Is There a Threshold in the Antibacterial Action of Superhydrophobic Surfaces?

Ellinas, Kosmas; Kefallinou, Dionysia; Stamatakis, Kostas; Gogolides, Εvangelos; Tserepi, Angeliki

doi:10.1021/acsami.7b11402

Cited by 110 publications

(103 citation statements)

References 43 publications

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“…As reported in our previous work [34], the incorporation of a bactericidal agent is essential for ensuring long-term antibacterial action, in terms of constructing the ultimate, hybrid antibacterial surface. In this context, in order to determine the best antibacterial agent, copper and silver, two of the most frequently used bactericidal agents, were sputtered on untreated PMMA surfaces forming a 10 nm film.…”

Section: Bactericidal Activity Of Copper and Silvermentioning

confidence: 81%

“…The anti-adhesive properties of these superhydrophobic surfaces can perfectly contribute to their antibacterial behavior by delaying the bacterial biofilm formation. However, considering the bacterial potential to exponentially proliferate, even a limited bacterial number attached on superhydrophobic surfaces can progressively evolve to an extended bacterial population after several hours of immersion in bacterial solution (exceeding the as here studied 72 h), thereby shielding the superhydrophobic surfaces with only short-term antibacterial protection [34]. Therefore, in order to achieve long-term antibacterial activity, the engraftment of a bactericidal agent on superhydrophobic surfaces is attempted, which will secure the extermination of any remaining bacteria, fulfilling the bifunctional antibacterial role of the finally produced "hybrid" metal-sputtered superhydrophobic surfaces.…”

Section: Superhydrophobic Pmma Surfaces (C4f8 Fluorocarbon Layer)mentioning

confidence: 85%

“…Recent studies have focused on realizing such "smart" antibacterial surfaces. Among these, relaying on cases of proven anti-adhesive properties of superhydrophobic surfaces, including our previous work by Ellinas et al [34], in which bacterial repulsion was studied on superhydrophobic surfaces fabricated by plasma micro-nanotexturing, a variety of these bifunctional surfaces use superhydrophobic surfaces for ensuring the antibacterial role. A selection of the abovementioned bactericidal agents is integrated in the design of superhydrophobic surfaces for the completion of their antibacterial duality.…”

Section: Introductionmentioning

confidence: 99%

“…In this context, in our previous work [34] we presented a simple method for the fabrication of universal, metal-sputtered superhydrophobic "hybrid" antibacterial surfaces exhibiting both anti-adhesive and bactericidal long-term activity against Gram-negative bacteria of concentrations as high as 2 × 10 9 cfu/mL, one of the highest ever reported in the literature. One explanation for the beneficial anti-adhesive behavior of the proposed superhydrophobic surfaces is possibly their low friction properties [40].…”

Section: Introductionmentioning

confidence: 99%

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Optimization of Antibacterial Properties of “Hybrid” Metal-Sputtered Superhydrophobic Surfaces

et al. 2019

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Bacterial attachment and colonization to hygiene sensitive surfaces, both public and nosocomial, as well as in food industry areas, poses a serious problem to human healthcare. Several infection incidents are reported, while bacterial resistance to antibiotics is increasing. Recently, novel techniques for the design of antibacterial surfaces to limit bacterial spreading have emerged, including bifunctional antibacterial surfaces with antifouling and bactericidal action. In this context, we have recently developed smart, universal, metal-sputtered superhydrophobic surfaces, demonstrating both bacterial repulsion and killing efficacy. Herein, we present the optimization process that led to the realization of these “hybrid” antibacterial surfaces. To this end, two bactericidal agents, silver and copper, were tested for their efficiency against Gram-negative bacteria, with copper showing a stronger bactericidal action. In addition, between two low surface energy coatings, the fluorinated-alkyl self-assembled chlorosilane layer from perfluorinated octyltrichlorosilane (pFOTS) solution and the fluorocarbon layer from octafluorocyclobutane (C4F8) plasma were both approved for their anti-adhesive properties after immersion in bacterial solution. However, the latter was found to be more efficient when engrafted with the bactericidal agent in shielding its killing performance. Furthermore, the thickness of the plasma-deposited fluorocarbon layer was optimized, in order to simultaneously retain both the superhydrophobicity of the surface and its long-term bactericidal activity.

