Is there still room to explore cyclodextrin glycosyltransferase-producers in Brazilian biodiversity?

Gomes, Andrea C.S.M.; Santos, Samuel Rodrigues Dos; Ribeiro, Maycon Carvalho; Cravo, Pedro Vitor Lemos; Vieira, José Daniel Gonçalves; Souza, Keili Maria Cardoso de; Amaral, André Corrêa

doi:10.1590/0001-3765201820170670

Cited by 2 publications

(1 citation statement)

References 22 publications

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“…SG2 was identified as B. patagoniensis. This species of bacteria was among those isolated and confirmed to be CGTase producer by Gomes et al [24]. The isolate from Lake Van (V3) was identified as B. agaradhaerens and was identified as being distinctly different than the other three isolates obtained from Lake Salda.…”

Section: Strain Isolation and Identificationsupporting

confidence: 61%

Isolation, Identification and Evaluation of Enzyme Production Capacity of CGTase Producing Bacteria from Lakes Salda and Van/Turkey

Paakkanen¹,

Budak²

2019

BioDiCon

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The aim of this study was isolation of CGTase positive microorganisms from lakes Salda and Van, both being extreme environments unique in character with high alkalinity pH>9.7. Four bacterial strains designated as SD5, SG2, SG3 and V3 were isolated by cultivation on selective Horikoshi agar and confirmed as CGTase producers. Microscopic inspection showed the four isolates to be Gram positive rods. Strains were identified as Bacillus based on DNA sequences of 16S rDNA region. Only the V3 and SG2 isolates could be resolved at species level identified as B. agaradhaerens and B. patagoniensis, respectively. Growth and CGTase production capacity of the strains were evaluated under shake flask conditions. All isolates achieved extracellular CGTase levels of 6-8 U/mL within 24 h of incubation. Growth curves of Bacillus sp. SG3, Bacillus sp. SD5, B. agaradhaerens V3 showed the bacteria to reach a plateau phase within 24-30 h however, extended fermentation up to 168 h showed B. patagoniensis SG2 to continue the growth cycle until the end of this period with higher CGTase activity observed. These four strains isolated which have thus been confirmed as CGTase positive bacteria can potentially be exploited as genetic sources for the synthesis of CGTase in industrial scale.

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Section: Strain Isolation and Identificationsupporting

confidence: 61%

Isolation, Identification and Evaluation of Enzyme Production Capacity of CGTase Producing Bacteria from Lakes Salda and Van/Turkey

Paakkanen¹,

Budak²

2019

BioDiCon

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Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities

Landriel

Castillo

Taboga

et al. 2019

An. Acad. Bras. Ciênc.

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Cyclodextrin glycosyltransferases (CgTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β-and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CgTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as Paenibacillus barengoltzii, and to unveil a complex origin for CGTase types during archaeal and bacterial evolution. We postulate that the α-CGTase activity represents the ancestral type, and that the γ-activity may have derived from β-CGTases.

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Is there still room to explore cyclodextrin glycosyltransferase-producers in Brazilian biodiversity?

Cited by 2 publications

References 22 publications

Isolation, Identification and Evaluation of Enzyme Production Capacity of CGTase Producing Bacteria from Lakes Salda and Van/Turkey

Isolation, Identification and Evaluation of Enzyme Production Capacity of CGTase Producing Bacteria from Lakes Salda and Van/Turkey

Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities

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