Is there a correlation between paternal age and aneuploidy rate? An analysis of 3,118 embryos derived from young egg donors

Abstract

Objective: To investigate a possible correlation between chromosomal aberrations and paternal age, analyzing embryos derived from young oocyte donors, with available preimplantation genetic testing for aneuploidy results from day 5/6 trophectoderm biopsy obtained by next-generation sequencing for all 24 chromosomes. Design: Retrospective cohort study. Setting: Canadian fertility centre. Patient(s): A total of 3,118 embryos from 407 male patients, allocated into three paternal age groups: group A, %39 years (n ¼ 203); group B, 40-49 years (n ¼ 161); group C, R50 years (n ¼ 43). Intervention(s): None. Main Outcome Measure(s): The primary outcomes were aneuploidy, euploidy, mosaicism, and blastocyst formation rates. Secondary endpoints were comparison of specific chromosome aneuploidy, segmental and complex (involving two chromosomes þ mosaicism >50%) aneuploidy, and analysis of overall percentage of chromosomal gains and losses within each group. Result(s):The study included 437 in vitro fertilization (IVF) antagonist cycles using 302 oocyte donors in which preimplantation genetic testing for aneuploidy was performed. Overall, 70.04% of embryos were euploid, 13.9% were aneuploid, and 16.06% were mosaic. No significant differences among paternal age groups A, B, and C were found in euploidy rates (69.2%, 70.6%, 71.4%, respectively), aneuploidy rates (14.7%, 12.8%, 13.9%, respectively) or mosaicism rates (16.1%, 16.6%, 13.6%; respectively). The fertilization rate was lower in group C compared with group B (76.35% vs. 80.09%). No difference was found in blastocyst formation rate between the study groups (median 52% [interquartile range, 41%, 67%] vs. 53% [42%, 65%] vs. 52% [42%, 64%], respectively). A generalized linear mixed model regression analysis for embryo ploidy rates found older oocyte donor age to be independently associated with embryo aneuploidy (odds ratio ¼ 1.041; 95% CI, 1.009-1.074). The rate of segmental aneuploidies was significantly higher in the older versus younger paternal age group (36.6% vs. 19.4%). Conclusion(s):No association was found between paternal age and aneuploidy rates in embryos derived from IVF cycles using young oocyte donors, after adjusting for donor, sperm, and IVF cycle characteristics. Advanced paternal age R 50, compared with younger paternal ages, was associated with a lower fertilization rate and increased rate of segmental aberrations. (Fertil Steril Ò 2020;114: 293-300. Ó2020 by American Society for Reproductive Medicine.) El resumen está disponible en Español al final del artículo.

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“…Female age is a prominent factor affecting the outcome of ICSI-IVF [19,20]. However, male age had no signi cant effect on the rate of aneuploidy embryos [21]. The female age in this study ranged from 26 to 37, of which 16 were under 35 years old and 6 were over 35 years old.…”

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“…As for single-gene perturbations, several genes have been suggested to be implicated in POI (Table 1), some of them located on the X-chromosome and others on autosomes [4,8,9]. 1 See the main text for the full name of each gene. Full review of the genes can be found in references [4,8,9].…”

“…The importance of issues related to ovarian aging has been increasing progressively over the past several decades since, increasingly, more couples in all developed countries choose to postpone parenthood to more advanced female ages [1]. This trend is associated with an increasing rate of aneuploidy in oocytes, causing chromosomal abnormalities in embryos resulting from natural conception, conventional in vitro fertilization (IVF), or intracytoplasmic sperm injection (ICSI) [2,3].…”

“…This trend is associated with an increasing rate of aneuploidy in oocytes, causing chromosomal abnormalities in embryos resulting from natural conception, conventional in vitro fertilization (IVF), or intracytoplasmic sperm injection (ICSI) [2,3]. On the other hand, no association has been found between advanced male age and aneuploidy rates in embryos derived from IVF/ICSI attempts using oocytes from young donors [1].…”

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