Is there a relationship between time-lapse parameters and embryo sex?

Bronet, F.; Nogales, M.; Martínez, E.; Ariza, M.; Rubio, Carmen; García-Velasco, J.A; Meseguer, Marcos

doi:10.1016/j.fertnstert.2014.10.050

Cited by 45 publications

(27 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…When comparing the timing of cell divisions of embryos derived from sexed-sperm to that of conventionally derived embryos, no statistical difference was discernible, which was independent from the sex. In contrast, male human embryos have been found to develop at a significantly faster rate than female embryos 67,68 . Moreover, there was no increase in the percentage of apoptotic cells in embryos derived from sexed-sperm compared to the conventional sperm derived embryos, suggesting that sperm sexing does not cause downstream cell death beyond the blastocyst stage.…”

Section: Discussionmentioning

confidence: 89%

Bovine Sperm Sexing Alters Sperm Morphokinetics and Subsequent Early Embryonic Development

Steele

Makri

Maalouf

et al. 2020

Sci Rep

View full text Add to dashboard Cite

In artificial insemination the use of sex-sorted bovine sperm results in reduced conception, the causes of which are only partly understood. Therefore, we set out to investigate the effects of sexing on bovine sperm function and early embryonic development. Computer-assisted semen analysis (CASA) of sperm of the same bulls (n = 5), before and after sexing, demonstrated significantly reduced fast (A) and slow (B) progressively motile sperm (p < 0.05) after sexing. Sexed-sperm also revealed significantly less hyperactivated sperm (p < 0.05). As shown by time-lapse videomicroscopy of in vitro produced embryos (n = 360), embryos derived from sexed-sperm displayed significantly increased incidences of arrest at the 4-cell stage (p < 0.05). The relative risk for shrinkage/fusion of blastomeres with subsequent lysis was 1.71 times higher in the embryos derived from sexed-sperm as compared to conventional embryos (p < 0.05) resulting in significantly reduced blastocyst rates (p < 0.001). The relative risk for cleavage was 2.36 times lower in the embryos derived from sex-sorted sperm (p < 0.001). Additionally, sexedsperm-derived embryos showed reduced survival times (hazard ratio HR = 1.54, p < 0.001) which were bull dependent (p < 0.001). However, the percentage of apoptotic cells was similar to conventional embryos. Furthermore, embryos derived from sexed-sperm were found to reach developmental stages at similar timings as conventional embryos. our results suggest that reduced conception rates after sexing are due to altered sperm morphokinetics, decreasing the chance of sperm to reach and fertilise the oocyte, and aberrant early embryonic development.Flow cytometric sorting of spermatozoa is based on differences in the amount of DNA between X and Y bearing sperm, which is detected using the DNA-binding stain Hoechst 33342 1-3 . Due to low sperm numbers, sexed-sperm were primarily first used for in vitro fertilization (IVF) 4-6 . Further adaptions at Colorado State University enabled greater sorting efficiency, with the first commercial license for sexed-sperm use in artificial insemination (AI) issued in 2003 7 .The use of AI has grown to over 130 million artificial inseminations in the dairy industry worldwide annually, with 6% of those being sexed-sperm. In heifers, sexed-sperm accounted for 1.4%, of all registered US Holstein breedings in 2006, 9.5% in 2007 and 17.8% in 2008. In cows in the US, sexed-sperm were used in 0.1% of all breedings in 2006, 0.2% in 2007 and 0.4% in 2008 8 . In 2016, more than 4.5 million straws of sexed semen were processed in the US, over 90% being of dairy sire origin 9 . This increased demand in the dairy industry is because male calves are of low economic value and are associated with a higher risk of dystocia compared to heifer calves 8,10 . Additionally, the use of sexed-sperm in AI enables faster herd expansion, shortened generation intervals and an increased rate of genetic gain 11,12 .Despite the economic advantages of the use of sexed-semen, its routine use has been limited to da...

show abstract

Section: Discussionmentioning

confidence: 89%

Bovine Sperm Sexing Alters Sperm Morphokinetics and Subsequent Early Embryonic Development