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Section: Bactericidal Activity Of Copper and Silvermentioning

confidence: 81%

Section: Superhydrophobic Pmma Surfaces (C4f8 Fluorocarbon Layer)mentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Optimization of Antibacterial Properties of “Hybrid” Metal-Sputtered Superhydrophobic Surfaces

et al. 2019

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“…Wetting control is important for many applications such as antifogging, antibacterial, self‐cleaning surfaces, as well as functional microfluidics . Additionally, superhydrophobic surfaces can find applications in frictionless motion of bodies inside fluids or reduced friction motion of drops inside microchannels …”

Section: Introductionmentioning

confidence: 99%

Motion of Drops with Different Viscosities on Micro‐Nanotextured Surfaces of Varying Topography and Wetting Properties

Sarkiris

Ellinas

Gkiolas

et al. 2019

Adv Funct Materials

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Superhydrophobic surfaces are extensively investigated in the literature, yet the phenomenon of drop motion on such surfaces and the corresponding friction properties of surfaces with different topography are not sufficiently analyzed. Here, drop motion on hydrophobic and superhydrophobic surfaces with different size topography is investigated for drops of largely varying viscosity (i.e., water and glycerol). The threshold force required to initiate drop movement is probed, the drop motion (velocity and acceleration) is analyzed, and the friction force on each surface is calculated. It is evident that as roughness increases, the threshold force to initiate 20 µL drop motion decreases; the lowest value for water is 17.9 ± 4.0 µN. For glycerol, the lowest threshold force value is 22.3 ± 5.9 µN. The results also indicate that this threshold force required for the initiation of the drop motion seems to be higher than that when the drop starts moving. Finally, this force (being proportional to the contact line) is expected to be about half smaller for 5 µL droplets. Water drops obtain higher velocities and accelerations by an order of magnitude compared to glycerol drops, which is attributed to the combinational effect of the higher hysteresis and the larger contact line of glycerol drops.

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Bacterial Colonization of Low‐Wettable Surfaces is Driven by Culture Conditions and Topography

Marguier

Poulin

Soraru

et al. 2020

Adv Materials Inter

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or food industry materials. [2-6] The initial events of biofilm formation include bacterial attachment to surface. This attachment depends on the environmental conditions (bacterial species, medium, temperature, etc) and on the material surface properties. Chemical composition, topography, mechanical properties as well as wettability (hydrophobicity and hydrophilicity), surface energy, and charge are the surface-related factors known to influence bacterial adhesion and biofilm development. [7,8] Some of them are specially examined for their great potential to create antibiofilm surfaces. [9] Surface energy is one of the factors involved in bacterial attachment. [10-13] More particularly, low-wettable especially superhydrophobic surfaces demonstrated some promising potential to reduce surface colonization by bacteria. [14-19] This prevention may be the result of reduced protein adsorption and entrapped air layer between the bacterial suspension and the surface [14,20-22] However, contradictory results are reported. [23,24] Aside from high differences in nature and wettability of the surfaces used in these studies, a serious reason is the common confusion regarding the distinction between bacterial retention and adhesion. Indeed, in Effect of surface low-wettability on bacterial colonization has become a prominent subject for the development of antibacterial coatings. However, bacteria's fate on such surfaces immersed in liquid as well as causal factors is poorly understood. This question is addressed by using a range of coatings with increasing hydrophobicity, to superhydrophobic, obtained by an atmospheric plasma polymer method allowing series production. Chemistry, wettability, and topography are thoroughly described, as well as bacterial colonization by in situ live imaging up to 24 h culture time in different liquid media. In the extreme case of superhydrophobic coating, substrates are significantly less colonized in biomolecule-poor liquids and for short-term culture only. Complex statistical analysis demonstrates that bacterial colonization on these low-wettable substrates is predominantly controlled by the culture conditions and only secondary by topographic coating's properties (variation in surface structuration with almost constant mean height). Wettability is less responsible for bacterial colonization reduction in these conditions, but allows the coatings to preserve colonization-prevention properties in nutritive media when topography is masked by fouling. Even after long-term culture in rich medium, many large places of the superhydrophobic coating are completely free of bacteria in relation to their capacity to preserve air trapping.

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Is There a Threshold in the Antibacterial Action of Superhydrophobic Surfaces?

Cited by 110 publications

References 43 publications

Optimization of Antibacterial Properties of “Hybrid” Metal-Sputtered Superhydrophobic Surfaces

Optimization of Antibacterial Properties of “Hybrid” Metal-Sputtered Superhydrophobic Surfaces

Motion of Drops with Different Viscosities on Micro‐Nanotextured Surfaces of Varying Topography and Wetting Properties

Bacterial Colonization of Low‐Wettable Surfaces is Driven by Culture Conditions and Topography

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