Is there post-meiotic transcriptional activity during hemipteran spermiogenesis?

Alevi, Kaio Cesar Chaboli; Mendonaca, Priscila Pasqueetto; Pereira, Nathália Paiva; Rosa, João Aristeu da; Azeredo-Oliveira, Maria Tercília Vilela de

doi:10.1080/07924259.2014.889767

Cited by 9 publications

(7 citation statements)

References 45 publications

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“…Although nucleolar marks were observed in spermatids of M. brasiliensis, recently Alevi et al (2014b) suggested that the cells of hemipterans do not have transcriptional activity during spermiogenesis. The authors highlight the relationship of nucleolar markings with regions of constitutive heterochromatin and suggest that the nucleolus is inactivated by epigenetic factors and that the organelle chromatoid body supplies all transcriptional activity during cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

Study of nucleolar behavior during spermatogenesis in Martarega brasiliensis (Heteroptera, Notonectidae)

Pereira¹,

Alevi²,

Moreira³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Few cytogenetic studies have been undertaken using aquatic heteropterans and the nucleolar behavior of these insects has been described in only four species, Limnogonus aduncus, Brachymetra albinerva, Halobatopsis platensis, and Cylindrostethus palmaris. The nucleolus is a cellular structure related to biosynthetic 8989 Nucleolar behavior in Martarega brasiliensis ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 8988-8994 (2015) activity and it exhibits a peculiar behavior in the heteropterans of the Triatominae subfamily; it persists during all stages of meiosis. Thus, this study aims to analyze spermatogenesis in Martarega brasiliensis, with an emphasis on nucleolar behavior. Twenty M. brasiliensis adult males were used and collected from the Municipal reservoir in the city of São José do Rio Preto, São Paulo, Brazil. The species were fixed in methanol:acetic acid (3:1), then dissected, and the testicles were extracted, torn apart, and impregnated with silver ions. During prophase, the nuclei of M. brasiliensis were composed of the nucleolus and nucleolar corpuscles, which varied in number from one to four, emphasizing that this insect has great synthetic activity during meiosis. The analysis of cells in metaphase I showed that M. brasiliensis presents a nucleolar organizing region in at least one autosome. Furthermore, the phenomenon of nucleolar persistence was not observed. All spermatids presented nucleolar markings that varied in number and position according to the stage of elongation. Moreover, it was also possible to highlight the presence of a vesicle in spermatids. Thus, this paper describes the nucleolar behavior of M. brasiliensis and highlights important characteristics during spermatogenesis, thus, increasing the knowledge about the biology of these aquatic heteropterans.

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Section: Discussionmentioning

confidence: 99%

Study of nucleolar behavior during spermatogenesis in Martarega brasiliensis (Heteroptera, Notonectidae)

Pereira¹,

Alevi²,

Moreira³

et al. 2015

Genet. Mol. Res.

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“…Although the nucleolus is present during triatomine spermiogenesis, transcriptional activity does not occur, because it is inactivated by epigenetic factors (Severi-Aguiar and Azeredo-Oliveira, 2005;Alevi et al, 2014b;Borgueti et al, 2015). Therefore, all transcriptional activity necessary for cell differentiation during spermiogenesis is supported by the chromatoid body (CB) (Alevi et al, 2014b, Borgueti et al, 2015.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, all transcriptional activity necessary for cell differentiation during spermiogenesis is supported by the chromatoid body (CB) (Alevi et al, 2014b, Borgueti et al, 2015.…”

Section: Introductionmentioning

confidence: 99%

Presence of chromatoid bodies in the Rhodnius genus detected by cytochemical analysis

et al. 2016

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ABSTRACT. In addition to the epidemiological importance regarding the transmission of Chagas disease, triatomines are also important biological models for cellular studies, because they have holocentric chromosomes, post-reductional meiosis for sex chromosomes, and nucleolar persistence. Although the nucleolus is present during spermiogenesis, it was suggested that it is inactivated and all transcriptional activity necessary for cell differentiation is supported by the chromatoid body (CB). Thus, considering the importance of CB to triatomine spermatogenesis, this paper aims to analyze the presence of this organelle in the Rhodnius genus. The testicles of five adult R. neglectus and R. prolixus males were analyzed after semi- fine sections were performed, and the material was subjected to silver ion impregnation. The CB organelle was found in the spermatid of both species. Most CB studies focused on the Triatoma genus. This study described the existence of CB in Rhodnius, confirming thus the importance of this cytoplasmic organelle to spermiogenesis of these insect vectors of Chagas disease.

