Is There an Answer? ‐ Coordinated by Frank Vella

Ascenzi, P.; Gradoni, Luigi; Visca, Paolo

doi:10.1080/15216540410001710101

Cited by 1 publication

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…iNOS-954C allele, but not the iNOS-954G allele, may have a higher affinity to a DNA binding protein and thus, translational activity and NOS mRNA levels were increased in the iNOS-954C allele resulting in overall enhanced NOS expression. This rationale is quite well in accordance with our findings of increased iNOS protein and mRNA level both in infection induced as well as mutation specific expression as previously observed by Kun et al [20] , Ascenzi P et al [72] and Chiwakata CB et al [73] . This also corroborates with our observation that iNOS-954C allele showed increased NO levels.…”

Section: Discussionsupporting

confidence: 93%

iNOS polymorphism modulates iNOS/NO expression via impaired antioxidant and ROS content in P. vivax and P. falciparum infection

et al. 2018

View full text Add to dashboard Cite

Nitric oxide (NO) has dicotomic influence on modulating host-parasite interplay, synchronizing physiological orchestrations and diagnostic potential; instigated us to investigate the plausible association and genetic regulation among NO level, components of oxidative stress, iNOS polymorphisms and risk of malaria. Here, we experimentally elucidate that iNOS promoter polymorphisms are associated with risk of malaria; employing mutation specific genotyping, functional interplay using western blot and RT-PCR, quantitative estimation of NO, total antioxidant content (TAC) and reactive oxygen species (ROS).Genotyping revealed significantly associated risk of P. vivax (adjusted OR = 1.92 and 1.72) and P. falciparum (adjusted OR = 1.68 and 1.75) infection with SNP at iNOS-954G/C and iNOS-1173C/T positions, respectively; though vivax showed higher risk of infection. Intriguingly, mutation and infection specific differential upregulation of iNOS expression/NO level was observed and found to be significantly associated with mutant genotypes. Moreover, P. vivax showed pronounced iNOS protein (2.4 fold) and mRNA (2.5 fold) expression relative to healthy subjects. Furthermore, TAC and ROS were significantly decreased in infection; and differentially decreased in mutant genotypes.Our findings endorse polymorphic regulation of iNOS expression, altered oxidant-antioxidant components and evidences of risk association as the hallmark of malaria pathogenesis. iNOS/NO may serve as potential diagnostic marker in assessing clinical malaria.

show abstract

Section: Discussionsupporting

confidence: 93%