Cryobacterium mesophilum . The expansion in genome content brought in key genes primarily of the categories “cofactors, vitamins, prosthetic groups, pigments,” “monosaccharides metabolism,” and “membrane transport.” The amino acid substitution rates of psychrophilic Cryobacterium strains are two orders of magnitude lower than those in mesophilic strains. However, no significantly higher number of cold shock genes was found in psychrophilic Cryobacterium strains, indicating that multi-copy is not a key factor for cold adaptation in the family Microbacteriaceae, although cold shock genes are indispensable for psychrophiles. Extensive gene acquisition and low amino acid substitution rate might be the strategies of psychrophilic Cryobacterium to resist low temperature, oligotrophy, and high UV radiation on glaciers. The exploration of genome evolution and survival strategies of psychrophilic Cryobacterium deepens our understanding of bacterial cold adaptation.

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“…In response to sudden exposure to lower temperatures, both mesophiles and psychrophiles up‐ or down‐regulate the expression of a significant number of genes, a process termed the cold‐shock response. Recently, the concept of cold‐shock response in psychrophiles sensu stricto has been called into question, with proteomic profiles of the true psychrophile Pseudoalteromonas haloplanktis TAC125 showing that no cold‐induced proteins were synthesized in response to a temperature shift from 18 to 4°C and that cold‐repressed proteins matched those observed when this strain was maintained at 4°C . However, it is noted that true psychrophiles such as P. haloplanktis may survive at temperatures as low as −20°C, and the technical challenges with replicating rapid temperature downshifts under laboratory conditions, make it difficult to test the plausibility of a cold‐shock response with a temperature downshift over this extreme temperature range .…”

Section: Molecular Adaptationmentioning

confidence: 99%

Some like it cold: understanding the survival strategies of psychrophiles

et al. 2014

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Much of the Earth's surface, both marine and terrestrial, is either periodically or permanently cold. Although habitats that are largely or continuously frozen are generally considered to be inhospitable to life, psychrophilic organisms have managed to survive in these environments. This is attributed to their innate adaptive capacity to cope with cold and its associated stresses. Here, we review the various environmental, physiological and molecular adaptations that psychrophilic microorganisms use to thrive under adverse conditions. We also discuss the impact of modern "omic" technologies in developing an improved understanding of these adaptations, highlighting recent work in this growing field.

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Is there a cold shock response in the Antarctic psychrophile Pseudoalteromonas haloplanktis?

Cited by 25 publications

References 12 publications

Adaptation of the Marine Bacterium Shewanella baltica to Low Temperature Stress

Adaptation of the Marine Bacterium Shewanella baltica to Low Temperature Stress

Genomic Insights of Cryobacterium Isolated From Ice Core Reveal Genome Dynamics for Adaptation in Glacier

Some like it cold: understanding the survival strategies of psychrophiles

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