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“…20 Thus, it has been suggested that the nucleolus that persists during meiosis can be a structure responsible for forming the CB, because if it is active, all the transcribed RNA is directed to the formation of the CB. 17 Therefore, we describe the nucleolar persistence as a synapomorphy of the triatomines and suggest that further analysis should be conducted to assess whether the persistent nucleolus is really active, thus clarifying the importance of the persistence of the nucleolus for spermatogenesis of these vectors of Chagas disease.…”

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confidence: 99%

Nucleolar Persistence: Peculiar Characteristic of Spermatogenesis of the Vectors of Chagas Disease (Hemiptera, Triatominae)

Madeira

Borsatto

Lima

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. All species of triatomines are considered potential vectors of Chagas disease and the reproductive biology of these bugs has been studied by different approaches. In 1999, nucleolar persistence during meiosis was observed in the subfamily for the first time. Recently, it has been observed that all species within the genus Rhodnius exhibit the same phenomenon, suggesting that it may be a synapomorphy of the triatomines. Thus, this article aims to analyze the nucleolar behavior during spermatogenesis of 59 triatomine species. All analyzed species exhibited nucleolar persistence during meiosis. Recently, it has been suggested that nucleolar persistence may be fundamental for the spermatogenesis of these vectors, since it is related to the formation of the chromatoid body. Therefore, we emphasize that this phenomenon is a peculiarity of the Triatominae subfamily and that further studies are required to analyze whether the nucleolar material that persists is active.The Triatominae subfamily is composed of 150 species (148 living species and two fossils), grouped into 18 genera and five or six tribes. All triatomine species are considered potential vectors of Trypanosoma cruzi, the etiologic agent of Chagas disease. 1Chagas disease is a neglected disease that has no cure. The main way to minimize the incidence of this disease in human populations is through vector control.1 It is estimated that more than 5 million people are infected by T. cruzi, the parasite that causes Chagas disease, and that 70 million still live at risk, which places this illness among the most serious parasitic diseases in the world.1 Thus, improving the knowledge on several fields on triatomine vector potentiality (such as ecology 2 and biology 3 ) may provide important information for control measures.The reproductive biology of these bugs has been studied by different approaches, such as cytogenetic, 4 structural, 5 and ultrastructural 6 analysis. Furthermore, the spermatogenesis of the triatomines is characterized as cystic, 5,6 and it has been suggested that, during imaginal molt (transition from the fifth instar nymph to adult), the cell division is disrupted, aiming to reduce energy costs, and the differentiation into sperm is stimulated to ensure the paternity of the adult male. 7In 1999, Tartarotti and Azeredo-Oliveira, 8 while studying the spermatogenesis of Panstrongylus megistus and Panstrongylus herreri (= Panstrongylus lignarius), noted that these triatomines exhibited a different nucleolar behavior than the one described for other eukaryotes: the nucleolus persisted during all stages of meiosis. The authors characterized this phenomenon as nucleolar persistence.Recently, Alevi and others 9 have observed that all species within the genus Rhodnius feature nucleolar persistence during meiosis as well, and they suggested that analyses of the nucleolar behavior should be carried out in a large range of species of triatomines to examine whether that characteristic is a synapomorphy of the Triatominae subfamily.Thus, this ...

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Is there post-meiotic transcriptional activity during hemipteran spermiogenesis?

Cited by 9 publications

References 45 publications

Study of nucleolar behavior during spermatogenesis in Martarega brasiliensis (Heteroptera, Notonectidae)

Study of nucleolar behavior during spermatogenesis in Martarega brasiliensis (Heteroptera, Notonectidae)

Presence of chromatoid bodies in the Rhodnius genus detected by cytochemical analysis

Nucleolar Persistence: Peculiar Characteristic of Spermatogenesis of the Vectors of Chagas Disease (Hemiptera, Triatominae)

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