Steele

Makri

Maalouf

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Such technology has captured the imagination of practitioners as both a research tool (Costa-Borges et al, 2016;Hardarson et al, 2015;Mölder et al, 2015), comparing embryos resulting from various clinical conditions (Balakier et al, 2016;Gurbuz et al, 2016;Hashimoto et al, 2016;Kaihola et al, 2016;Lindgren et al, 2016), and as a potential prognosticator in clinical practice (Adamson et al, 2016;Chen et al, 2016;Kong et al, 2016;Liu et al, 2016;Milewski et al, 2015;Mizobe et al, 2016;Rubio et al, 2014;VerMilyea et al, 2014;Wu et al, 2016aWu et al, , 2016bYang et al, 2014), although some studies negate this (Freour et al, 2015;Wu et al, 2016aWu et al, , 2016b. Bronet et al (2015) even found distinctive morphokinetic differences between male and female embryos. A few studies have purported to relate discriminating morphokinetics of euploid and aneuploid embryos (Campbell et al, 2013a(Campbell et al, , 2013bMinasi et al, 2016;Vera-Rodriguez et al, 2015), although this has been disputed (Rienzi et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Live births after embryo selection using morphokinetics versus conventional morphology: a retrospective analysis

Fishel¹,

Campbell²,

Montgomery³

et al. 2017

Reproductive BioMedicine Online

View full text Add to dashboard Cite

The increasing corpus of clinical studies using time-lapse imaging for embryo selection demonstrates considerable variation in study protocols and only limited-sized study cohorts. Outcome measures are based on implantation or clinical pregnancy; some predict blastulation from early cleavage-stage data, and few have evaluated live birth. Erroneously, most studies treat the embryos as independent variables and do not include patient or treatment variables in the statistical analyses. In this study, cohort size was 14,793 patients and 23,762 cycles. The incidence of live birth (n = 973 deliveries) after embryo selection by objective morphokinetic algorithms was compared with conventional embryology selection parameters (n = 6948 deliveries). A 19% increase in the incidence of live birth was observed when morphokinetic data were used to select embryos for the patient cohort aged younger than 38 years (OR 1.19 with 95% CI 1.06 to 1.34) using their own eggs, and an increase of 37% for oocyte recipients aged over 37 years (OR 1.370; 95% Cl 0.763 to 2.450). This is the largest study of the prospective use of time-lapse imaging algorithms in IVF reporting on live birth outcome, although the nature of purely a closed system versus standard incubation could not be assessed.

show abstract

“…Moreover, Alfarawati et al (2011) showed that male embryos developed significantly faster than females, and using time-lapse technology. Bronet et al (2015) found that two specific kinetic variables were able to predict a female embryo (timing between division to three cells and division to four cells [ > 2 h] and timing of morula formation [80.8-90.9 h]). In our current practice, we promote blastocyst transfer with the highest speed of development (=highest expansion rate on day 5).…”

Section: Discussionmentioning

confidence: 99%

Obstetric and perinatal outcomes of singletons after single blastocyst transfer: is there any difference according to blastocyst morphology?

Bouillon¹,

Celton²,

Kassem³

et al. 2017

Reproductive BioMedicine Online

View full text Add to dashboard Cite

A strong correlation between blastocyst morphology and implantation has been shown by many studies. The consequences and effects of assisted reproductive techniques on children's short and long-term health have always been a source of discussion. The obstetric and perinatal outcome of singletons according to blastocyst morphology has rarely been evaluated. The aim of this observational study is to determine whether a relationship exists between blastocyst morphology and obstetric and perinatal outcomes. A total of 799 singleton clinical pregnancies were analysed after transfer of a single fresh blastocyst on day 5 between 2006 and 2013. Blastocysts were divided into four groups based on their morphology on day 5: group 1 = good morphology blastocysts; group 2 = fair morphology blastocysts; group 3 = poor morphology blastocysts and group 4 = early (B1/B2) blastocysts. Obstetric and perinatal outcomes were compared between the four groups. After adjustment for some confounding variables, main obstetric and perinatal outcomes after transfer of blastocysts with poor morphological characteristics were not associated with increased adverse obstetric and perinatal events. Sex ratio was significantly higher in group 1 compared with groups 2, 3 and 4, and in Group 2 compared with Group 3 (P < 0.001) even after adjustment (P < 0.05).

show abstract

Is there a relationship between time-lapse parameters and embryo sex?

Cited by 45 publications

References 37 publications

Bovine Sperm Sexing Alters Sperm Morphokinetics and Subsequent Early Embryonic Development

Bovine Sperm Sexing Alters Sperm Morphokinetics and Subsequent Early Embryonic Development

Live births after embryo selection using morphokinetics versus conventional morphology: a retrospective analysis

Obstetric and perinatal outcomes of singletons after single blastocyst transfer: is there any difference according to blastocyst morphology?

Contact Info

Product

Resources

About